A5070886938- Quantum and electron transport phenomena
- Physics of Superconductivity and Magnetism
- Semiconductor Quantum Structures and Devices
- Cardiac electrophysiology and arrhythmias
- Ion channel regulation and function
- Magnetic properties of thin films
- RNA modifications and cancer
- Graphene research and applications
- Ubiquitin and proteasome pathways
- 14-3-3 protein interactions
- Analytical Chemistry and Sensors
- Electrochemical sensors and biosensors
- Semiconductor materials and devices
- Origins and Evolution of Life
- Ion Channels and Receptors
- Molecular Sensors and Ion Detection
- Advanced Condensed Matter Physics
- Receptor Mechanisms and Signaling
- CRISPR and Genetic Engineering
- Lanthanide and Transition Metal Complexes
- Cold Atom Physics and Bose-Einstein Condensates
- Topological Materials and Phenomena
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
City University of Hong Kong, Shenzhen Research Institute
2025
Shandong University
2022-2025
University of Science and Technology of China
2014-2024
Nankai University
2024
Henan University of Technology
2022-2024
Huazhong University of Science and Technology
2023-2024
First Affiliated Hospital of Gannan Medical University
2024
Tongji Hospital
2023-2024
Wuyi University
2024
Chinese PLA General Hospital
2024
We report the development of highly versatile fluorescent and photostable core−shell Ag@SiO2 nanocomposites, which allows researchers flexibility to incorporate just about any fluorophores outer silica shell by two simple methods. To show generality preparation technique, we have developed three different probes: an organic fluorophore (Rh800) a lanthanide probe doped (noncovalently linked) (Alexa 647) covalently linked shell. When compared control nanoparticles (nanobubbles), with silver...
Mucous cell hyperplasia and airway smooth muscle (ASM) hyperresponsiveness are hallmark features of inflammatory diseases, including asthma. Here, we show that the recently identified calcium-activated chloride channel (CaCC) TMEM16A is expressed in adult surface epithelium ASM. The epithelial expression increased asthmatics, particularly secretory cells. Based on this proposed functions CaCC, hypothesized inhibitors would negatively regulate both mucin secretion ASM contraction. We used a...
Abstract Limited understanding exists regarding how aging impacts the cellular and molecular aspects of human ovary. This study combines single-cell RNA sequencing spatial transcriptomics to systematically characterize ovarian aging. Spatiotemporal signatures eight types cells during are observed. An analysis age-associated changes in gene expression reveals that DNA damage response may be a key biological pathway oocyte Three granulosa subtypes five theca stromal subtypes, as well their...
A bright idea: 15-fold increase in fluorescence intensity occurs when the complex formed between Eu3+ and antibiotic tetracycline binds to hydrogen peroxide at neutral pH. The can be used determination of concentration H2O2, activity oxidases, glucose, also an optical sensor for H2O2.
Abstract Adipose tissue dysfunction is critical to the development of type II diabetes and other metabolic diseases. While monolayer cell culture has been useful for studying fat biology, 2D often does not reflect complexity tissue. Animal models are also problematic in that they expensive, time consuming, may completely recapitulate human biology because species variation. To address these problems, we have developed a scaffold-free method generate 3D adipose spheroids from primary or...
Mitochondrial dysfunction is an underlying pathology in numerous diseases. Delivery of diagnostic and therapeutic cargo directly into mitochondria a powerful approach to study treat these The triphenylphosphonium (TPP+) moiety the most widely used mitochondriotropic carrier. However, studies have shown that TPP+ not inert; conjugates uncouple mitochondrial oxidative phosphorylation. To date, all efforts toward addressing this problem focused on modifying lipophilicity TPP+-linker-cargo alter...
The human female reproductive lifespan significantly diminishes with age, leading to decreased fertility, reduced fertility quality and endocrine function disorders. While many aspects of aging in general have been extensively documented, the precise mechanisms governing programmed system remain elusive. Recent advancements omics technologies computational capabilities facilitated emergence multiomics deep phenotyping. Through application refinement various high-throughput methods, a...
Electron spin relaxation in bulk III-V semiconductors is investigated from a fully microscopic kinetic Bloch equation approach where all relevant scatterings, such as, the electron--nonmagnetic-impurity, electron-phonon, electron-electron, electron-hole, and electron-hole exchange (the Bir-Aronov-Pikus mechanism) scatterings are explicitly included. The Elliott-Yafet mechanism also incorporated. This offers way toward thorough understanding of electron both near far away equilibrium metallic...
To understand the main spin relaxation mechanism in graphene, we investigate with random Rashba field (RRF) induced by both adatoms and a substrate using kinetic Bloch equation approach. The charged adatoms, on one hand, enhance spin–orbit coupling locally and, other serve as Coulomb potential scatterers. Both effects contribute to limited D'yakonov–Perel' (DP) mechanism. In addition, RRF also causes spin-flip scattering, manifesting itself an Elliott–Yafet (EY)-like mechanisms are sensitive...
The K(ir) inward rectifying potassium channels have a broad tissue distribution and are implicated in variety of functional roles. At least seven classes (K(ir)1-K(ir)7) structurally related rectifier known, there no selective small molecule tools to study their function. In an effort develop K(ir)2.1 inhibitors, we performed high-throughput screen (HTS) more than 300,000 molecules within the MLPCN for modulators Here report one potent inhibitor, ML133, which inhibits with IC(50) 1.8 μM at...
Voltage-gated KCNQ1 (Kv7.1) potassium channels are expressed abundantly in heart but they also found multiple other tissues. Differential coassembly with single transmembrane KCNE beta subunits different cell types gives rise to a variety of biophysical properties, hence endowing distinct physiological roles for KCNQ1–KCNEx complexes. Mutations either or KCNE1 genes result diseases brain, heart, and the respiratory system. In addition complexities arising from existence five subunits, KCNE5,...
The DNA repair protein RAD52 is an emerging therapeutic target of high importance for BRCA-deficient tumors. Depletion synthetically lethal with defects in tumor suppressors BRCA1, BRCA2 and PALB2. also participates the recovery stalled replication forks. Anticipating that ssDNA binding activity underlies cellular functions, we carried out a throughput screening campaign to identify compounds disrupt RAD52-ssDNA interaction. Lead were confirmed as inhibitors biochemical assays. Computational...
Diverse functions of 14-3-3 proteins are directly coupled to their ability interact with targeted peptide substrates. RSX(pS/pT)XP and RXΦX(pS/pT)XP two canonical consensus binding motifs for representing the common modes, modes I II, between internal peptides. Using a genetic selection, we have screened random library identified group C-terminal motifs, termed SWTY, capable overriding an endoplasmic reticulum localization signal redirecting membrane cell surface. Here report that SWTY...
Tumor suppressor p53 is responsible for enforcing cell cycle checkpoints at G1/S and G2/M in response to DNA damage, thereby allowing both normal tumor cells repair before entering S M. However, with absent or mutated are able activate alternative signaling pathways that maintain the checkpoint, which becomes uniquely critical survival of such cells. We hypothesized abrogation G2 checkpoint might preferentially sensitize p53-defective DNA-damaging agents spare intact function. The tyrosine...
Objective— The main objective of this study is to define the mechanisms by which mitochondria control vascular smooth muscle cell (VSMC) migration and impact neointimal hyperplasia. Approach Results— multifunctional CaMKII (Ca 2+ /calmodulin-dependent kinase II) in mitochondrial matrix VSMC drove a feed-forward circuit with Ca uniporter (MCU) promote influx. MCU was necessary for activation (mtCaMKII), whereas mtCaMKII phosphorylated at regulatory site S92 that promotes entry. sufficient...
Transient receptor potential canonical 3/6/7 (TRPC3/6/7) are highly homologous receptor-operated nonselective cation channels. Despite their physiological significance, very few selective and potent agonists available for functional examination of these Using a cell-based high throughput screening approach, lead compound with the pyrazolopyrimidine skeleton was identified as TRPC6 agonist. Synthetic schemes its analogues were established, structural–activity relationship studies carried out....
Getting the picture: Citrate and other intermediates of citric acid cycle can be imaged by time-resolved fluorescence with a Eu3+ tetracycline complex as fluorescent probe (see picture). The time resolution enables discrimination between intermediates, enzymatic conversions are not needed.