A5074798370- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced biosensing and bioanalysis techniques
- Advanced Fluorescence Microscopy Techniques
- Photochemistry and Electron Transfer Studies
- Protein Interaction Studies and Fluorescence Analysis
- Analytical Chemistry and Sensors
- Plasmonic and Surface Plasmon Research
- Spectroscopy and Quantum Chemical Studies
- Advanced Biosensing Techniques and Applications
- Lanthanide and Transition Metal Complexes
- Electron Spin Resonance Studies
- Molecular Sensors and Ion Detection
- Biosensors and Analytical Detection
- Photonic and Optical Devices
- Electrochemical sensors and biosensors
- Mass Spectrometry Techniques and Applications
- DNA and Nucleic Acid Chemistry
- Nanocluster Synthesis and Applications
- Spectroscopy and Laser Applications
- Photoreceptor and optogenetics research
- Metal complexes synthesis and properties
- Quantum Dots Synthesis And Properties
- Luminescence and Fluorescent Materials
- Lipid Membrane Structure and Behavior
- Optical Imaging and Spectroscopy Techniques
University of Maryland, Baltimore
2013-2023
University of Baltimore
1999-2019
University of Mary
1992-2018
Fluor (United States)
2002-2018
University of Science and Technology of China
2014
University of Maryland, College Park
1990-2013
Fluorescence Innovations (United States)
1995-2012
University of Virginia Health System
2010
Argonne National Laboratory
2009-2010
Lumerical Solutions (Canada)
2009-2010
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTQuenching of fluorescence by oxygen. Probe for structural fluctuations in macromoleculesJoseph R. Lakowicz and Gregorio WeberCite this: Biochemistry 1973, 12, 21, 4161–4170Publication Date (Print):October 1, 1973Publication History Published online1 May 2002Published inissue 1 October 1973https://pubs.acs.org/doi/10.1021/bi00745a020https://doi.org/10.1021/bi00745a020research-articleACS PublicationsRequest reuse permissionsArticle...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTQuenching of protein fluorescence by oxygen. Detection structural fluctuations in proteins on the nanosecond time scaleJoseph R. Lakowicz and Gregorio WeberCite this: Biochemistry 1973, 12, 21, 4171–4179Publication Date (Print):October 1, 1973Publication History Published online1 May 2002Published inissue 1 October 1973https://pubs.acs.org/doi/10.1021/bi00745a021https://doi.org/10.1021/bi00745a021research-articleACS PublicationsRequest reuse...
We introduce a methodology, fluorescence lifetime imaging (FLIM), in which the contrast depends on at each point two-dimensional image and not local concentration and/or intensity of fluorophore. used FLIM to create images NADH when free solution bound malate dehydrogenase. This represents challenging case for because decay times are just 0.4 1.0 ns states, respectively. In present apparatus, created from series phase-sensitive obtained with gain-modulated intensifier recorded charge-coupled...
We report the development of highly versatile fluorescent and photostable core−shell Ag@SiO2 nanocomposites, which allows researchers flexibility to incorporate just about any fluorophores outer silica shell by two simple methods. To show generality preparation technique, we have developed three different probes: an organic fluorophore (Rh800) a lanthanide probe doped (noncovalently linked) (Alexa 647) covalently linked shell. When compared control nanoparticles (nanobubbles), with silver...
The <i>Journal of Biomedical Optics</i> (JBO) is a Gold Open Access journal that publishes peer-reviewed papers on the use novel optical systems and techniques for improved health care biomedical research.
A series of fluorophores with single-exponential fluorescence decays in liquid solution at 20 °C were measured independently by nine laboratories using single-photon timing and multifrequency phase modulation fluorometry instruments lasers as excitation source. The dyes that can serve lifetime standards for time-domain frequency-domain measurements are all commercially available, photostable under the conditions measurements, soluble solvents spectroscopic quality (methanol, cyclohexane,...
We prepared silver particle dimers with single Cy5 molecules localized between coupled metal particles. The particles a 20 nm diameter were chemically bound single-stranded oligonucleotides. formed by hybridization double-length oligonucleotides that contained molecules. image analysis revealed the single-molecule fluorescence was enhanced 7-fold on monomer and 13-fold dimer relative to free Cy5-labeled oligonucleotide in absence of metal. lifetimes shortened monomers further dimers,...
Three water-soluble fluorescent probes have been specifically designed to determine free cyanide concentrations up physiologically lethal levels, >20 microM. The in such a way as afford many notable sensing features, which render them unique with regard signal transduction, photophysical characteristics, and their application physiological determination safeguard. are readily able reversibly bind aqueous dissociation constants around 4 microM3. Subsequent binding modulates the intramolecular...