A5051154783- Advanced Proteomics Techniques and Applications
- Tryptophan and brain disorders
- Mass Spectrometry Techniques and Applications
- Venomous Animal Envenomation and Studies
- Metabolomics and Mass Spectrometry Studies
- Mosquito-borne diseases and control
- Epigenetics and DNA Methylation
- Neuroscience and Neuropharmacology Research
- Marine Invertebrate Physiology and Ecology
- Cellular transport and secretion
- Antimicrobial Peptides and Activities
- Neurogenesis and neuroplasticity mechanisms
- Nicotinic Acetylcholine Receptors Study
- Marine Sponges and Natural Products
- Alzheimer's disease research and treatments
- Ion channel regulation and function
- Photosynthetic Processes and Mechanisms
- Photoreceptor and optogenetics research
- Diet and metabolism studies
- Beetle Biology and Toxicology Studies
- RNA Research and Splicing
- Algal biology and biofuel production
- Receptor Mechanisms and Signaling
- Microbial Metabolic Engineering and Bioproduction
- Biotin and Related Studies
Universidade Federal do Pará
2019-2025
Universidade Estadual de Campinas (UNICAMP)
2015-2022
Universidade Federal do Rio Grande do Norte
2022
KU Leuven
2012-2020
Universidade Federal de Minas Gerais
2010-2020
Universidade do Tocantins
2019
Universidade Federal do Tocantins
2018
Hospital de Clínicas da Unicamp
2016
Abstract Zika virus (ZIKV) has been associated with microcephaly and other brain abnormalities; however, the molecular consequences of ZIKV to human development are still not fully understood. Here we describe alterations in neurospheres derived from induced pluripotent stem (iPS) cells infected strain that is circulating Brazil. Combining proteomics mRNA transcriptional profiling, over 500 proteins genes Brazilian infection were found be differentially expressed. These provide an...
Sea anemones are known to contain a wide diversity of biologically active peptides, mostly unexplored according recent peptidomic and transcriptomic studies. In the present work, neurotoxic fractions from exudates Stichodactyla helianthus Bunodosoma granulifera were analyzed by reversed-phase chromatography mass spectrometry. The first peptide fingerprints these sea assessed, revealing largest number components (156) so far found in anemone species, as well richer B. relation S. helianthus....
Sea anemone (Cnidaria, Anthozoa) venom is an important source of bioactive compounds used as tools to study the pharmacology and structure-function voltage-gated K+ channels (KV). These neurotoxins can be divided into four different types, according their structure mode action. In this work, for first time, two toxins were purified from Bunodosoma caissarum population Saint Peter Paul Archipelago, Brazil. Sequence alignment phylogenetic analysis reveals that BcsTx1 BcsTx2 are newest members...
We designed a peptide, PnPP-19, comprising the potential active core of Phoneutria nigriventer native toxin PnTx2-6. investigated its role on erectile function, and toxicity immunogenicity.Erectile function was evaluated by intracavernous pressure-to-mean arterial pressure ratio during electrical field stimulation rat pelvic ganglia. Cavernous strips were contracted with phenylephrine relaxation induced or without PnPP-19 (10(-8) M). Activity sodium channels electrophysiological screening...
Separate lines of evidence have demonstrated the involvement N-methyl-D-aspartate (NMDA) receptor and oligodendrocyte dysfunctions in schizophrenia. Here, we carried out shotgun mass spectrometry proteome analysis oligodendrocytes treated with NMDA antagonist MK-801 to gain potential insights into these effects at molecular level. The treatment led alterations levels 68 proteins, which are associated seven distinct biological processes. Most proteins involved energy metabolism many been...
To date, several families of peptide toxins specifically interacting with ion channels in scorpion venom have been described. One these comprise (called KTxs), known to modulate potassium channels. Thus far, 202 KTxs reported, belonging subfamilies α, β, γ, κ, δ, and λ-KTxs). Here we report on a previously described orphan toxin from Tityus serrulatus venom, named Ts11. We carried out an in-depth structure-function analysis combining 3D structure elucidation Ts11 electrophysiological...
A new vasoactive cytolytic toxin, referred to as Sp-CTx, has been purified from the venom of scorpionfish Scorpaena plumieri by a combination gel filtration and anion exchange chromatographies. An estimation Sp-CTx native molecular mass, performed size exclusion chromatography, demonstrated that it is 121 kDa protein. Further physicochemical studies revealed its glycoproteic nature dimeric constitution, comprising subunits approximately 65 (MALDI-TOF-MS). Such protein proved possess potent...
Hippocampus is the brain area where aluminum (Al) accumulates in abundance and widely associated with learning memory. In present study, we evaluate behavioral, tissue, proteomic changes hippocampus of Wistar rats caused by exposure to doses that mimic human consumption chloride (AlCl3) urban areas. For this, male were divided into two groups: Control (distilled water) AlCl3 (8.3 mg/kg/day), both groups exposed orally for 60 days. After Al protocol, cognitive functions assessed Water maze...
Schizophrenia is a psychiatric disorder that affects more than 21 million people worldwide. It an incurable and the primary means of managing symptoms through administration pharmacological treatments, which consist heavily antipsychotics. First-generation antipsychotics have properties D2 receptor antagonists. Second-generation antipsychotic are antagonists both 5HT2 receptors. Recently, there has been increasing interest in effects beyond their neuronal targets oligodendrocytes one main...
Abstract Oligodendrocytes are a type of neuroglia that provide trophic support and insulation to axons in the central nervous system. The genesis maturation oligodendrocytes essential processes for myelination course CNS development. Using ion mobility‐enhanced, data‐independent acquisitions 2D‐nanoUPLC fractionation operating at nanoscale flow rates, we established comprehensive data set proteins expressed by human oligodendroglia cell line MO3.13. final dataset incorporating all fractions...