A5069185283- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Vector-Borne Animal Diseases
- Insect symbiosis and bacterial influences
- Virology and Viral Diseases
- Protein Structure and Dynamics
- Aquaculture disease management and microbiota
- Evolution and Genetic Dynamics
- Glycosylation and Glycoproteins Research
- Algal biology and biofuel production
- Protist diversity and phylogeny
- Microbial Natural Products and Biosynthesis
- Fish biology, ecology, and behavior
- Vibrio bacteria research studies
- Viral gastroenteritis research and epidemiology
- Bacterial Genetics and Biotechnology
- Malaria Research and Control
- Animal Virus Infections Studies
- Aquatic Ecosystems and Phytoplankton Dynamics
- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- Viral Infections and Immunology Research
- HIV Research and Treatment
Instituto Evandro Chagas
2014-2022
Ministério da Saúde
2014-2021
Universidade Federal do Rio de Janeiro
2009-2012
Zika virus genomes from Brazil The outbreak is a major cause for concern in Brazil, where it has been linked with increased reports of otherwise rare birth defects and neuropathology. In phylogenetic analysis, Faria et al. infer single introduction to the Americas estimated date be about May December 2013—some 12 months earlier than was reported. This timing correlates events Brazilian cultural calendar associated traveler numbers areas circulating. A correlation also observed between...
In December 2013, an outbreak of Chikungunya virus (CHIKV) caused by the Asian genotype was notified in Caribbean. The has since spread to 38 regions Americas. By September 2014, first autochthonous CHIKV infections were confirmed Oiapoque, North Brazil, and Feira de Santana, Northeast Brazil. We compiled epidemiological clinical data on suspected cases Brazil polymerase-chain-reaction-based diagnostic conducted 68 serum samples from patients with symptom onset between April 2014. Two...
Abstract Zika virus (ZIKV) has been associated with microcephaly and other brain abnormalities; however, the molecular consequences of ZIKV to human development are still not fully understood. Here we describe alterations in neurospheres derived from induced pluripotent stem (iPS) cells infected strain that is circulating Brazil. Combining proteomics mRNA transcriptional profiling, over 500 proteins genes Brazilian infection were found be differentially expressed. These provide an...
Zika virus (ZIKV) infection during pregnancy is associated with a spectrum of developmental impairments known as congenital syndrome (CZS). The prevalence this varies across ZIKV endemic regions, suggesting that its occurrence could depend on cofactors. Here, we evaluate the relevance protein malnutrition for emergence CZS. Epidemiological data from outbreak in Americas suggest relationship between undernutrition and cases microcephaly. To experimentally examine relationship, use...
Lectins that bind to HIV envelope glycoprotein can inhibit virus-cell fusion and be used for rational drug design. This paper presents the results of an in silico approach improve affinity interaction between cyanobacterial lectin microvirin its ligand Manα(1-2)Man. Comparative modeling molecular dynamics tools were used. Additionally, alanine scanning webserver was study importance protein residues binding site guide mutant production. The model obtained presented two homologous domains...
Abstract Background Chikungunya virus (CHIKV) emerged in the Americas 2013 and has caused approximately 2.1 million cases >600 deaths. A retrospective investigation was undertaken to describe clinical, epidemiological, viral genomic features associated with deaths by CHIKV Ceará state, northeast Brazil. Methods Sera, cerebrospinal fluid (CSF), tissue samples from 100 fatal suspected arbovirus infection were tested for CHIKV, dengue (DENV), Zika (ZIKV). Clinical, death reports obtained...
Oropouche virus (OROV), genus Orthobunyavirus , family Bunyaviridae is an important cause of human illness in tropical South America. Herein, we report the isolation, complete genome sequence, genetic characterization, and phylogenetic analysis OROV species reassortant, Madre de Dios (MDDV), obtained from a sick monkey ( Cebus olivaceus Schomburgk) collected forest near Atapirire, small rural village located Anzoategui State, Venezuela. MDDV one growing number naturally occurring...
Tyrosinases belong to the functional copper-containing proteins family, and their structure contains two copper atoms, in active site, which are coordinated by three histidine residues. The biosynthesis of melanin melanocytes has stages depending on actions natural substrates L-DOPA L-tyrosine. dysregulation tyrosinase is involved skin cancer initiation. In present study, using molecular modeling tools, we analyzed inhibition activity kojic acid (KA) derivatives designed from aromatic...
Yellow Fever virus (YFV) is an important human pathogen in tropical areas of Africa and South America. Although efficient vaccine available has been used since the early 1940s, sylvatic YFV transmission still occurs forested where anthropogenic actions are present, such as mineral extraction, rearing livestock agriculture, ecological tourism. In this context, two distinct techniques based on RT-PCR derived method have previously developed, however both methods expensive due to use thermo...
Abstract The envelope (E) protein is an important target for antibodies in flavivirus. Literature reports that the mutation T198F, located at domain I-II hinge of E protein, regulates viral breathing and increases accessibility a distal cryptic epitope on fusion loop, having direct impact neutralization West Nile virus (WNV). Our study aimed to describe, using accelerated molecular dynamics simulations, effects T198F flexibility WNV elucidate mechanism accessibility. simulation results...
The complete genome was determined for 12 viruses isolated from 8 different pools of mosquitoes (Culex sp. and Psorophora ferox) collected at Brejeira farm, Canaan dos Carajas, Para state in northern Brazil. Eight the were distantly related to Piura virus, hereafter designated as virus; other 4 similar Wallerfield virus.
Differential phenotypic characteristics for taxonomic diagnosis purposes are well determined in the genus Saimiri (squirrel monkey). However, data on its reproductive lacking. Our aim was to determine testicular biometry and correlate with seminal analysis captive (Saimiri collinsi) free living vanzolinii, cassiquiarensis, macrodon) squirrel monkeys. Testicular length, width, height, circumference, volume were measured. showed no differences between right left testicles within same species,...
Background The Arapaima (Arapaima gigas) is one of the world's largest freshwater bony fish, and found in rivers Amazon basin. This species a potential aquaculture resource, although reproductive management captivity limited particular due to lack external sexual dimorphism. In this study, using 454 Roche platform (pyrosequencing) techniques, we evaluated major portion transcriptome important Amazonian species. Results Four libraries obtained from liver skin tissue juvenile specimens...
The polymorphism of species A rotavirus genotype G1 strains (RVA-G1) circulating in Rio de Janeiro between 1996 and 2004 was evaluated. VP7 encoding gene 36 isolates sequenced compared to references strains. deduced amino acid sequences were used as basis for silico analysis the protein. We observed circulation two major lineages five sublineages during studied period. Comparison trimeric structures a vaccine strain Brazilian showed mutations at residues located calcium binding site several...
Nearly complete genome sequences for three ungrouped viruses, Pacui virus (BEAN27326), Rio Preto da Eva (BEAR540870), and Tapirape (BEAN767592) isolated in the Amazon region are reported here. All genomic segments (small, medium large RNA) were recovered similar to members of genus Orthobunyavirus.
Background The C. macropomum is a characiform fish from the Amazon basin that has been hybridized with other pacu species to produce commercial hybrids, such as tambacu. However, little known of functional genomics parental or these hybrid forms. transcriptome and tambacu were sequenced using 454 Roche platform (pyrosequencing) techniques characterize domains Gene Ontology (GO) evaluate levels gene expression in two organisms. Results 8,188,945 reads assembled into 400,845 contigs. A total...
The genus Orbivirus of the family Reoviridae comprises 22 virus species including Changuinola (CGLV) serogroup. complete genome sequences 13 CGLV serotypes isolated between 1961 and 1988 from distinct geographical areas Brazilian Amazon region were obtained. All viral obtained single-passaged strains grown in Vero cells. CGLVs are only orbiviruses known to be transmitted by phlebotomine sandflies. Ultrastructure molecular analysis electron microscopy gel electrophoresis, respectively,...
Haemagogus janthinomys is a mosquito of high importance in public health due its involvement on natural wild cycles two important arboviruses the Brazilian Amazon region: Yellow Fever virus (Flaviviridae, Flavivirus) and Mayaro (Togaviridae, Alphavirus). Here, we have sequenced described all mitochondrial genes for Hg. species. The complete coding sequence is14 937 bp long includes 37 functional genes, which 13 codes proteins, 22 tRNA 2 ribosomal subunits. Region A + T (control region) not...
Crithidia deanei is a trypanosomatid protozoan that harbours symbiotic bacterium. The partners maintain mutualistic relationship, thus constituting an excellent model for studying metabolic exchanges between the host and symbiont, origin of organelles cellular evolution. According to molecular analysis, symbionts different species share high identity descend from common ancestor, β-proteobacterium genus Bordetella. endosymbiont surrounded by two membranes, like Gram-negative bacteria, but...
Given the scarcity of data pertaining to whole-genome sequences cyanobacterial strains isolated in Brazil, we hereby present draft genome sequence Cyanobium sp. strain CACIAM 14, southeastern Amazonia.
Abstract Lectins are proteins of nonimmune origin, which capable recognizing and binding to glycoconjugate moieties. Some them can block the interaction viral glycoproteins host cell receptors acting as antiviral agents. Although cyanobacterial lectins have presented broad biotechnological potential, little research has been directed Amazonian Cyanobacterial diversity. In order identify new lectins, we performed genomic analysis in seven strains from Coleção Amazônica de Cianobactérias e...
Chagas disease, caused by Trypanosoma cruzi, is one of the neglected parasitic tropical diseases affecting thirteen million people annually. At present, there are only two compounds used in clinical treatment to this disease and they were introduced 1960s 1970s. Nifurtimox Benznidazole have limited effectiveness serious side effects. New strategies targets for an effective chemotherapy American trypanosomiasis need be developed. Within framework, peptidases aroused great interest as a...