- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Malaria Research and Control
- Insect symbiosis and bacterial influences
- Vector-Borne Animal Diseases
- Vector-borne infectious diseases
- Zoonotic diseases and public health
- Insect Pest Control Strategies
- Viral Infections and Outbreaks Research
- Plant Virus Research Studies
- Insect and Pesticide Research
- Vibrio bacteria research studies
- Animal Disease Management and Epidemiology
- Insect behavior and control techniques
- Dengue and Mosquito Control Research
- Genomics and Phylogenetic Studies
- Insect-Plant Interactions and Control
- Insect Resistance and Genetics
- Toxoplasma gondii Research Studies
- Aquaculture disease management and microbiota
Ministério da Saúde
2008-2025
Instituto Evandro Chagas
2013-2025
Universidade do Estado do Pará
2009-2025
Ministry of Health of the Russian Federation
2023
Secretaria da Saúde
2010
Universidade de São Paulo
2010
Abstract In February 2008, a Mayaro fever virus (MAYV) outbreak occurred in settlement Santa Barbara municipality, northern Brazil. Patients had rash, fever, and severe arthralgia lasting up to 7 days. Immunoglobulin M against MAYV was detected by ELISA 36 persons; 3 isolates sequenced were characterized as genotype D.
Abstract Yellow fever virus (YFV) was isolated from Haemagogus leucocelaenus mosquitoes during an epizootic in 2001 the Rio Grande do Sul State southern Brazil. In October 2008, a yellow outbreak reported there, with nonhuman primate deaths and human cases. This latter led to intensification of surveillance measures for early detection YFV support vaccination programs. We report entomologic 2 municipalities that recorded deaths. Mosquitoes were collected at ground level, identified,...
A wide diversity of pathogenic mosquito-borne viruses circulate in the Brazilian Amazon, and intense deforestation can contribute to spread these viruses. In this context, study aimed investigate viral mosquitoes genera Aedes, Culex, Haemagogus, Sabethes from a transition area between Cerrado, Caatinga biomes Brazil. Metagenomic high-throughput sequencing was used characterize virome 20 mosquito pools. total 15 virus-like genomes were identified, comprising species genomically close...
The Quilombola community of Abacatal, located in Ananindeua, the state Pará, has characteristics that favor proliferation mosquitoes. Faunal surveys environmental preservation areas are essential for understanding dynamics these vectors, whose epidemiological implications significant. Uncontrolled human activities have an impact on temperature, humidity, and rainfall. aim this study was to survey diversity mosquito species Abacatal. Field collections were carried out over 10 days, during...
The genus Phlebovirus of the family Bunyaviridae consists approximately 70 named viruses, currently assigned to nine serocomplexes (species) based on antigenic similarities. Sixteen other viruses that show little serologic relationship recognized groups are also classified as tentative species in genus. In an effort develop a more precise classification system for phleboviruses, we attempting sequence most with goal clarifying their phylogenetic relationships. this report, describe and...
West Nile virus is characterized as a neurotropic pathogen, which can cause fever and transmitted by mosquitoes of the genus Culex. In 2018, Instituto Evandro Chagas performed first isolation WNV strain in Brazil from horse brain sample. The present study aimed to evaluate susceptibility orally infected Cx. quinquefasciatus Amazon region become transmit isolated 2018. Oral infection was with blood meal artificially WNV, followed analysis infection, dissemination, transmission rates, well...
Mosquitoes as Sabethes chloropterus, glaucodaemon, belisarioi are species of medical and epidemiological importance for arboviruses transmission such yellow fever St. Louis encephalitis. Despite this, no information about these three mitochondrial DNA has been found in literature. Our study presents a genome description, including identity, SNPs, mutation rate, phylogeny analysis using COX1, COX2, NADH4, NADH5, CYOB genes. The Sa. glaucodaemon belisaroi sizes 15.609 bp, 15.620 15.907...
West Nile virus is a flavivirus transmitted by mosquitoes, mainly of the genus Culex. In Brazil, serological studies have already indicated circulation since 2003, with first human case detected in 2014. The objective present paper to report isolation WNV Culex (Melanoconion) mosquito. Arthropods were collected protected attraction and CDC light bait, taxonomically identified analyzed viral isolation, complement fixation genomic sequencing tests. was isolated from samples analysis...
The risk of the emergence and reemergence zoonoses is high in regions that are under strong influence anthropogenic actions, as they contribute to vector disease transmission. Yellow fever (YF) among main pathogenic arboviral diseases world, Culicidae Aedes albopictus has been proposed having potential transmit yellow virus (YFV). This mosquito inhabits both urban wild environments, experimental conditions, it shown be susceptible infection by YFV. In this study, competence Ae. for YFV was...
Because of its ecological characteristics, the Caxiuanã National Forest (FLONA) is a potential area arbovirus circulation. The present study aimed to investigate occurrence transmission cycles at FLONA de Caxiuanã. Five field trips were performed capture mosquitoes and sylvatic vertebrates. For these vertebrates, we attempted viral isolation by cell monolayer inoculation from blood, hemagglutination inhibition further seroneutralization assays sera. mosquitoes, tests genome detection. A...
The genus Orthopodomyia Theobald, 1904 (Diptera: Culicidae) comprises 36 wild mosquito species, with distribution largely restricted to tropical and temperate areas, most of which are not recognized as vectors epidemiological importance due the lack information related their bionomy involvement in cycle transmission infectious agents. Furthermore, evolutionary relationships completely understood, reflecting scarcity genetic about genus. Therefore, this study, we report first complete...
Abstract The genus Haemagogus (Diptera: Culicidae) comprises species of great epidemiological relevance, involved in transmission cycles the Yellow fever virus and other arboviruses South America. So far, only janthinomys has complete mitochondrial sequences available. Given unavailability information related to aspects evolutionary biology molecular taxonomy this genus, we report here, first sequencing mitogenomes albomaculatus , leucocelaenus spegazzinii tropicalis . showed an average...
The risk of the emergence and reemergence zoonoses is high in regions that are under anthropogenic actions, as they contribute to vector disease transmission. Yellow fever (YF) among main pathogenic arboviral world, Culicidae Aedes albopictus has been proposed have potential transmit yellow virus (YFV). This mosquito inhabits both urban wild environments, experimental conditions, it shown be susceptible infection by YFV. In this study, competence Ae. for YFV was investigated. Female were...
Viruses with encephalitogenic potential can cause neurological conditions of clinical and epidemiological importance, such as Saint Louis encephalitis virus, Venezuelan equine Eastern Western Dengue Zika Chikungunya Mayaro virus West Nile virus. The objective the present study was to determine number arboviruses neuroinvasive isolated in Brazil that corresponds collection viral samples belonging Department Arbovirology Hemorrhagic Fevers, Evandro Chagas Institute (SAARB/IEC) Laboratory...
O presente estudo descreve os aspectos eco-epidemiológicos sobre arbovírus nos Municípios de Novo Progresso e Trairão, Estado do Pará, Brasil, na área influência da BR 163. Anticorpos IH foram detectados para diferentes arbovírus, com reações monotípicas VMAY VORO, dois importantes associados a epidemias Amazônia. IgM o VORO em soros humanos, sugerindo infecção recente por esses arbovírus. Duas cepas VDEN-3 isoladas pacientes febris residentes identificadas como genótipo III. Em termos...
Dengue fever, chikungunya, and Zika are diseases caused by viruses transmitted Aedes aegypti albopictus. In Brazil, the number of human infections is high, but few studies performed in mosquito vectors. This study aimed to investigate presence Zika, Chikungunya Ae. albopictus from municipalities Alto Alegre, Caxias, Codó, São Mateus do Maranhão, located state Northeast Brazil. The mosquitoes were collected with a mechanical aspirator, identified, triturated, then submitted RNA extraction...
O objetivo foi avaliar a prevalência de anticorpos para arbovírus na área influência do Projeto Juruti antes da exploração mineral local. Foram examinados 1.597 soros humanos e 85 animais silvestres. A pesquisa realizada pelo teste inibição hemaglutinação contra antígenos dos mais prevalentes Amazônia brasileira IgM-ELISA dengue febre amarela. Soros inibidores positivos Mayaro Oropouche foram também testados por IgM-ELISA. Anticorpos alfavírus, orthobunyavirus e/ou flavivírus detectados,...
The complete genome was determined for 12 viruses isolated from 8 different pools of mosquitoes (Culex sp. and Psorophora ferox) collected at Brejeira farm, Canaan dos Carajas, Para state in northern Brazil. Eight the were distantly related to Piura virus, hereafter designated as virus; other 4 similar Wallerfield virus.
Chikungunya virus (CHIKV) was first reported in Brazil 2014 and, after it spread countrywide, an outbreak of febrile illness with reports arthralgia happened the municipality Xinguara, Pará, 2017, indicating virus' circulation. Here, we aimed to investigate CHIKV mosquito vectors collected during active surveillance isolation cell culture by using molecular detection and viral genome sequencing. A total 492 Aedes, Culex Mansonia mosquitoes were separated 36 pools according species sex, 22.2%...
The Culicidae family is distributed worldwide and comprises about 3587 species subdivided into the subfamilies Anophelinae Culicinae. This first description of complete mitochondrial DNA sequences from Aedes fluviatilis, Aedeomyia squamipennis, Coquillettidia nigricans, Psorophora albipes, ferox. mitogenomes showed an average length 15,046 pb 78.02% AT content, comprising 37 functional subunits (13 protein coding genes, 22 tRNAs, two rRNAs). most common start codons were ATT/ATG, TAA was...