A5018527455- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- SARS-CoV-2 and COVID-19 Research
- Mosquito-borne diseases and control
- Pharmacological Receptor Mechanisms and Effects
- Neurogenesis and neuroplasticity mechanisms
- Axon Guidance and Neuronal Signaling
- Receptor Mechanisms and Signaling
- Tryptophan and brain disorders
- Parkinson's Disease Mechanisms and Treatments
- Alzheimer's disease research and treatments
- Planarian Biology and Electrostimulation
- Barrier Structure and Function Studies
- Epigenetics and DNA Methylation
- Neuroinflammation and Neurodegeneration Mechanisms
- Infectious Encephalopathies and Encephalitis
- Cannabis and Cannabinoid Research
- Ferroptosis and cancer prognosis
- Nerve injury and regeneration
- Ion Channels and Receptors
- Hepatitis C virus research
- 3D Printing in Biomedical Research
- Fatty Acid Research and Health
- Pluripotent Stem Cells Research
- Peroxisome Proliferator-Activated Receptors
D’Or Institute for Research and Education
2016-2024
Universidade Federal do Rio de Janeiro
2021
Abstract Zika virus (ZIKV) has been associated with microcephaly and other brain abnormalities; however, the molecular consequences of ZIKV to human development are still not fully understood. Here we describe alterations in neurospheres derived from induced pluripotent stem (iPS) cells infected strain that is circulating Brazil. Combining proteomics mRNA transcriptional profiling, over 500 proteins genes Brazilian infection were found be differentially expressed. These provide an...
Abstract Background Current approaches of drug repurposing against COVID-19 have not proven overwhelmingly successful and the SARS-CoV-2 pandemic continues to cause major global mortality. nsp12, its RNA polymerase, shares homology in nucleotide uptake channel with HCV orthologue enzyme NS5B. Besides, NS5A has pleiotropic activities, such as binding, that are shared various proteins. Thus, anti-HCV NS5B inhibitors, like sofosbuvir daclatasvir, respectively, could be endowed anti-SARS-CoV-2...
Abstract Schizophrenia is a neurodevelopmental disease characterized by cerebral connectivity impairment and loss of gray matter. It was described in adult schizophrenia patients (SZP) that concentration VEGFA, master angiogenic factor, decreased. Recent evidence suggests hypoperfusion related to dysfunctional Blood Brain Barrier (BBB) SZP. Since neurogenesis blood-vessel formation occur coincident coordinated fashion, defect neurovascular development could result increased vascular...
Neural crest stem cells (NCPCs) have been shown to differentiate into various cell types and tissues during embryonic development, including sensory neurons. The few studies addressing the generation of NCPCs peripheral neurons (PSNs) from human induced pluripotent (hiPSCs), generated without displaying robust activity. Here, we describe an efficient strategy for hiPSCs differentiation functional PSNs using chemically defined media factors achieve differentiation, confirmed by expression...
Abstract Current approaches of drugs repurposing against 2019 coronavirus disease (COVID-19) have not proven overwhelmingly successful and the severe acute respiratory syndrome 2 (SARS-CoV-2) pandemic continues to cause major global mortality. Daclatasvir (DCV) sofosbuvir (SFV) are clinically approved hepatitis C virus (HCV), with satisfactory safety profile. DCV SFV target HCV enzymes NS5A NS5B, respectively. is endowed pleotropic activities, which overlap several proteins from SARS-CoV-2....
Coronavirus disease 2019 (COVID-19) was initially described as a viral infection of the respiratory tract. It is now known, however, that several other organs are affected, including brain. Neurological manifestations such stroke, encephalitis, and psychiatric conditions have been reported in COVID-19 patients, but neurotropic potential virus still debated. Herein, we sought to investigate SARS-CoV-2 human neural cells. We demonstrated tissue non-permissive, it can elicit inflammatory...
Age increases the risk for cognitive impairment and is single major factor Alzheimer's disease (AD), most prevalent form of dementia in elderly. The pathophysiological processes triggered by aging that render brain vulnerable to involve, at least part, changes inflammatory mediators. Here we show lipoxin A4 (LXA4), a lipid mediator inflammation resolution known stimulate endocannabinoid signaling brain, reduced central nervous system. We demonstrate genetic suppression 5-lipoxygenase...
Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which can infect several organs, especially impacting capacity. Among the extrapulmonary manifestations of COVID-19 myocardial injury, associated with a high risk mortality. Myocardial directly or indirectly SARS-CoV-2 infection, be triggered inflammatory processes that lead to damage heart tissue. Since one hallmarks “cytokine storm”, strategies control inflammation infection have...
SARS-CoV-2 infects cardiac cells and causes heart dysfunction. Conditions such as myocarditis arrhythmia have been reported in COVID-19 patients. The Sigma-1 receptor (S1R) is a ubiquitously expressed chaperone that plays central role cardiomyocyte function. S1R has proposed therapeutic target because it may affect replication; however, the impact of inhibition human cardiomyocytes remains to be described. In this study, we investigated consequences iPSC-derived (hiPSC-CM). infection...
Zika virus (ZIKV) has been associated with microcephaly and other brain abnormalities; however, the molecular cellular consequences of circulating in Brazil to human development have not studied yet. Here we describe alterations neurospheres derived from neural stem cells infected Brazilian ZIKV. Combined proteomics mRNA transcriptional profile analyses showed that ZIKV, prior induce cell death, alters cycle halts neurogenic programmes, addition regulate transcription protein translation due...
Abstract Coronavirus disease 2019 (COVID-19) was initially described as a viral infection of the respiratory tract. It is now known, however, that several other organs are affected, including brain. Neurological manifestations such stroke, encephalitis, and psychiatric conditions have been reported in COVID-19 patients, but neurotropic potential virus still debated. Herein, we sought to investigate SARS-CoV-2 human neural cells. We demonstrated tissue non-permissive, it can elicit...
ABSTRACT Heart dysfunction, represented by conditions such as myocarditis and arrhythmia, has been reported in COVID-19 patients. Therapeutic strategies focused on the cardiovascular system, however, remain scarce. The Sigma-1 receptor (S1R) recently proposed a therapeutic target because its inhibition reduces SARS-CoV-2 replication. To investigate role of S1R infection heart, we used human cardiomyocytes derived from induced pluripotent stem cells (hiPSC-CM) an experimental model. Here show...
Aged and photoaged skin exhibit fine wrinkles that are signs of epidermal inflammation degeneration. It has been shown healthy elderly expresses amyloidogenic proteins, including α-Synuclein, which known to oligomerize trigger neurodegeneration. However, little is about their putative role in physiology sensitivity. To unravel this possible role, we investigated the impact oligomeric α-Synuclein (Oα-Syn) 2D 3D keratinocyte human models. Exogenous Oα-Syn caused degeneration reconstructed...
Abstract Neural crest stem cells (NCPCs) have been shown to differentiate into various cell types and tissues during embryonic development, including sensory neurons. The few studies addressing the generation of NCPCs peripheral neurons (PSNs) from human induced Pluripotent Stem Cells (hiPSCs), generated without displaying robust activity. Here, we describe an efficient strategy for hiPSCs differentiation functional PSNs using chemically defined media factors achieve differentiation,...
Zika virus (ZIKV) has been associated with microcephaly and other brain abnormalities; however, the molecular cellular consequences of circulating in Brazil to human development have not studied yet. Here we describe alterations neurospheres derived from neural stem cells infected Brazilian ZIKV. Combined proteomics mRNA transcriptional profile analyses showed that ZIKV, prior induce cell death, alters cycle halts neurogenic programmes, addition regulate transcription protein translation due...
Neuroinflammation, particularly astrocyte reactivity, are increasingly linked to schizophrenia (SCZ). Yet, the crosstalk between astrocytes and microglia in SCZ, especially under pro-inflammatory conditions, remains unclear. Here, we apply human induced pluripotent stem cells (hiPSCs) compare reactivity of from individuals with SCZ neurotypical controls, towards resolving how patient-derived affect microglial biology. TNF-ɑ stimulation astrocytes, relative control trigger an increase...
ABSTRACT The Zika virus (ZIKV) outbreak in Brazil between 2015 and 2016 was associated with an increased prevalence of severe congenital malformations, including microcephaly. Notably, the distribution microcephaly cases not uniform across country, a disproportionately higher incidence recorded Northeast region (NE). Our previous work demonstrated that saxitoxin (STX), toxin ubiquitously present drinking water reservoirs NE, exacerbated damaging effects ZIKV on developing brain. In study, we...
Abstract Schizophrenia (SZ) is a complex neuropsychiatric disorder, affecting 1% of the world population. Long-standing clinical observations and molecular data have pointed out possible vascular deficiency that could be acting synergistically with neuronal dysfunction in SZ. As SZ neurodevelopmental disease, use human induced pluripotent stem cells (hiPSC) allows disease biology modeling retaining patient’s unique genetic signature. Previously, we reported VEGF-A signaling impairment...
Age increases the risk for cognitive impairment and is single major factor Alzheimer's disease (AD), most prevalent form of dementia in elderly. The pathophysiological processes triggered by aging that render brain vulnerable to involve, at least part, changes inflammatory mediators. Here we show lipoxin A4 (LXA4), a lipid mediator inflammation resolution known stimulate endocannabinoid signaling brain, reduced central nervous system. We demonstrate genetic suppression 5-lipoxygenase...