A5090149132- Pluripotent Stem Cells Research
- Reproductive Biology and Fertility
- Single-cell and spatial transcriptomics
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- bioluminescence and chemiluminescence research
- Ovarian function and disorders
- Cell Image Analysis Techniques
- Tissue Engineering and Regenerative Medicine
- Neuroethics, Human Enhancement, Biomedical Innovations
- 3D Printing in Biomedical Research
- Reproductive Health and Technologies
- Bioinformatics and Genomic Networks
- Fatty Acid Research and Health
- Neurological disorders and treatments
- Epigenetics and DNA Methylation
- Extracellular vesicles in disease
- Neurogenesis and neuroplasticity mechanisms
- Gene Regulatory Network Analysis
- Renal and related cancers
- Biomedical Ethics and Regulation
- Cholesterol and Lipid Metabolism
- Genetic Syndromes and Imprinting
- Ferroptosis and cancer prognosis
- Animal Genetics and Reproduction
GameDesk
2023-2024
Harvard University Press
2019-2024
Broad Institute
2020-2023
Harvard University
2019-2023
College of New Rochelle
2023
Universidade Federal do Rio de Janeiro
2011-2016
D’Or Institute for Research and Education
2014
Schizophrenia has been defined as a neurodevelopmental disease that causes changes in the process of thoughts, perceptions, and emotions, usually leading to mental deterioration affective blunting. Studies have shown altered cell respiration oxidative stress response schizophrenia; however, most knowledge acquired from postmortem brain analyses or nonneural cells. Here we describe neural cells, derived induced pluripotent stem cells generated skin fibroblasts schizophrenic patient, presented...
Schizophrenia is a neurodevelopmental disorder with genetic and environmental factors contributing to its pathogenesis, although the mechanism unknown due difficulties in accessing diseased tissue during human neurodevelopment. The aim of this study was find neuronal differentiation genes disrupted schizophrenia evaluate those post-mortem brain tissues from cases controls. We analyzed differentially expressed (DEG), copy number variation (CNV) differential methylation induced pluripotent...
Abstract STUDY QUESTION Can in vitro maturation (IVM) and developmental competence of human oocytes be improved by co-culture with ovarian support cells (OSCs) derived from human-induced pluripotent stem (hiPSCs)? SUMMARY ANSWER OSC-IVM significantly improves the rates metaphase II (MII) formation euploid Day 5 or 6 blastocyst formation, when compared to a commercially available IVM system. WHAT IS KNOWN ALREADY has historically shown highly variable performance maturing generating strong...
Glioblastoma (GBM) is characterized by heterogeneous malignant cells that are functionally integrated within the neuroglial microenvironment. In this study, we model ecosystem growing GBM into long-term cultured human cortical organoids contain major cell types found in cerebral cortex. Single-cell RNA sequencing analysis suggests that, compared with matched gliomasphere models, more faithfully recapitulate diversity and expression programs of states patient tumors. Additionally, observe...
Abstract Realizing the full utility of brain organoids as experimental systems to study human cortical development requires understanding whether replicate cellular and molecular events this complex process precisely, reproducibly, with fidelity embryo. Here we present a comprehensive single-cell transcriptomic, epigenetic, spatial atlas organoid development, comprising over 610,000 cells, spanning initial generation neural progenitors through production differentiated neuronal glial...
De novo heterozygous loss-of-function mutations in phosphatase and tensin homolog (PTEN) are strongly associated with autism spectrum disorders; however, it is unclear how this gene affect different cell types during human brain development these effects vary across individuals. Here, we used cortical organoids from donors to identify cell-type specific developmental events that affected by PTEN. We profiled individual single-cell RNA-seq, proteomics spatial transcriptomics revealed...
<p>Supplementary Figure S5 describes extracellular vesicle mediated intercellular transfer in GCOs, related to 5.</p>
<p>Supplementary Figure S3 describes GFP transfer process in GCOs, related to 3.</p>
<p>Text file shows the GFP sequence from pLV-CMV-GFP used to align reads scRNA-seq data.</p>
<p>Supplementary Figure S4 describes widespread transfer of endogenous malignant transcripts in GCOs, related to 4.</p>
<p>Supplementary Figure S2 describes malignant cell states in GCOs, related to 2.</p>
<p>Supplementary Figure S1 describes the establishment and scRNA-seq profiling of GCOs, related to 1.</p>
<div>Abstract<p>Glioblastoma (GBM) is characterized by heterogeneous malignant cells that are functionally integrated within the neuroglial microenvironment. In this study, we model ecosystem growing GBM into long-term cultured human cortical organoids contain major cell types found in cerebral cortex. Single-cell RNA sequencing analysis suggests that, compared with matched gliomasphere models, more faithfully recapitulate diversity and expression programs of states patient...
Human induced pluripotent stem cells (hiPSCs) show great promise in the development of novel strategies to mitigate reproductive diseases and promote successful outcomes. Recently, a approach for fast efficient differentiation ovarian support (OSCs) generate versatile platform basic research clinical applications was demonstrated. This study details process application an OSC product, known as Fertilo, improve vitro maturation (IVM) human oocytes, method referred OSC-IVM. First,...
(Abstracted from J Assist Reprod Genet 2024;41(8):2021–2036) Embryo development is orchestrated by several somatic types of ovarian cells, including stroma, theca, and granulosa cells that each send signals to oocytes; during oocyte maturation, oocytes rely on posttranscriptional modification, transcript degradation, selective translation. There little literature date has focused transcriptome-wide changes in gene expression after vitro maturation (IVM), although the mechanisms may differ...