A5081802188- Single-cell and spatial transcriptomics
- CRISPR and Genetic Engineering
- Genetics, Aging, and Longevity in Model Organisms
- RNA Research and Splicing
- Extracellular vesicles in disease
- Circadian rhythm and melatonin
- Lymphoma Diagnosis and Treatment
- RNA regulation and disease
- Cancer-related molecular mechanisms research
- Microbial Community Ecology and Physiology
- Autism Spectrum Disorder Research
- Long-Term Effects of COVID-19
- Gene Regulatory Network Analysis
- Glioma Diagnosis and Treatment
- Immune cells in cancer
- RNA modifications and cancer
- Cannabis and Cannabinoid Research
- MicroRNA in disease regulation
- COVID-19 and Mental Health
- Cell Image Analysis Techniques
- Ferroptosis and cancer prognosis
- Cancer, Stress, Anesthesia, and Immune Response
- Circular RNAs in diseases
- Lipid metabolism and disorders
- RNA Interference and Gene Delivery
Scripps Health
2023-2025
Scripps Research Institute
2009-2025
BGI Group (China)
2012-2025
South China University of Technology
2012-2025
Harvard University
2018-2024
Broad Institute
2016-2024
East China Normal University
2024
New York University Shanghai
2024
Shaoxing University
2021-2024
BGI Research
2024
Delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana, and other direct cannabinoid receptor (CB1) agonists produce a number neurobehavioral effects in mammals that range from beneficial (analgesia) to untoward (abuse potential). Why, however, this full spectrum activities is not observed upon pharmacological inhibition or genetic deletion either fatty acid amide hydrolase (FAAH) monoacylglycerol lipase (MAGL), enzymes regulate two major endocannabinoids anandamide...
Eukaryotic genomes contain domesticated genes from integrating viruses and mobile genetic elements. Among these are homologs of the capsid protein (known as Gag) long terminal repeat (LTR) retrotransposons retroviruses. We identified several mammalian Gag that form virus-like particles one LTR retrotransposon homolog, PEG10, preferentially binds facilitates vesicular secretion its own messenger RNA (mRNA). showed mRNA cargo PEG10 can be reprogrammed by flanking interest with Peg10's...
Significance Genome editing technologies enable the permanent repair of disease-causing genetic mutations. However, application this technology has been limited by technical challenge achieving safe, effective, and specific in vivo delivery CRISPR-Cas9 genome components. Here, we report development a newly identified lipid nanoparticle (LNP) for mRNA to liver. While LNPs have FDA approved siRNA liver, here examine their editing. When compared head-to-head, our platform significantly...
The number of disease risk genes and loci identified through human genetic studies far outstrips the capacity to systematically study their functions. We applied a scalable screening approach, in vivo Perturb-Seq, functionally evaluate 35 autism spectrum disorder/neurodevelopmental delay (ASD/ND) de novo loss-of-function genes. Using CRISPR-Cas9, we introduced frameshift mutations these pools, within developing mouse brain utero, followed by single-cell RNA-sequencing perturbed cells...
Leveraging AAVs' versatile tropism and labeling capacity, we expanded the scale of in vivo CRISPR screening with single-cell transcriptomic phenotyping across embryonic to adult brains peripheral nervous systems. Through extensive tests 86 vectors AAV serotypes combined a transposon system, substantially amplified efficacy accelerated gene delivery from weeks days. Our proof-of-principle utero screen identified pleiotropic effects Foxg1, highlighting its tight regulation distinct networks...
Despite the widespread implementation of public health measures, coronavirus disease 2019 (COVID-19) continues to spread in United States. To facilitate an agile response pandemic, we developed How We Feel, a web and mobile application that collects longitudinal self-reported survey responses on health, behaviour demographics. Here, report results from over 500,000 users States 2 April 2020 12 May 2020. show surveys can be used build predictive models identify likely COVID-19-positive...
Objective To investigate the blood coagulation function in COVID-19 patients, and correlation between coagulopathy disease severity. Methods We retrospectively collected 147 clinically diagnosed patients at Wuhan Leishenshan Hospital of Hubei, China. analyzed through data including thrombin-antithrombin complex (TAT), α2-plasmininhibitor-plasmin Complex (PIC), thrombomodulin (TM), t-PA/PAI-1 (t-PAIC), prothrombin time (PT), international normalized ratio (INR), activated partial...
To gain insight into how researchers of aging perceive the process they study, we conducted a survey among experts in field. While highlighting some common features aging, exposed broad disagreement on foundational issues. What is aging? causes it? When does it begin? constitutes rejuvenation? Not only was there no consensus these and other core questions, but none questions received majority opinion-even regarding need for itself. Despite many believing understand their understanding...
Fatty acid amide hydrolase (FAAH) regulates amidated lipid transmitters, including the endocannabinoid anandamide and its N-acyl ethanolamine (NAE) congeners transient receptor potential channel agonists taurines (NATs). Using both FAAH inhibitor PF-3845 FAAH(-/-) mice, we present a global analysis of changes in NAE NAT metabolism caused by disruption central peripheral tissues. Elevations (and other NAEs) were tissue dependent, with most dramatic occurring brain, testis, liver...
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Abstract Aneuploidy and copy-number alterations (CNAs) are a hallmark of human cancer. Although genetically engineered mouse models (GEMMs) commonly used to model cancer, their chromosomal landscapes remain underexplored. Here we use gene expression profiles infer CNAs in 3,108 samples from 45 models, providing the first comprehensive catalogue aberrations cancer GEMMs. Mining this resource, find that most accumulate late during breast tumorigenesis, observe marked differences CNA prevalence...