A5043268929- Neuroendocrine regulation and behavior
- Connective tissue disorders research
- Neurotransmitter Receptor Influence on Behavior
- Tryptophan and brain disorders
- Receptor Mechanisms and Signaling
- Infant Health and Development
- Stress Responses and Cortisol
- Neuroscience and Neuropharmacology Research
- Bone and Dental Protein Studies
- Genetics and Neurodevelopmental Disorders
- Neuroscience and Neural Engineering
- Autism Spectrum Disorder Research
- Neuroscience of respiration and sleep
- Cancer-related gene regulation
- Child Development and Digital Technology
- Zebrafish Biomedical Research Applications
- Human-Animal Interaction Studies
- Protease and Inhibitor Mechanisms
- Tissue Engineering and Regenerative Medicine
- Evolutionary Psychology and Human Behavior
- Cannabis and Cannabinoid Research
- Cell Image Analysis Techniques
- Cognitive Science and Mapping
- Biochemical and biochemical processes
- Microtubule and mitosis dynamics
Weill Cornell Medicine
2023-2025
Cornell University
2023-2025
New York Proton Center
2025
Center for Autism and Related Disorders
2023-2025
Allen Institute for Brain Science
2023-2025
MIND Research Institute
2023-2024
Icahn School of Medicine at Mount Sinai
2018-2023
Heidelberg University
2022
Central Institute of Mental Health
2022
University Hospital Heidelberg
2022
Leveraging AAVs' versatile tropism and labeling capacity, we expanded the scale of in vivo CRISPR screening with single-cell transcriptomic phenotyping across embryonic to adult brains peripheral nervous systems. Through extensive tests 86 vectors AAV serotypes combined a transposon system, substantially amplified efficacy accelerated gene delivery from weeks days. Our proof-of-principle utero screen identified pleiotropic effects Foxg1, highlighting its tight regulation distinct networks...
Abstract Social behaviors are crucial for human connection and belonging, often impacted by conditions like Autism Spectrum Disorder (ASD). The mesoaccumbens pathway (ventral tegmental area (VTA) to the nucleus accumbense (NAc)) plays a pivotal role in social behavior is implicated ASD. However, impact of ASD‐related mutations on reward processing remains insufficiently explored. This study focuses Shank3 mutation, associated with rare genetic condition linked ASD, examining its influence...
Malignant gliomas are highly invasive tumors with an almost invariably rapid and lethal outcome. Surgery chemoradiotherapy fail to remove resistant tumor cells that disperse within normal tissue, which a major cause for disease progression therapy failure. Infiltration of the neural parenchyma is distinctive property malignant compared other solid tumors. Thus, glioma thought produce unique molecular changes remodel extracellular matrix form microenvironment permissive their motility. Here,...
Action potentials (APs) propagating along axons require the activation of voltage-gated Na+ (Nav) channels. How Nav channels are transported into is unknown. We show that KIF5/kinesin-1 directly binds to ankyrin-G (AnkG) transport axons. KIF5 and Nav1.2 bind multiple sites in AnkG N-terminal domain contains 24 ankyrin repeats. Disrupting AnkG-KIF5 binding with small interfering RNA or dominant-negative constructs markedly reduced channel levels at axon initial segment (AIS) entire axons,...
Abstract Oxytocin plays an important role in modulating social recognition memory. However, the direct implication of oxytocin neurons paraventricular nucleus hypothalamus (PVH) and their downstream hypothalamic targets regulating short- long-term forms memory has not been fully investigated. In this study, we employed a chemogenetic approach to target activity PVH male rats found that specific silencing neuronal population led impairment We combined viral-mediated fluorescent labeling with...
Abundant evidence indicates that both genetic and environmental factors contribute to the etiology of autism spectrum disorders (ASDs). However, limited knowledge is available concerning these contributing factors. An epidemiology study reported a link between increased incidence living closely major highways, suggesting possible role for pollutants from highway traffic. We investigated whether maternal exposure diesel exhaust particles (DEP) negatively affects fetal development leading...
Repeated exposure to cocaine produces changes in the nervous system that facilitate drug-seeking behaviors. These behaviors have been studied with animal models, such as cocaine-induced locomotor sensitization. Cocaine is hypothesized induce sensitization by neural changes, including an increase density of spines on dendrites neurons nucleus accumbens (NAC). However, how increases dendritic spine NAC has difficult discern because inhibits function multiple targets, transporters for dopamine,...
Abstract Hypothalamic oxytocin (OXT) and arginine-vasopressin (AVP) neurons have been at the center of several physiological behavioral studies. Advances in viral vector biology development transgenic rodent models allowed for targeted gene expression to study functions specific cell populations brain circuits. In this study, we compared efficiency various adeno-associated vectors these demonstrated that none widely used promoters were, on their own, effective driving a down-stream...
Receptor protein tyrosine phosphatase rho ( RPTPρ , gene symbol PTPRT ) is a transmembrane expressed at high levels in the developing hippocampus, olfactory bulb, cortex, and cerebellum. It has an extracellular domain that interacts with other cell adhesion molecules, it two intracellular domains, one of which catalytically active. In recent genome‐wide association study, was identified as potential candidate for autism spectrum disorder ASD susceptibility. Mutation critical aspartate to...
Dopamine signaling in the nucleus accumbens is critical mediating effects of cocaine. There are two splice variants dopamine D2 receptors, D2L and D2S, which believed to have different functional roles. Here, we show, that knocking down selectively using viral-mediated short-hairpin RNA led a slight but significant decrease basal locomotor activity with no change cocaine-induced stimulation locomotion. The knockdown appears produce trend reduced conditioned place preference cocaine...
SUMMARY Oxytocin plays an important role in modulating social recognition memory. However, the direct implication of oxytocin neurons paraventricular nucleus hypothalamus (PVH) and their downstream hypothalamic targets regulating short- long-term forms memory has not been fully investigated. In this study, we employed a chemogenetic approach to target activity PVH male rats found that specific silencing neuronal population led impairment We combined viral mediated fluorescent labeling with...
Social behaviors are crucial for human connection and belonging, often impacted in conditions like Autism Spectrum Disorder (ASD). The mesoaccumbens pathway (VTA NAc) plays a pivotal role social behavior is implicated ASD. However, the impact of ASD-related mutations on reward processing remains insufficiently explored. This study focuses Shank3 mutation, associated with rare genetic condition linked to ASD, examining its influence during behavior, using Shank3-deficient rat model. Our...
The nicotinic α5 receptor subunit, encoded by CHRNA5, harbors multiple functional single nucleotide polymorphisms (SNPs) that affect mRNA expression and alter the protein. These are most notably associated with drug-taking behaviors cognition. We previously identified common SNPs in a distant regulatory element (DRE) increase CHRNA5 human prefrontal cortex (PFC) confer risk for nicotine dependence. Genome-wide epigenetic studies PFC adipose tissue find strong effects of DRE on CpG...
Abstract Glioblastoma (GBM), a deadly brain cancer, infiltrates the and can be synaptically innervated by neurons. Synaptic inputs onto GBM cells identified so far are largely short-range glutamatergic in nature. The extent of integration into brain-wide neuronal circuitry is therefore not well understood. We report application transsynaptic viral tracing approaches to study connectome GBM. applied rabies virus-mediated retrograde herpes simplex virus (HSV)-mediated anterograde characterize...
SUMMARYOxytocin plays an important role in modulating social recognition memory. However, the direct implication of oxytocin neurons paraventricular nucleus hypothalamus (PVH) and their downstream hypothalamic targets regulating short- long-term forms memory is not fully understood. In this study, we employed a chemogenetic approach to specifically target activity PVH rats found that silencing these impaired both long short-term We combined viral mediated fluorescent labeling with...