A5024969393- Nuclear Structure and Function
- RNA Research and Splicing
- RNA regulation and disease
- Cell Image Analysis Techniques
- Peroxisome Proliferator-Activated Receptors
- Olfactory and Sensory Function Studies
- Photochromic and Fluorescence Chemistry
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Genomics and Chromatin Dynamics
- Digital Holography and Microscopy
- Biochemical Analysis and Sensing Techniques
- Advanced Fluorescence Microscopy Techniques
- DNA and Nucleic Acid Chemistry
- Radioactive element chemistry and processing
- Hepatitis C virus research
- RNA and protein synthesis mechanisms
- thermodynamics and calorimetric analyses
Columbia University
2023-2025
Rockefeller University
2016-2020
Abstract Olfactory receptor (OR) choice provides an extreme example of allelic competition for transcriptional dominance, where every olfactory neuron stably transcribes one approximately 2,000 or more OR alleles 1,2 . gene is mediated by a multichromosomal enhancer hub that activates transcription at single 3,4 , followed OR-translation-dependent feedback stabilizes this 5,6 Here, using single-cell genomics, we show formation many competing hubs with variable composition, only which retains...
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Ultra long-range genomic contacts, which emerge as prominent components of genome architecture, constitute a biochemical paradox. This is because regulatory DNA elements make selective and stable contacts with sequences located megabases away, instead interacting proximal occupied by the same exact transcription factors (TF). exemplified in olfactory sensory neurons (OSNs), where only fraction Lhx2/Ebf1/Ldb1-bound sites interact each other, converging into highly multi-chromosomal enhancer...
While the static structure of nuclear pore complex (NPC) continues to be refined with cryo-EM and x-ray crystallography, in vivo conformational changes NPC remain under-explored. We developed sensors that report on orientation components by rigidly conjugating mEGFP different proteins. Our studies show select domains nucleoporins (Nups) within inner ring (Nup54, Nup58, Nup62) when transport through is perturbed no Nups elsewhere NPC. results suggest are flexible undergo upon engaging cargo.
Apolipoprotein E ( ApoE ), a component of very‐low‐density and high‐density lipoproteins, participates in many aspects lipid transport the bloodstream. Underscoring its important functions, isoforms have been associated with metabolic circulatory disease. is also incorporated into hepatitis C virus HCV ) particles, promotes their production infectivity. Live cell imaging analysis behavior during secretion from producing cells thus has potential to reveal details regarding lipoprotein...
The phenylalanine-glycine–repeat nucleoporins (FG-Nups), which occupy the lumen of nuclear pore complex (NPC), are critical for transport between nucleus and cytosol. Although NPCs differ in composition across species, they largely conserved organization function. Transport through is on millisecond timescale. Here, to explore dynamics this timescale, we use coarse-grained computational simulations. These simulations generate predictions that can be experimentally tested distinguish proposed...
Abstract While the static structure of nuclear pore complex (NPC) continues to be refined with cryo-EM and x-ray crystallography, in vivo conformational dynamics NPC remain under-explored. We developed sensors that report on orientation components by rigidly conjugating mEGFP different proteins. Our studies show changes select domains Nups within inner ring (Nup54, Nup58, Nup62) when transport through is perturbed no elsewhere NPC. results suggest are flexible undergo upon engaging cargo.