A5007569728- COVID-19 Clinical Research Studies
- Psychedelics and Drug Studies
- Long-Term Effects of COVID-19
- Pluripotent Stem Cells Research
- Neurotransmitter Receptor Influence on Behavior
- Neurogenesis and neuroplasticity mechanisms
- Pharmacological Receptor Mechanisms and Effects
- Cellular transport and secretion
- SARS-CoV-2 and COVID-19 Research
- Receptor Mechanisms and Signaling
- 3D Printing in Biomedical Research
- Biochemical Analysis and Sensing Techniques
- Advanced Proteomics Techniques and Applications
- Retinal Development and Disorders
- Tryptophan and brain disorders
- Rabies epidemiology and control
- Down syndrome and intellectual disability research
- Hepatitis C virus research
- Microtubule and mitosis dynamics
- Hippo pathway signaling and YAP/TAZ
- Axon Guidance and Neuronal Signaling
- Bacillus and Francisella bacterial research
- Neuroscience and Neuropharmacology Research
- Venomous Animal Envenomation and Studies
- Cannabis and Cannabinoid Research
D’Or Institute for Research and Education
2017-2024
Universidade Federal do Rio de Janeiro
2014-2021
Max Planck Institute of Molecular Cell Biology and Genetics
2008-2019
Instituto Nacional do Câncer
2003
Abstract Zika virus (ZIKV) has been extensively studied since it was linked to congenital malformations, and recent research revealed that astrocytes are targets of ZIKV. However, the consequences ZIKV infection, especially this cell type, remain largely unknown, particularly considering integrative studies aiming understand crosstalk among key cellular mechanisms fates involved in neurotoxicity virus. Here, infection iPSC-derived presented. Our results show ROS imbalance, mitochondrial...
Abstract Background Current approaches of drug repurposing against COVID-19 have not proven overwhelmingly successful and the SARS-CoV-2 pandemic continues to cause major global mortality. nsp12, its RNA polymerase, shares homology in nucleotide uptake channel with HCV orthologue enzyme NS5B. Besides, NS5A has pleiotropic activities, such as binding, that are shared various proteins. Thus, anti-HCV NS5B inhibitors, like sofosbuvir daclatasvir, respectively, could be endowed anti-SARS-CoV-2...
SH-SY5Y neuroblastoma cells are susceptible to differentiation using retinoic acid (RA) and brain-derived neurotrophic factor (BDNF), providing a model of neuronal differentiation. We compared proteome before after RA/BDNF treatment iTRAQ phosphopeptide enrichment strategies. identified 5587 proteins, 366 them with differential abundance. Differentiated expressed proteins related development, and, undifferentiated involved in cell proliferation. Interactive network covered focal adhesion,...
Organoid cultivation in suspension culture requires agitation at low shear stress to allow for nutrient diffusion, which preserves tissue structure. Multiplex systems organoid have been proposed, but whether they meet similar parameters as the regularly used spinner flask and its correlation with successful generation of brain organoids has not determined.Here we computational fluid dynamics (CFD) simulate two multiplex conditions: steering plates on an orbital shaker use a previously...
Abstract Current approaches of drugs repurposing against 2019 coronavirus disease (COVID-19) have not proven overwhelmingly successful and the severe acute respiratory syndrome 2 (SARS-CoV-2) pandemic continues to cause major global mortality. Daclatasvir (DCV) sofosbuvir (SFV) are clinically approved hepatitis C virus (HCV), with satisfactory safety profile. DCV SFV target HCV enzymes NS5A NS5B, respectively. is endowed pleotropic activities, which overlap several proteins from SARS-CoV-2....
Abstract Long-distance axonal trafficking plays a critical role in neuronal function and transport defects have been linked to neurodegenerative disorders. Various lines of evidence suggest that the small GTPase Rab5 signaling via early endosomal transport. Here, we characterized motility endosomes primary cultures mouse hippocampal pyramidal cells by live-cell imaging showed they exhibit bi-directional long-range axons, with strong bias toward retrograde Characterization key effectors...
Coronavirus disease 2019 (COVID-19) was initially described as a viral infection of the respiratory tract. It is now known, however, that several other organs are affected, including brain. Neurological manifestations such stroke, encephalitis, and psychiatric conditions have been reported in COVID-19 patients, but neurotropic potential virus still debated. Herein, we sought to investigate SARS-CoV-2 human neural cells. We demonstrated tissue non-permissive, it can elicit inflammatory...
<i>Dyrk1a</i> triplication in Down9s syndrome and its overexpression Alzheimer9s disease suggest a role for increased DYRK1A activity the abnormal metabolism of APP. Transport defects are early phenotypes progression disease, which lead to APP processing impairments. However, whether regulates intracellular transport delivery human neurons remains unknown. From proteomic dataset cerebral organoids treated with harmine, inhibitor, we found expression changes protein clusters associated...
Abstract Psychedelic agonists of serotonin receptors induce neural plasticity and synaptogenesis, but their potential to enhance learning remains uncharted. Here we show that a single dose d-LSD, potent serotonergic agonist, increased novel object preference in young adult rats several days after treatment. d-LSD alone did not increase old animals, could rescue it levels when followed by 6-day exposure enriched environment (EE). Mass spectrometry-based proteomics human brain organoids...
Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which can infect several organs, especially impacting capacity. Among the extrapulmonary manifestations of COVID-19 myocardial injury, associated with a high risk mortality. Myocardial directly or indirectly SARS-CoV-2 infection, be triggered inflammatory processes that lead to damage heart tissue. Since one hallmarks “cytokine storm”, strategies control inflammation infection have...
SARS-CoV-2 infects cardiac cells and causes heart dysfunction. Conditions such as myocarditis arrhythmia have been reported in COVID-19 patients. The Sigma-1 receptor (S1R) is a ubiquitously expressed chaperone that plays central role cardiomyocyte function. S1R has proposed therapeutic target because it may affect replication; however, the impact of inhibition human cardiomyocytes remains to be described. In this study, we investigated consequences iPSC-derived (hiPSC-CM). infection...
Abstract Coronavirus disease 2019 (COVID-19) was initially described as a viral infection of the respiratory tract. It is now known, however, that several other organs are affected, including brain. Neurological manifestations such stroke, encephalitis, and psychiatric conditions have been reported in COVID-19 patients, but neurotropic potential virus still debated. Herein, we sought to investigate SARS-CoV-2 human neural cells. We demonstrated tissue non-permissive, it can elicit...
ABSTRACT Heart dysfunction, represented by conditions such as myocarditis and arrhythmia, has been reported in COVID-19 patients. Therapeutic strategies focused on the cardiovascular system, however, remain scarce. The Sigma-1 receptor (S1R) recently proposed a therapeutic target because its inhibition reduces SARS-CoV-2 replication. To investigate role of S1R infection heart, we used human cardiomyocytes derived from induced pluripotent stem cells (hiPSC-CM) an experimental model. Here show...
Abstract Entamoeba histolytica causes amoebiasis which is a major health concern in developing countries. E. pathogenicity has been implicated to large repertoire of small GTPases switch between the inactive GDP bound state and active GTP with help g uanine nucleotide e xchange f actors (GEFs) G TPase ctivating p rotein (GAPs). Rho family are well known modulate actin cytoskeletal dynamics plays role pathogenicity. Here, we report an atypical amoebic RhoGEF, its preferred substrate EhRho6,...
ABSTRACT Proteomic analysis of human cerebral organoids may reveal how psychedelics regulate biological processes, shedding light on drug-induced changes in the brain. This study elucidates proteomic alterations induced by lysergic acid diethylamide (LSD) organoids. By employing high-resolution mass spectrometry-based proteomics, we quantitatively analyzed differential abundance proteins exposed to LSD. Our findings indicate proteostasis, energy metabolism, and neuroplasticity-related...
Proteomic analysis of human cerebral organoids may reveal how psychedelics regulate biological processes, shedding light on drug-induced changes in the brain. This study elucidates proteomic alterations induced by lysergic acid diethylamide (LSD) organoids. By employing high-resolution mass spectrometry-based proteomics, we quantitatively analyzed differential abundance proteins exposed to LSD. Our findings indicate proteostasis, energy metabolism, and neuroplasticity-related pathways....