A5060534323- Animal Virus Infections Studies
- Plant Virus Research Studies
- Immune cells in cancer
- Pluripotent Stem Cells Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer Genomics and Diagnostics
- Immunotherapy and Immune Responses
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Chronic Myeloid Leukemia Treatments
- Sarcoma Diagnosis and Treatment
- 3D Printing in Biomedical Research
- Anesthesia and Neurotoxicity Research
- Genetic factors in colorectal cancer
- Biomedical Ethics and Regulation
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Acute Myeloid Leukemia Research
- Viral Infections and Immunology Research
- Neonatal and Maternal Infections
- Neurogenesis and neuroplasticity mechanisms
- Bat Biology and Ecology Studies
- CRISPR and Genetic Engineering
- SARS-CoV-2 and COVID-19 Research
- Hepatitis Viruses Studies and Epidemiology
- Vagus Nerve Stimulation Research
Instituto Estadual do Cérebro Paulo Niemeyer
2020-2024
D’Or Institute for Research and Education
2016-2022
Universidade Federal do Rio de Janeiro
2015
Memorial Sloan Kettering Cancer Center
2010-2013
Ludwig Cancer Research
2010-2013
Fundação Oswaldo Cruz
2003-2012
Hospital Universitário Clementino Fraga Filho
2012
Johns Hopkins University
2011
University of Milan
1995
Istituto Ortopedico Gaetano Pini
1995
Abstract Zika virus (ZIKV) has been extensively studied since it was linked to congenital malformations, and recent research revealed that astrocytes are targets of ZIKV. However, the consequences ZIKV infection, especially this cell type, remain largely unknown, particularly considering integrative studies aiming understand crosstalk among key cellular mechanisms fates involved in neurotoxicity virus. Here, infection iPSC-derived presented. Our results show ROS imbalance, mitochondrial...
Multiply primed rolling-circle amplification is a novel technology that uses bacteriophage phi29 DNA polymerase to amplify circular molecules, without the need for prior knowledge of their sequences. In an attempt detect Torque teno virus (TTV), was used extracted from eight human and four pig serum samples. All samples gave high molecular weight (>30 kb) products. By restriction endonuclease digestion, these products generated fragments whose sizes were consistent with those TTV (3.8 swine...
Liquid biopsy is a non-invasive method used to detect cancer and monitor treatment responses by analyzing blood or other bodily fluids for biomarkers. Meningiomas are the most common primary central nervous system tumors, biomarkers play crucial role in their diagnosis, prognosis, monitoring. The World Health Organization (WHO) classifies meningiomas based on tumor grades molecular alterations genes such as NF2, AKT1, TRAF7, SMO, PIK3CA, KLF4, SMARCE1, BAP1, H3K27me3, TERT promoter,...
Abstract Astrogliosis comprises a variety of changes in astrocytes that occur context‐specific manner, triggered by temporally diverse signaling events vary with the nature and severity brain insults. However, most mechanisms underlying astrogliosis were described using animals, which fail to reproduce some aspects human astroglial signaling. Here, we report an vitro model study human‐induced pluripotent stem cells (iPSC)‐derived replicate intertwined reactive vivo. We analyzed time course...
Urine samples were collected from three patients (2 males, 1 female) with clinically diagnosed Attention Deficit Hyperactivity Disorder (ADHD) according to DSM-5 criteria using semi-structured interviews (K-SADS adapted for adults) by a trained psychiatrist. epithelial cell lines established and expanded subsequent reprogramming procedure. Induced pluripotent stem cells (iPSCs) derived integration-free CytoTune®-iPS 2.0 Sendai Reprogramming Kit, which includes virus particles of the four...
Abstract Human isolates of the highly prevalent TT virus (TTV) have been classified into five major genomic groups (1–5). The geographical distribution throughout world is not well known. Five different PCR assays were developed in an attempt to amplify specifically TTV DNAs each group. Serum samples collected from 72 Brazilian adults (24 voluntary blood donors, 24 hepatitis B (HBV) carriers, and human immunodeficiency type 1 (HIV‐1)‐infected patients) tested. DNA at least one group was...
Background Cancer/testis (CT) genes are expressed only in the germ line and certain tumors most frequently located on X-chromosome (the CT-X genes). Amongst best studied those encoding several MAGE protein families. The function of proteins is not well understood, but have been shown to potentially influence tumorigenic phenotype. Methodology/Principal Findings We undertook a mutational analysis coding regions four genes, MAGEA1, MAGEA4, MAGEC1, MAGEC2 ubiquitously MAGEE1 human melanoma...
Flavonoids are polyphenolic compounds that ubiquitous in plants and have biological effects on cancer cells other cell types. In particular, apigenin (API) has been shown to bind estrogen receptors, which affect the development, maturation, function, plasticity of nervous system. The aim this study was investigate 4′,5,7-trihydroxyflavone upon neural differentiation human pluripotent stem cells. Treatment both embryonic induced with API increased number nestin (NES+) progenitor compared...
Although patterns of somatic alterations have been reported for tumor genomes, little is known on how they compare with present in non-tumor genomes. A comparison the two would be crucial to better characterize genetic driving tumorigenesis. We sequenced genomes a lymphoblastoid (HCC1954BL) and breast (HCC1954) cell line derived from same patient compared both. The genome presents comparable number similar spectrum nucleotide substitutions that found genome. However, significant difference...
Understanding the cellular basis of neurological disorders have advanced at a slow pace, especially due to extreme invasiveness brain biopsying and limitations cell lines animal models that been used. Since derivation pluripotent stem cells (PSCs), novel source for regenerative medicine disease modeling has become available, holding great potential neurology field. However, safety therapy accurateness matter intense debate, considering genomic instability, including gain loss chromosomes...
Peripheral blood sample was donated by a 61years old female patient diagnosed with acute myeloid leukemia secondary to primary myelofibrosis harboring the 52-bp deletion in CALR gene (c.1092_1143del, p.L367fs*46) and R693X mutation ASXL1 (c.2077C>T, p.R693X). CD34+ cells were isolated from subjected reprogramming procedure using Sendai virus carrying factors Oct3/4, Sox2, Klf4 c-Myc. iPS colonies generated retained original mutations displayed all features of bona fide cells.
Skin biopsies were collected from three male patients (age 45, 44 and 44) with clinically diagnosed schizophrenia. The according to DSM-5 criteria by a trained psychiatrist. Dermal fibroblast cell lines established expanded for subsequent reprogramming procedures. Induced pluripotent stem (iPS) cells derived using the integration-free CytoTune®-iPS 2.0 Sendai Reprogramming Kit, containing virus particles of four Yamanaka factors Oct3/4, Sox2, Klf4 c-Myc.
PROM1 is the gene encoding prominin-1 or CD133, an important cell surface marker for isolation of both normal and cancer stem cells. transcripts initiate at a range transcription start sites (TSS) associated with distinct tissue expression profiles. Using high resolution Cap Analysis Gene Expression (CAGE) sequencing we characterize TSS utilization across broad developmental tissues. We identify novel proximal promoter (P6) within CD133(+) melanoma lines Additional exon array sampling finds...
SEN virus (SENV) is a circular, single stranded DNA that has been first characterized in the serum of human immunodeficiency type 1 (HIV-1)-infected patient. Eight genotypes SENV (A-H) have identified and further recognized as variants TT (TTV) family Circoviridae. Here we describe genomic characterization isolate (5-A) from South America. Using 'universal' primers, able to amplify most, if not all, TTV/SENV genotypes, segment > 3 kb was amplified by polymerase chain reaction an HIV-1...
Estudos recentes sobre o torque teno vírus (TTV), gênero Anellovirus, permitiram construir a hipótese de que esse pode ser um desencadeante ou tenha algum papel patogênico nas doenças reumáticas autoimunes. OBJETIVOS: Verificar frequência da infecção pelo TTV em pacientes com lúpus eritematoso sistêmico (LES), e sua diversidade gênica, existência correlação entre as manifestações clínicas do LES, evolução clínica perfil sorológico. PACIENTES E MÉTODOS: Foram obtidas 46 amostras soro LES...
Graphene, including graphene quantum dots, its oxide and unoxidized forms (pure graphene) have several properties, like fluorescence, electrical conductivity, theoretical surface area, low toxicity, high biocompatibility. In this study, we evaluated genotoxicity ( in silico analysis using the functional density theory-FDT), cytotoxicity (human glioblastoma cell line), vivo pharmacokinetics, impact on microcirculation internalization assay. It was also radiolabeled with lutetium 177 (177Lu),...
The Cancer/Testis (CT) antigen family of genes are transcriptionally repressed in most human tissues but atypically re-expressed many malignant tumour types. Their restricted expression profile makes CT antigens ideal targets for cancer immunotherapy. As little is known about whether may be regulated by post-translational processing, we investigated the mechanisms governing degradation NY-ESO-1 and MAGE-C1 selected cell lines. Inhibitors proteasome-mediated induced partitioning into a...
Background: Cancer/testis (CT) genes are expressed only in the germ line and certain tumors most frequently located on X-chromosome (the CT-X genes).Amongst best studied those encoding several MAGE protein families.The function of proteins is not well understood, but have been shown to potentially influence tumorigenic phenotype.Methodology/Principal Findings: We undertook a mutational analysis coding regions four genes, MAGEA1, MAGEA4, MAGEC1, MAGEC2 ubiquitously MAGEE1 human melanoma...