A5085877858- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- bioluminescence and chemiluminescence research
- Single-cell and spatial transcriptomics
- Bone fractures and treatments
- Neuroscience and Neuropharmacology Research
- RNA Research and Splicing
- Cell Image Analysis Techniques
- Mesenchymal stem cell research
- Neurogenesis and neuroplasticity mechanisms
- Bioinformatics and Genomic Networks
- Extracellular vesicles in disease
- Renal and related cancers
- 3D Printing in Biomedical Research
- Animal Genetics and Reproduction
- Psychedelics and Drug Studies
- Tryptophan and brain disorders
- Prenatal Screening and Diagnostics
- Brain Tumor Detection and Classification
- Trace Elements in Health
- Microtubule and mitosis dynamics
- Sarcoma Diagnosis and Treatment
- Cannabis and Cannabinoid Research
- Pancreatic function and diabetes
- Cancer Cells and Metastasis
Broad Institute
2020-2024
Harvard University
2020-2023
Instituto Nacional de Traumatologia e Ortopedia
2017-2021
D’Or Institute for Research and Education
2016-2019
Universidade Federal do Rio de Janeiro
2009-2017
National Institute of Traumatology & Orthopaedic Rehabilitation
2017
Hospital Universitário Clementino Fraga Filho
2012
Fundo Brasil
2012
Institute of Biomedical Science
2009
Abstract Dimethyltryptamines are entheogenic serotonin-like molecules present in traditional Amerindian medicine recently associated with cognitive gains, antidepressant effects, and changes brain areas related to attention. Legal restrictions the lack of adequate experimental models have limited understanding how such substances impact human metabolism. Here we used shotgun mass spectrometry explore proteomic differences induced by 5-methoxy-N,N-dimethyltryptamine (5-MeO-DMT) on cerebral...
The limited access to functional human brain tissue has led the development of stem cell-based alternative models. differentiation pluripotent cells into cerebral organoids with self-organized architecture created novel opportunities study early stages formation. Here we applied state-of-the-art label-free shotgun proteomics compare proteome cell-derived fetal brain. We identified 3,073 proteins associated different developmental stages, from neural progenitors neurons, astrocytes, or...
Future clinical applications of human embryonic stem (hES) cells will require high-yield culture protocols. Currently, hES are mainly cultured in static tissue plates, which offer a limited surface and repeated sub-culturing. Here we describe stirred system with commercial dextran-based microcarriers coated denatured collagen to scale-up cell production. Maintenance pluripotency the microcarrier-based was shown by immunocytochemical flow cytometry analyses for pluripotency-associated...
Abstract Astrocytes play a critical role in the development and homeostasis of central nervous system (CNS). Astrocyte dysfunction results several neurological degenerative diseases. However, major challenge to our understanding astrocyte physiology pathology is restriction studies animal models, human post-mortem brain tissues, or samples obtained from invasive surgical procedures. Here, we report protocol generate functional astrocytes cerebral organoids derived pluripotent stem cells. The...
Glioblastoma (GBM) is characterized by heterogeneous malignant cells that are functionally integrated within the neuroglial microenvironment. In this study, we model ecosystem growing GBM into long-term cultured human cortical organoids contain major cell types found in cerebral cortex. Single-cell RNA sequencing analysis suggests that, compared with matched gliomasphere models, more faithfully recapitulate diversity and expression programs of states patient tumors. Additionally, observe...
<p>Supplementary Figure S5 describes extracellular vesicle mediated intercellular transfer in GCOs, related to 5.</p>
<p>Supplementary Figure S3 describes GFP transfer process in GCOs, related to 3.</p>
<p>Text file shows the GFP sequence from pLV-CMV-GFP used to align reads scRNA-seq data.</p>
<p>Supplementary Figure S4 describes widespread transfer of endogenous malignant transcripts in GCOs, related to 4.</p>
<p>Supplementary Figure S2 describes malignant cell states in GCOs, related to 2.</p>
<p>Supplementary Figure S1 describes the establishment and scRNA-seq profiling of GCOs, related to 1.</p>
<div>Abstract<p>Glioblastoma (GBM) is characterized by heterogeneous malignant cells that are functionally integrated within the neuroglial microenvironment. In this study, we model ecosystem growing GBM into long-term cultured human cortical organoids contain major cell types found in cerebral cortex. Single-cell RNA sequencing analysis suggests that, compared with matched gliomasphere models, more faithfully recapitulate diversity and expression programs of states patient...
De novo heterozygous loss-of-function mutations in phosphatase and tensin homolog (PTEN) are strongly associated with autism spectrum disorders; however, it is unclear how this gene affect different cell types during human brain development these effects vary across individuals. Here, we used cortical organoids from donors to identify cell-type specific developmental events that affected by PTEN. We profiled individual single-cell RNA-seq, proteomics spatial transcriptomics revealed...
The existence of loss and gain chromosomes, known as aneuploidy, has been previously described within the central nervous system. During development, at least one-third neural progenitor cells (NPCs) are aneuploid. Notably, aneuploid NPCs may survive functionally integrate into mature circuitry. Given unanswered significance this phenomenon, we tested hypothesis that differentiation induced by all-trans retinoic acid (RA) in pluripotent stem is accompanied increased levels for cortical vivo....
Although human embryonic stem cells (hESC) have been shown to present a stable diploid karyotype 1, many studies reported that depending on culture conditions they become prone acquire chromosomal anomalies such as addition of whole or parts chromosomes. Indeed, during long-term culture, karyotypic alterations are observed when enzymatic chemical dissociation used 2,3,4, while manual dissection colonies for passaging retains 5. Besides, changes in the environment removal feeder also seem...
Synovial fluid holds a population of mesenchymal stem cells (MSC) that could be used for clinical treatment. Our goal was to characterize the inflammatory and metabolomic profile synovial from osteoarthritic patients identify its modulatory effect on cells. collected non-OA OA patients, which centrifuged isolate Cells were cultured 21 days, characterized with specific markers MSC, exposed cocktail induce chondrogenic, osteogenic, adipogenic differentiation. Then, we performed MTT assay...
ABSTRACT Genetic risk for autism spectrum disorder (ASD) has been associated with hundreds of genes spanning a wide range biological functions. The phenotypic alterations in the human brain resulting from mutations ASD remain unclear, and level at which these converge on shared disease pathology is poorly understood. Here, we leveraged reproducible organoid models cerebral cortex to identify cell type-specific developmental abnormalities haploinsufficiency three genes, SUV420H1 ( KMT5B ),...
Understanding the cellular basis of neurological disorders have advanced at a slow pace, especially due to extreme invasiveness brain biopsying and limitations cell lines animal models that been used. Since derivation pluripotent stem cells (PSCs), novel source for regenerative medicine disease modeling has become available, holding great potential neurology field. However, safety therapy accurateness matter intense debate, considering genomic instability, including gain loss chromosomes...
Systematic studies of micronutrients during brain formation are hindered by restrictions to animal models and adult post-mortem tissues. Recently, advances in stem cell biology have enabled recapitulation the early stages human telencephalon development vitro. In present work, we analyzed cerebral organoids derived from pluripotent cells synchrotron radiation X-ray fluorescence order measure biologically valuable incorporated distributed into exogenously developing brain. Our findings...