A5029791890- Advanced Proteomics Techniques and Applications
- Cellular transport and secretion
- Epigenetics and DNA Methylation
- Neuroinflammation and Neurodegeneration Mechanisms
- Long-Term Effects of COVID-19
- Neurogenesis and neuroplasticity mechanisms
- 14-3-3 protein interactions
- Metabolomics and Mass Spectrometry Studies
- Tryptophan and brain disorders
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Mass Spectrometry Techniques and Applications
- RNA Research and Splicing
- COVID-19 Clinical Research Studies
- Diet and metabolism studies
- Glycosylation and Glycoproteins Research
- Pluripotent Stem Cells Research
- COVID-19 and Mental Health
- Wnt/β-catenin signaling in development and cancer
- Neuropeptides and Animal Physiology
- Sirtuins and Resveratrol in Medicine
- Neuroscience and Neural Engineering
- Neurological disorders and treatments
- Bipolar Disorder and Treatment
- Bioinformatics and Genomic Networks
Universidade Estadual de Campinas (UNICAMP)
2015-2024
University of California, San Diego
2023
National Council for Scientific and Technological Development
2017
Although increasing evidence confirms neuropsychiatric manifestations associated mainly with severe COVID-19 infection, long-term dysfunction (recently characterized as part of "long COVID-19" syndrome) has been frequently observed after mild infection. We show the spectrum cerebral impact acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ranging from alterations in mildly infected individuals (orbitofrontal cortical atrophy, neurocognitive impairment, excessive fatigue and anxiety...
Phosphorylation of α-synuclein at the serine-129 site (α-syn Ser129P) is an established pathologic hallmark synucleinopathies and a therapeutic target. In physiologic states, only fraction α-syn phosphorylated this site, most studies have focused on roles post-translational modification. We found that unlike wild-type (WT) α-syn, which widely expressed throughout brain, overall pattern Ser129P restricted, suggesting intrinsic regulation. Surprisingly, preventing blocked activity-dependent...
The limited access to functional human brain tissue has led the development of stem cell-based alternative models. differentiation pluripotent cells into cerebral organoids with self-organized architecture created novel opportunities study early stages formation. Here we applied state-of-the-art label-free shotgun proteomics compare proteome cell-derived fetal brain. We identified 3,073 proteins associated different developmental stages, from neural progenitors neurons, astrocytes, or...
Abstract Astrocytes play a critical role in the development and homeostasis of central nervous system (CNS). Astrocyte dysfunction results several neurological degenerative diseases. However, major challenge to our understanding astrocyte physiology pathology is restriction studies animal models, human post-mortem brain tissues, or samples obtained from invasive surgical procedures. Here, we report protocol generate functional astrocytes cerebral organoids derived pluripotent stem cells. The...
Abstract Although increasing evidence confirms neuropsychiatric manifestations associated mainly with severe COVID-19 infection, the long-term dysfunction has been frequently observed after mild infection. Here we show spectrum of cerebral impact SARS-CoV-2 infection ranging from alterations in mildly infected individuals (orbitofrontal cortical atrophy, neurocognitive impairment, excessive fatigue and anxiety symptoms) to acute damage confirmed brain tissue samples extracted orbitofrontal...
Abstract COVID-19 patients may exhibit neuropsychiatric and neurological symptoms. We found that anxiety cognitive impairment are manifested by 28-56% of SARS-CoV-2-infected individuals with mild respiratory symptoms associated altered cerebral cortical thickness. Using an independent cohort, we histopathological signs brain damage in 25% who died COVID-19. All the affected tissues exhibited foci SARS-CoV-2 infection replication, particularly astrocytes. Infection neural stem cell-derived...
Abstract Background Schizophrenia is a complex and severe neuropsychiatric disorder, with wide range of debilitating symptoms. Several aspects its multifactorial complexity are still unknown, some accepted to be an early developmental deficiency more specifically neurodevelopmental origin. Understanding the timepoints disturbances during neural cell differentiation processes could lead insight into development disorder. In this context, human brain organoids cells differentiated from...
Schizophrenia (SCZ) is a serious neuropsychiatric disorder that manifests through several symptoms from early adulthood. Numerous studies over the last decades have led to significant advances in increasing our understanding of factors involved SCZ. For example, mass spectrometry-based proteomic analysis has provided important insights by uncovering protein dysfunctions inherent Here, we present comprehensive nuclear proteome postmortem brain tissues corpus callosum (CC) and anterior...
Abstract The COVID-19 pandemic was initiated by the rapid spread of a SARS-CoV-2 strain. Though mainly classified as respiratory disease, infects multiple tissues throughout human body, leading to wide range symptoms in patients. To better understand how affects proteome from cells with different ontologies, this work generated an infectome atlas 9 cell models, including brain, blood, digestive system, and adipocyte tissue. Our data shows that infection trigger dysregulations on proteins...
Two protein post-translational modifications, lysine succinylation and malonylation, are implicated in regulation, glycolysis, energy metabolism. The precursors of these succinyl-CoA malonyl-CoA, key players central metabolic processes. Both modification profiles have been proven to be responsive stimuli, such as hypoxia. As mitochondrial dysfunction dysregulation schizophrenia other psychiatric illnesses, the potential reveal yet another layer regulation can furthermore represent targets...
Exosomes are cell-derived nano vesicles that play multiple roles as mediate cellular communication, elimination of undesirable components and transport molecules such RNAs proteins.Misfolded proteins associated to the development pathologies may be also transported by exosomes.This study aims perform a quantitative proteome analysis exosomes collected from cell cultures derived patients with schizophrenia mentally healthy controls, in order find differentially expressed possibly disease.
Schizophrenia (SCZ) is a chronic and mental disorder characterized by the presence of positive, negative cognitive symptons.Although molecular mechanisms involved in SCZ still unknow, there dysfunction on energy metabolism cellular signaling.To clarify potential linking between these pathways, we enriched three compartments carried out bottom up proteomics annalysis to find proteins related pathophysiology disease.
A number of basic and translational studies have clearly indicated the vital role glia in brain function pathophysiological mechanisms neuropsychiatric disorders, including schizophrenia. The difficulty on studying molecular basis glial cells vivo, led to development animal models, which are considered gold standard this type understanding. However, inherent difficulties establishing these models for psychiatric disorders simplicity vitro especially given recent advances stem cell-based...