A5032850472- Sperm and Testicular Function
- Mosquito-borne diseases and control
- Reproductive Biology and Fertility
- Neurogenesis and neuroplasticity mechanisms
- Tryptophan and brain disorders
- Mitochondrial Function and Pathology
- Neuroscience and Neuropharmacology Research
- Psychedelics and Drug Studies
- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- Advanced Proteomics Techniques and Applications
- Axon Guidance and Neuronal Signaling
- Biochemical Analysis and Sensing Techniques
- Berberine and alkaloids research
- Neurotransmitter Receptor Influence on Behavior
- Nerve injury and regeneration
- Venomous Animal Envenomation and Studies
- Fatty Acid Research and Health
- Chemical synthesis and alkaloids
- Cancer, Hypoxia, and Metabolism
- Education Pedagogy and Practices
- 14-3-3 protein interactions
- Neuroinflammation and Neurodegeneration Mechanisms
- Cellular Mechanics and Interactions
- Neurobiology and Insect Physiology Research
Universidade Federal de São Paulo
2021-2025
Universidade Federal do Rio de Janeiro
2020-2025
Universidade Estadual de Campinas (UNICAMP)
2015-2024
D’Or Institute for Research and Education
2015-2024
Pontifical Catholic University of Puerto Rico
2024
Pontifícia Universidade Católica do Paraná
2024
Universidade Iguaçu
2024
Universidade Norte do Paraná
2024
Universidade Federal do Estado do Rio de Janeiro
2019
University of Freiburg
2012-2017
Zika virus tested in human brain organoids The pernicious and resilient Aedes mosquito is rapidly spreading (ZIKV) through the Americas. ZIKV infection mostly causes mild disease, but some patients, nervous system involvement indicated. A particular worry an observed correlation between of mothers first trimester pregnancy microcephaly newborns. Garcez et al. effects compared with dengue on neural stem cells grown as organoids. targeted cells, reduced their size viability vitro, caused...
Abstract Zika virus (ZIKV) has been associated with microcephaly and other brain abnormalities; however, the molecular consequences of ZIKV to human development are still not fully understood. Here we describe alterations in neurospheres derived from induced pluripotent stem (iPS) cells infected strain that is circulating Brazil. Combining proteomics mRNA transcriptional profiling, over 500 proteins genes Brazilian infection were found be differentially expressed. These provide an...
Abstract Dimethyltryptamines are entheogenic serotonin-like molecules present in traditional Amerindian medicine recently associated with cognitive gains, antidepressant effects, and changes brain areas related to attention. Legal restrictions the lack of adequate experimental models have limited understanding how such substances impact human metabolism. Here we used shotgun mass spectrometry explore proteomic differences induced by 5-methoxy-N,N-dimethyltryptamine (5-MeO-DMT) on cerebral...
Background Birch bark has a long lasting history as traditional medicinal remedy to accelerate wound healing. Recently, the efficacy of birch preparations also been proven clinically. As active principle pentacyclic triterpenes are generally accepted. Here, we report comprehensive study on underlying molecular mechanisms healing properties well-defined preparation named TE (triterpene extract) well isolated single in human primary keratinocytes and porcine ex-vivo models....
The limited access to functional human brain tissue has led the development of stem cell-based alternative models. differentiation pluripotent cells into cerebral organoids with self-organized architecture created novel opportunities study early stages formation. Here we applied state-of-the-art label-free shotgun proteomics compare proteome cell-derived fetal brain. We identified 3,073 proteins associated different developmental stages, from neural progenitors neurons, astrocytes, or...
Abstract Astrocytes play a critical role in the development and homeostasis of central nervous system (CNS). Astrocyte dysfunction results several neurological degenerative diseases. However, major challenge to our understanding astrocyte physiology pathology is restriction studies animal models, human post-mortem brain tissues, or samples obtained from invasive surgical procedures. Here, we report protocol generate functional astrocytes cerebral organoids derived pluripotent stem cells. The...
Harmine is the β -carboline alkaloid with highest concentration in psychotropic plant decoction Ayahuasca. In rodents, classical antidepressants reverse symptoms of depression by stimulating neuronal proliferation. It has been shown that Ayahuasca presents antidepressant effects patients depressive disorder. present study, we investigated harmine cell cultures containing human neural progenitor cells (hNPCs, 97% nestin-positive) derived from pluripotent stem cells. After 4 days treatment,...
Since the emergence of Zika virus (ZIKV), reports microcephaly have increased dramatically in Brazil; however, causality between widespread epidemic and malformations fetal brains has not been confirmed. Here, we examine effects ZIKV infection human neural stem cells growing as neurospheres cerebral organoids. Using immunocytochemistry electron microscopy, show that targets brain cells, reducing their viability growth These results suggest abrogates neurogenesis during development.
Consumption of alcoholic beverages during pregnancy is directly related to the establishment fetal alcohol spectrum disorders (FASD), which includes craniofacial changes, body growth restriction, and neurodevelopment impairments. Proper functioning central nervous system (CNS) depends on blood-brain barrier (BBB) development, formed by interactions vascular endothelial cells, pericytes, astrocytes, basal lamina. Gestational exposure ethanol has been demonstrated impair CNS development;...
Contents The purpose of this study was to validate a technique for simultaneous evaluation the plasma, acrosomal and mitochondrial membranes in boar spermatozoa, using an association fluorescent probes: Propidium iodide (PI), fluorescein isothiocyanate‐conjugated Pisum sativum agglutinin (FITC‐PSA) JC‐1. Three ejaculates from each four different boars, all showing motility ≥80% abnormal morphology ≤10%, were diluted TALP medium split into two aliquots. One aliquots flash frozen liquid...
Abstract Motivation: Cell migration is a complex process that controlled through the time-sequential feedback regulation of protein signalling and gene regulation. Based on prior knowledge own experimental data, we developed large-scale dynamic network describing onset maintenance hepatocyte growth factor-induced primary human keratinocytes. We applied Boolean logic to capture qualitative behaviour as well short-and long-term dynamics involved in this process, comprising signalling,...
Olfaction plays a critical role in several aspects of the human life. Odorants are detected by hundreds odorant receptors (ORs) which belong to superfamily G protein-coupled receptors. These expressed olfactory sensory neurons nose. The information provided activation different combinations ORs nose is transmitted brain, leading perception and emotional behavioral responses. There ~400 intact ORs, date only small percentage these (~10%) have known agonists. determination specificity will...
In this experiment, it was defined a protocol of fluorescent probes combination: propidium iodide (PI), fluorescein isothiocyanate-conjugated Pisum sativum agglutinin (FITC-PSA), and JC-1. For purpose, four ejaculates from three different rams (n=12), all showing motility >80% abnormal morphology <10%, were diluted in TALP medium split into two aliquots. One the aliquots flash frozen thawed continuous cycles, to induce damage cellular membranes disturb mitochondrial function. Three...
The objective was to verify the relationship between equine semen cryopreservation and changes related increased lipid peroxidation. Also, addition of autologous or homologous seminal plasma from a stallion with good freezing response post-thawed sperm tested determine whether it would confer protection. Frozen-thawed were evaluated allocated into three groups: without addition, supplemented either plasma. All groups at 0, 60 120 min after incubation 37 °C. Cryopreservation did not increase...
Keratinocytes account for 95% of all cells the epidermis, stratified squamous epithelium forming outer layer skin, in which a significant number skin diseases takes root. Immortalized keratinocyte cell lines are often used as research model systems providing standardized, reproducible, and homogenous biological material. Apart from that, primary human keratinocytes frequently medical studies because provides an important route drug administration is readily accessible biopsies. However,...