A5010355097- Circadian rhythm and melatonin
- Molecular Biology Techniques and Applications
- Adipose Tissue and Metabolism
- Genomics and Phylogenetic Studies
- Cell Adhesion Molecules Research
- Light effects on plants
- Biomarkers in Disease Mechanisms
- Genomics, phytochemicals, and oxidative stress
- Microbial Natural Products and Biosynthesis
- Mesenchymal stem cell research
- 14-3-3 protein interactions
- Bone Metabolism and Diseases
- Single-cell and spatial transcriptomics
- Bone health and treatments
- Photoreceptor and optogenetics research
- Estrogen and related hormone effects
- Angiogenesis and VEGF in Cancer
- Pregnancy and preeclampsia studies
- Frailty in Older Adults
- Fibroblast Growth Factor Research
- Fatty Acid Research and Health
- RNA Research and Splicing
- Connective Tissue Growth Factor Research
- Fungal and yeast genetics research
- Protein Kinase Regulation and GTPase Signaling
Biomechanics Institute of Valencia
2024
Institute for Biomedical Engineering
2024
ETH Zurich
2022-2024
MRC Weatherall Institute of Molecular Medicine
2022-2024
University of Oxford
2019-2024
MRC Human Immunology Unit
2022-2023
Heidelberg University
2019-2023
Heidelberg University
2019
University of Freiburg
2012-2017
Max Planck Institute for Molecular Biomedicine
2017
Blood and lymphatic vessels form a versatile transport network provide inductive signals to regulate tissue-specific functions. in bone osteogenesis hematopoiesis, but current dogma suggests that lacks vessels. Here, by combining high-resolution light-sheet imaging cell-specific mouse genetics, we demonstrate presence of human bones. We find expand during genotoxic stress. VEGF-C/VEGFR-3 signaling stress-induced IL6 drive lymphangiogenesis During lymphangiogenesis, secretion CXCL12 from...
3D tissue imaging reveals maladaptations of blood vessels in organ aging, fibrosis, and arthritis.
Bone provides supportive microenvironments for hematopoietic stem cells (HSCs) and mesenchymal (MSCs) is a frequent site of metastasis. While incidences bone metastases increase with age, the properties marrow microenvironment that regulate dormancy reactivation disseminated tumor (DTCs) remain poorly understood. Here, we elucidate age-associated changes in secretome trigger proliferation HSCs, MSCs, DTCs aging microenvironment. Remarkably, bone-specific mechanism involving expansion...
Endovenous laser ablation (EVLA) is a very effective minimal invasive procedure to treat the patients of symptomatic lower limb varicosities with low complication rate, recurrence, early mobilisation and less duration in-hospital stay. Aims objectives: To evaluates outcomes EVLA treatment in terms symptom relief, cosmetic improvement, rates, recovery time, long-term efficacy. Materials methods: This was single centre retrospective study conducted 540 having diagnosis unilateral or bilateral...
Abstract Motivation: Cell migration is a complex process that controlled through the time-sequential feedback regulation of protein signalling and gene regulation. Based on prior knowledge own experimental data, we developed large-scale dynamic network describing onset maintenance hepatocyte growth factor-induced primary human keratinocytes. We applied Boolean logic to capture qualitative behaviour as well short-and long-term dynamics involved in this process, comprising signalling,...
The nerve growth factor NGF has been shown to cause cell fate decisions towards either differentiation or proliferation depending on the relative activity of downstream pERK, pAKT pJNK signaling. However, how these protein signals are translated into and fed back from transcriptional complete cellular over a time span hours days is still an open question. Comparing time-resolved transcriptome response NGF- EGF-stimulated PC12 cells 24 in combination with phenotype data we inferred dynamic...
Here, we present a protocol for using spatial transcriptomics in bone and multi-tissue musculoskeletal formalin-fixed paraffin-embedded (FFPE) samples from mice. We describe steps tissue harvesting, sample preparation, paraffin embedding, FFPE selection. detail procedures sectioning placement on slides prior to imaging, decrosslinking, library final analyses of the sequencing data. The complete takes ca. 18 days mouse femora with adjacent muscle; this time, >50% is required mineralized...
Abstract The mammalian skeletal system shows sex differences in structure, functions, aging and disease incidences. role of blood vessels physiological, regenerative pathological bone functions indicates the requisite to understanding their specificity. In this study, we found that estrogen regulates vessel physiology during pregnancy menopause through receptor alpha (ERα) G-protein-coupled receptor-1 (GPER1) but not ERβ-dependent signaling mice. Estrogen lipid use endothelial cells (BECs)...
Ageing is an inherent and intricate biological process that takes place in living organisms as time progresses. It involves the decline of multiple physiological functions, leading to body structure overall performance modifications. The ageing differs among individuals influenced by various factors, including lifestyle, environment genetic makeup. Metabolic changes reduced locomotor activity are common hallmarks ageing. Our study focuses on exploring these phenomena prematurely PolgA...
Frailty, a geriatric syndrome, is assessed using the frailty phenotype (FP) and index (FI). While these approaches have been applied to aging mice, their effectiveness in prematurely mouse models such as PolgA D257A/D257A (PolgA) has not completely explored. We demonstrated that became evident mice around 40 weeks, validated through body weight loss, reduced walking speed, decreased physical activity, weaker grip strength. Moreover, we also identified sex differences with females exhibiting...
Experiments have recently shown that p53 expression can display oscillations in response to certain stress signals. In this work, mathematical modelling and bifurcation analysis are combined investigate under which conditions the oscillation of could propagate its direct downstream transcription targets. The authors' suggests will only proteins with medium-fast mRNA protein turnover rates. authors retrieved data concerning half-life for a number p53-promoted genes found that, according their...
Eukaryotic circadian clocks are based on self-sustaining, cell-autonomous oscillatory feedback loops that can synchronize with the environment via recurrent stimuli (zeitgebers) such as light. The components of biological and their network interactions becoming increasingly known, calling for a quantitative understanding role clock function. However, development data-driven mathematical models has remained limited by lack sufficiently accurate data. Here we present comprehensive model...
A correct genome annotation is fundamental for research in the field of molecular and structural biology. The reference Chaetomium thermophilum has been reported previously, but it essentially limited to open reading frames (ORFs) protein coding genes contains only a few noncoding transcripts. In this study, we identified annotated full-length transcripts C. by deep RNA sequencing. We 7044 4567 genes. Astonishingly, 23% are alternatively spliced. 679 novel as well 2878 corrected organization...
Abstract The skeletal structure and bone marrow endothelium collectively form a critical functional unit essential for development, health, aging. At the core of osteogenesis formation lies dynamic process angiogenesis. In this study, we reveal potent new endogenous anabolic NCAM1-14.3.3.ζδ-derived-Peptide interaction, which stimulates angiogenesis during homeostasis, aging, age-related diseases. Employing high-resolution imaging inducible cell-specific mouse genetics, our results elucidate...
Abstract Ageing is an inherent and intricate biological process that takes place in living organisms as time progresses. It involves the decline of multiple physiological functions, leading to body structure overall performance modifications. The ageing differs among individuals influenced by various factors, including lifestyle, environment, genetic makeup. Metabolic changes reduced locomotor activity are common hallmarks ageing. Our study focuses on exploring these phenomena prematurely...
Abstract Transient activation via the B cell receptor (BCR) primes naïve cells to respond more efficiently CD40 signals, resulting in increased division over a 48hr time course. At this point approximately 40% of primed had undergone at least one division, as compared 15% activated by anti-CD40 antibody alone. Cells that divided once continued through several additional cycles absence exogenous stimuli, whereas not after first required renewed anti-Ig signals induce proliferation. These...
In Brief: Current dogma is that lymphatic vessels are absent in bone and marrow. Using advanced 3D-imaging mouse genetics, these authors show the presence of lymph bone. Moreover, they genotoxic stress causes expansion lymphangiogenesis bone, which turn promotes hematopoietic regeneration.
A correct genome annotation is fundamental for research in the field of molecular and structural biology. The reference Chaetomium thermophilum has been reported previously, but it limited to open reading frames (ORFs) genes contains only a few noncoding transcripts. In this study, we identified annotated by deep RNA sequencing full-length transcripts C.thermophilum. We 7044 coding large number (n=4567). Astonishingly, 23% are alternatively spliced. 679 novel corrected organization more than...