A5030022776- Hematopoietic Stem Cell Transplantation
- Mesenchymal stem cell research
- Cancer, Hypoxia, and Metabolism
- Bone and Joint Diseases
- Immune cells in cancer
- Bone health and treatments
- Acute Myeloid Leukemia Research
- Hematological disorders and diagnostics
- Neonatal Respiratory Health Research
- Zebrafish Biomedical Research Applications
- Cancer Cells and Metastasis
- Bone Metabolism and Diseases
- Cytokine Signaling Pathways and Interactions
- Osteoarthritis Treatment and Mechanisms
- Estrogen and related hormone effects
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Developmental Biology and Gene Regulation
- Fibroblast Growth Factor Research
- Cell Image Analysis Techniques
- Venous Thromboembolism Diagnosis and Management
- Pluripotent Stem Cells Research
- Lymphatic System and Diseases
- Biomarkers in Disease Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- 3D Printing in Biomedical Research
Nanyang Technological University
2025
MRC London Institute of Medical Sciences
2017-2023
Imperial College London
2016-2023
NIHR Imperial Biomedical Research Centre
2020
Max Planck Institute for Molecular Biomedicine
2014-2017
University of Münster
2014-2016
Weizmann Institute of Science
2016
MRC Clinical Trials Unit at UCL
2016
National Centre for Cell Science
2007-2010
Abstract While blood vessels play important roles in bone homeostasis and repair, fundamental aspects of vascular function the skeletal system remain poorly understood. Here we show that long vasculature generates a peculiar flow pattern, which is for proper angiogenesis. Intravital imaging reveals vessel growth murine involves extension anastomotic fusion endothelial buds. Impaired leads to defective angiogenesis osteogenesis, downregulation Notch signalling cells. In aged mice, activity...
Bone marrow vascular niches sustain hematopoietic stem cells (HSCs) and are drastically remodeled in leukemia to support pathological functions. Acute myeloid (AML) produce angiogenic factors, which likely contribute this remodeling, but anti-angiogenic therapies do not improve AML patient outcomes. Using intravital microscopy, we found that progression leads differential remodeling of vasculature central endosteal bone regions. Endosteal pro-inflammatory cytokines gradually degrade...
Blood and lymphatic vessels form a versatile transport network provide inductive signals to regulate tissue-specific functions. in bone osteogenesis hematopoiesis, but current dogma suggests that lacks vessels. Here, by combining high-resolution light-sheet imaging cell-specific mouse genetics, we demonstrate presence of human bones. We find expand during genotoxic stress. VEGF-C/VEGFR-3 signaling stress-induced IL6 drive lymphangiogenesis During lymphangiogenesis, secretion CXCL12 from...
Abstract A population of monocytes, known as Ly6C lo patrol blood vessels by crawling along the vascular endothelium. Here we show that endothelial cells control their origin through Notch signalling. Using combinations conditional genetic deletion strategies and cell-fate tracking experiments Notch2 regulates conversion hi monocytes into in vivo vitro, thereby regulating monocyte cell fate under steady-state conditions. This process is controlled ligand delta-like 1 (Dll1) expressed a...
Highlights•Detailed 3D reconstruction of the calvarial bone marrow microvasculature•Blood flow dynamics at cellular resolution in microvessels•Detailed map with hemodynamic parameters arteries and highly branched sinusoids•Cellular HSPC homing to sinusoidsSummaryMeasurements velocities level individual arterial vessels sinusoidal capillaries are crucial for understanding hematopoietic stem progenitor cell vasculature. We have developed two complementary intravital two-photon imaging...
3D tissue imaging reveals maladaptations of blood vessels in organ aging, fibrosis, and arthritis.
Bone provides supportive microenvironments for hematopoietic stem cells (HSCs) and mesenchymal (MSCs) is a frequent site of metastasis. While incidences bone metastases increase with age, the properties marrow microenvironment that regulate dormancy reactivation disseminated tumor (DTCs) remain poorly understood. Here, we elucidate age-associated changes in secretome trigger proliferation HSCs, MSCs, DTCs aging microenvironment. Remarkably, bone-specific mechanism involving expansion...
Resource20 November 2020Open Access Transparent process Decreased blood vessel density and endothelial cell subset dynamics during ageing of the endocrine system Junyu Chen Tissue Tumor Microenvironments Group, The Kennedy Institute Rheumatology, University Oxford, UK Department Prosthodontics, State Key Laboratory Oral Diseases, West China Hospital Stomatology, Sichuan University, Chengdu, Search for more papers by this author Luciana Lippo Rossella Labella Sin Lih Tan Brian D Marsden...
Abstract The tumor microenvironment (TME) orchestrates cancer progression by fostering a complex interplay between cells and the surrounding cellular acellular elements. Through dynamic interactions with cells, vascular stromal not only promote growth but also enhance metastatic potential restrict therapeutic responses. Vascular play critical role in regulating epithelial-mesenchymal transition (EMT) sustaining resistance pathways, making them compelling targets for innovative therapies....
Notch dictates multiple developmental events, including stem cell maintenance and differentiation, through intercellular communication. However, its temporal influence during early development and, of particular interest, regulation binary fate decision at different stages neurogenesis are among the least explored. Here, using an embryonic (ESC) model, we have deciphered ligand preference ESC commitment to germ layers determined stage-specific effect neural differentiation. ESCs remain...
Abstract The mammalian skeletal system shows sex differences in structure, functions, aging and disease incidences. role of blood vessels physiological, regenerative pathological bone functions indicates the requisite to understanding their specificity. In this study, we found that estrogen regulates vessel physiology during pregnancy menopause through receptor alpha (ERα) G-protein-coupled receptor-1 (GPER1) but not ERβ-dependent signaling mice. Estrogen lipid use endothelial cells (BECs)...
The neural induction has remained a debatable issue pertaining to whether it is mere default process or involves precise instructive cues. We have chosen the embryonic stem (ES) cell model address this issue. In devised monoculture strategy, cell-cell interaction availed through optimum plating density could define niche for attainment of efficient in vitro neurogenesis from ES cells. medium was found ideal generating number progenitors and also yielded about 80% mature neurons serum free...