A5015003756- Angiogenesis and VEGF in Cancer
- Pluripotent Stem Cells Research
- Congenital heart defects research
- COVID-19 Clinical Research Studies
- Atherosclerosis and Cardiovascular Diseases
- Tissue Engineering and Regenerative Medicine
- Long-Term Effects of COVID-19
- 3D Printing in Biomedical Research
- Liver Disease Diagnosis and Treatment
- Biomedical Ethics and Regulation
- Cardiovascular Disease and Adiposity
- Mesenchymal stem cell research
- S100 Proteins and Annexins
- Systemic Lupus Erythematosus Research
- Cell Adhesion Molecules Research
- Cardiovascular Function and Risk Factors
- Acute Ischemic Stroke Management
- Kawasaki Disease and Coronary Complications
- Analytical Chemistry and Sensors
- Cancer, Hypoxia, and Metabolism
- Retinal Diseases and Treatments
- Retinal Imaging and Analysis
- Nuclear Receptors and Signaling
- Cellular Mechanics and Interactions
- Cardiac Fibrosis and Remodeling
Nanyang Technological University
2016-2025
Institute of Molecular and Cell Biology
2016-2025
Agency for Science, Technology and Research
2016-2025
University of Wisconsin–Madison
2023
National Centre for Infectious Diseases
2022
University of California, Berkeley
2019
John Wiley & Sons (United States)
2019
Hudson Institute
2019
Wellcome/MRC Cambridge Stem Cell Institute
2018
University of British Columbia
2017
Numerous reports of vascular events after an initial recovery from COVID-19 form our impetus to investigate the impact on health recovered patients. We found elevated levels circulating endothelial cells (CECs), a biomarker injury, in convalescents compared healthy controls. In particular, those with pre-existing conditions (e.g., hypertension, diabetes) had more pronounced activation hallmarks than non-COVID-19 patients matched cardiovascular risk. Several proinflammatory and activated T...
Chronic diabetic wounds are a significant global healthcare challenge. Current strategies, such as biomaterials, cell therapies, and medical devices, however, only target few pathological features have limited efficacy. A powerful platform technology combining magneto-responsive hydrogel, cells, wireless magneto-induced dynamic mechanical stimulation (MDMS) is developed to accelerate wound healing. The hydrogel encapsulates U.S. Food Drug Administration (FDA)-approved fibroblasts...
Abstract Sustained hypercoagulability and endotheliopathy are present in convalescent COVID‐19 patients for up to 4 months from recovery. The hemostatic, endothelial, inflammatory profiles of 39 recovered were evaluated 16 after recovery COVID‐19. These values compared with a control group healthy volunteers ( n = 124). (71.8% males, median age 43 years) reviewed at mean 12.7 ± 3.6 following One patient without cardiovascular risk factors had post acute ischaemic limb. Elevated D‐dimer...
Bone marrow-derived mesenchymal stem cell (MSC) is one of the most actively studied types due to its regenerative potential and immunomodulatory properties. Conventional expansion methods using 2D tissue culture plates 2.5D microcarriers in bioreactors can generate large numbers, but they compromise potency lack mechanical preconditioning prepare MSC for physiological loading expected
Identifying genetic markers for heterogeneous complex diseases such as heart failure is challenging and requires prohibitively large cohort sizes in genome-wide association studies to meet the stringent threshold of statistical significance. On other hand, chromatin quantitative trait loci, elucidated by direct epigenetic profiling specific human tissues, may contribute toward prioritizing subthreshold variants disease association.
Different vascular beds show differing susceptibility to the development of atherosclerosis, but molecular mechanisms underlying these differences are incompletely understood. This study aims identify factors that contribute phenotypic heterogeneity distinct regions adult vasculature.High-throughput mRNA profiling in mice reveals higher expression homeobox paralogous genes 6 10 (Hox6-10) athero-resistant thoracic aorta (TA) than athero-susceptible aortic arch (AA). Higher gene also occurs...
SARS-CoV-2, the causative agent of COVID-19, is a threat to public health. Evidence suggests increased neutrophil activation and endothelial glycocalyx (EG) damage are independently associated with severe COVID-19. Here, we hypothesised that an level blood myeloperoxidase (MPO) soluble EG breakdown, inhibiting MPO activity may reduce damage.Analysing subset acute convalescent COVID-19 plasma, 10 from 15 non-severe cases, 9 pre-COVID-19 controls, determined levels, proteins (syndecan-1...
Abstract The tumor microenvironment (TME) orchestrates cancer progression by fostering a complex interplay between cells and the surrounding cellular acellular elements. Through dynamic interactions with cells, vascular stromal not only promote growth but also enhance metastatic potential restrict therapeutic responses. Vascular play critical role in regulating epithelial-mesenchymal transition (EMT) sustaining resistance pathways, making them compelling targets for innovative therapies....
Abstract Human pluripotent stem cell‐derived endothelial cells (hPSC‐ECs) present an attractive alternative to primary EC sources for vascular grafting. However, there is a need mature them towards either arterial or venous subtype. A vital environmental factor involved in the arteriovenous specification of ECs during early embryonic development fluid shear stress; therefore, have been attempts employ adult stress conditions hPSC‐ECs. hPSC‐ECs are naïve stress, and their responses still not...
Innovative scaffold fabrication, angiogenesis promotion, and dynamic tissue culture techniques have been utilized to improve delivery of media into the core large constructs in engineering. We developed here an intra-tissue perfusion (ITP) system, which incorporates array seven micron-sized needles as a conduit, mass transfer thick liver tissues slices (>>300 microm transport limit). The ITP system improves uniformity distribution throughout tissue, resulting improved cell viability over...
Abstract Vascular smooth muscle cells (SMCs) from distinct anatomic locations derive different embryonic origins. Here we investigated the respective potential of origin-specific SMCs derived human stem (hESCs) to support endothelial network formation in vitro. three embryological origins were an mStrawberry-expressing hESC line and cocultured with green fluorescent protein-expressing umbilical vein (HUVECs) investigate effects SMC subtypes on formation. Quantitative analysis demonstrated...
COVID-19 management guidelines have largely attributed critically ill patients who develop acute respiratory distress syndrome, to a systemic overproduction of pro-inflammatory cytokines. Cardiovascular dysfunction may also represent primary phenomenon, with increasing data suggesting that severe reflects confluence vascular dysfunction, thrombosis and dysregulated inflammation. Here, we first consolidate the information on localized microvascular inflammation disordered cytokine release,...
Background Myocardin is thought to have a key role in smooth muscle cell (SMC) development by acting on CArG-dependent genes. However, it unclear whether myocardin-induced SMC maturation and increases agonist-induced calcium signalling are also associated with the expression of non-CArG-dependent SMC-specific Moreover, unknown myocardin promotes from human embryonic stem cells. Methodology/Principal Findings The effects adenoviral-mediated overexpression ESC-derived embryoid bodies were...