A5061190740- Angiogenesis and VEGF in Cancer
- Axon Guidance and Neuronal Signaling
- Zebrafish Biomedical Research Applications
- Hippo pathway signaling and YAP/TAZ
- Congenital heart defects research
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Wnt/β-catenin signaling in development and cancer
- Chromatin Remodeling and Cancer
- Bone Metabolism and Diseases
- Developmental Biology and Gene Regulation
- DNA Repair Mechanisms
- Genetic Associations and Epidemiology
- Genetic diversity and population structure
- Cellular Mechanics and Interactions
- Pregnancy and preeclampsia studies
- TGF-β signaling in diseases
- Forensic and Genetic Research
- Cancer Cells and Metastasis
- Parasitic Infections and Diagnostics
- Fibroblast Growth Factor Research
- Circular RNAs in diseases
- Parasitic infections in humans and animals
- Apelin-related biomedical research
Max Planck Institute for Molecular Biomedicine
2010-2025
University of Münster
2010-2022
Max Planck Institute for Biophysical Chemistry
2013
Max Planck Society
2005-2009
Victor Babeș University of Medicine and Pharmacy Timișoara
2003-2005
The functional diversity of the arterial and venous endothelia is regulated through a complex system signalling pathways downstream transcription factors. Here we report that factor Sox17, which known as regulator endoderm hemopoietic differentiation, selectively expressed in arteries, not veins, mouse embryo postnatal retina adult. Endothelial cell-specific inactivation Sox17 accompanied by lack differentiation vascular remodelling results death utero. In retina, abrogation expression...
Abstract Tissue vascularization entails the formation of a blood vessel plexus, which remodels into arteries and veins. Here we show, by using time-lapse imaging zebrafish fin regeneration genetic lineage tracing endothelial cells in mouse retina, that vein-derived tip contribute to emerging arteries. Our movies uncover arterial-fated change migration direction migrate backwards within expanding vascular plexus. This behaviour critically depends on chemokine receptor cxcr4a function. We show...
Abstract VEGFR-2/Notch signalling regulates angiogenesis in part by driving the remodelling of endothelial cell junctions and inducing migration. Here, we show that VEGF-induced polarized elongation increases perimeter decreases relative VE-cadherin concentration at junctions, triggering formation actin-driven junction-associated intermittent lamellipodia (JAIL) under control WASP/WAVE/ARP2/3 complex. JAIL allow new adhesion sites are critical for migration monolayer integrity. Whereas...
Abstract Cell segregation allows the compartmentalization of cells with similar fates during morphogenesis, which can be enhanced by cell fate plasticity in response to local molecular and biomechanical cues. Endothelial tip growing retina, lead vessel sprouts, give rise arterial endothelial thereby mediate growth. Here, we have combined type-specific inducible mouse genetics, flow experiments vitro, single-cell RNA sequencing biochemistry show that balance between ephrin-B2 its receptor...
Abstract Notch and Angiopoietin-1 (Ang1)/Tie2 pathways are crucial for vascular maturation stability. Here we identify the transcription factor ERG as a key regulator of endothelial signalling. We show that controls balance between ligands by driving Delta-like ligand 4 (Dll4) while repressing Jagged1 (Jag1) expression. In vivo , this regulation occurs selectively in maturing plexus mouse developing retina, where Ang1/Tie2 signalling is active. find mediates Ang1-dependent required...
The microvasculature of the central nervous system includes blood-brain barrier (BBB), which regulates permeability to nutrients and restricts passage toxic agents inflammatory cells. Canonical Wnt/β-catenin signaling is responsible for early phases brain vascularization BBB differentiation. However, this signal declines after birth, other pathways able maintain integrity at postnatal stage are still unknown.Sox17 (SRY [sex-determining region Y]-box 17) constitutes a major downstream target...
For a skin wound to successfully heal, the cut epidermal-edge cells have migrate forward at interface between scab and healthy granulation tissue. Much is known about how lead-edge migrate, but very little mechanisms that enable active participation by further back. Here we show ephrin-B1 its receptor EphB2 are both upregulated in vivo, just for duration of repair, first 70 or so rows epidermal cells, this signal leads downregulation molecular components adherens tight (but not desmosomal)...
Declining bone mass is associated with aging and osteoporosis, a disease characterized by progressive weakening of the skeleton increased fracture incidence. Growth lifelong homeostasis rely on interactions between different cell types including vascular cells mesenchymal stromal (MSCs). As these involve Notch signaling, we have explored whether treatment secreted ligand proteins can enhance osteogenesis in adult mice. We show that bone-targeting, high affinity version Delta-like 4, termed...
The homeostasis of heart and other organs relies on the appropriate provision nutrients functional specialization local vasculature. Here, we have used mouse genetics, imaging cell biology approaches to investigate how in adult is controlled by endothelial EphB4 its ligand ephrin-B2, which are known regulators vascular morphogenesis arteriovenous differentiation during development. We show that inducible cell-specific inactivation Ephb4 mice compatible with survival, but leads rupturing...
<title>Abstract</title> Blood vessels provide a versatile and adaptable transport system, but recent work has established that endothelial cells, which form the innermost lining of vascular network, are also source molecular signals controlling behavior other cell types in surrounding tissue. Pericytes another essential component vessel wall, comparably little is known about their signaling interactions with populations during organ growth patterning. Here, we have used tissue-specific...
Abstract Background The re-replication inhibitor Geminin binds to several transcription factors including homeodomain proteins, and members of the polycomb SWI/SNF complexes. Results Here we describe TATA-binding protein-like factor-interacting protein (TIPT) isoform 2, as a strong binding partner Geminin. TIPT2 is widely expressed in mouse embryonic adult tissues, residing both cyto- nucleoplasma, enriched nucleolus. Like Geminin, also interacts with factors, general factor TBP (TATA box...