A5081629684- Genetics, Aging, and Longevity in Model Organisms
- Circadian rhythm and melatonin
- Axon Guidance and Neuronal Signaling
- Spaceflight effects on biology
- Cellular Mechanics and Interactions
- Spectroscopy Techniques in Biomedical and Chemical Research
- Force Microscopy Techniques and Applications
- Nanoparticles: synthesis and applications
- Neuroscience and Neuropharmacology Research
- Plant Reproductive Biology
- Gut microbiota and health
- Elasticity and Material Modeling
- Genomics, phytochemicals, and oxidative stress
- History of Education in Spain
- Plant-Microbe Interactions and Immunity
- Hippo pathway signaling and YAP/TAZ
- Biochemical effects in animals
- Image Processing Techniques and Applications
- Fungal and yeast genetics research
- Advanced X-ray and CT Imaging
- Insect and Arachnid Ecology and Behavior
- Angiogenesis and VEGF in Cancer
- Neurobiology and Insect Physiology Research
- Parasite Biology and Host Interactions
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
University of Freiburg
2023-2024
University College London
2016-2023
Imperial College London
2020-2021
MRC London Institute of Medical Sciences
2020
Hammersmith Hospital
2020
MRC Laboratory for Molecular Cell Biology
2018
Goethe University Frankfurt
2008-2014
Max Planck Institute of Neurobiology
2007
Max Planck Society
2007
Abstract Solid tumours are exposed to microenvironmental factors such as hypoxia that normally inhibit cell growth. However, tumour cells capable of counteracting these signals through mechanisms largely unknown. Here we show the prolyl hydroxylase PHD3 restrains growth in response cues control EGFR. silencing human gliomas or genetic deletion a murine high-grade astrocytoma model markedly promotes and ability continue growing under unfavourable conditions. The growth-suppressive function is...
Apical extracellular matrices (aECMs) form a physical barrier to the environment. In Caenorhabditis elegans, epidermal aECM, cuticle, is composed mainly of different types collagen, associated in circumferential ridges separated by furrows. Here, we show that mutants lacking furrows, normal intimate connection between epidermis and cuticle lost, specifically at lateral epidermis, where, contrast dorsal ventral there are no hemidesmosomes. At ultrastructural level, profound alteration...
Abstract Genetic and environmental factors are key drivers regulating organismal lifespan but how these impact healthspan is less well understood. Techniques capturing biomechanical properties of tissues on a nano-scale level providing new insights into disease mechanisms. Here, we apply Atomic Force Microscopy (AFM) to quantitatively measure the change in associated with ageing Caenorhabditis elegans addition high-resolution topographical images cuticle senescence. We show that distinct...
Little is known about the possibility of reversing age-related biological changes when they have already occurred. To explore this, we characterized effects reducing insulin/IGF-1 signaling (IIS) during old age. Reduction IIS throughout life slows decline in diverse species, most strikingly nematode Caenorhabditis elegans. Here show that even at advanced ages, auxin-induced degradation DAF-2 single tissues, including neurons and intestine, still able to markedly increase C. elegans lifespan....
Understanding how animals detect and respond to pathogen threats is central dissecting mechanisms of host immunity. The oomycetes represent a diverse eukaryotic group infecting various hosts from nematodes humans. We have previously shown that Caenorhabditis elegans mounts defense response consisting the induction chitinase-like (chil) genes in epidermis combat infection by its natural oomycete Myzocytiopsis humicola. provide here evidence C. can sense detecting an innocuous extract derived...
Behavioural phenotyping of model organisms is widely used to investigate fundamental aspects organism biology, from the functioning nervous system effects genetic mutations, as well for screening new drug compounds. However, our capacity observe and quantify full range complexity behavioural responses limited by inability conventional microscopy techniques capture volumetric image information at sufficient speed. In this article we describe how combining light field with computational depth...
Oomycetes are a group of eukaryotic organisms that includes many important pathogens animals and plants. Within this group, the Haptoglossa genus is characterised by presence specialised gun cells carrying harpoon-like infection apparatus. While several have been morphologically described, there currently no host systems developed to study process or responses in lab. In study, we report species potent natural Caenorhabditis nematodes. Using electron microscopy, characterise C. elegans...
Abstract The nematode Caenorhabditis elegans is commonly used as a model organism in studies of the host immune response. worm encodes twelve peroxidase-cyclooxygenase superfamily members, making it an attractive which to study functions heme peroxidases. In previous work, loss one these peroxidases, SKPO-1 (ShkT-containing peroxidase), rendered C. more sensitive human, Gram-positive pathogen Enterococcus faecalis. was localized hypodermis animals where also affected cuticle development...
The small GTPase RhoA plays a crucial role in the regulation of neuronal signalling to generate behaviour. In developing nervous system is known regulate actin cytoskeleton, however effectors RhoA-signalling adult neurons remain largely unidentified. We have previously shown that activation ortholog (RHO-1) C. elegans cholinergic motor triggers hyperactivity these and loopy locomotion with exaggerated body bends. This achieved part through increased diacylglycerol (DAG) levels recruitment...
The function of a specific tissue and its biomechanics are interdependent, with pathologies or ageing often being intertwined structural decline. Caenorhabditis elegans , model organism widely used in pharmacological research, has been established as biomarker for healthy ageing. However, the properties constituent tissues, their contribution to overall mechanical characteristics organism, remain relatively unknown. In this study we investigated C. cuticle, muscle tissue, pseudocoelom using...
Intraoperative frozen section analysis can be used to improve the accuracy of tumour margin estimation during cancer resection surgery through rapid processing and pathological assessment excised tissue. Its applicability is limited in some cases due additional risks associated with prolonged surgery, largely from time-consuming staining procedure. Our work uses a measurable property bulk tissue bypass process: as cells proliferate, they influence surrounding extra-cellular matrix, resulting...
ABSTRACT The function of a specific tissue and its biomechanics are interdepended, with pathologies or ageing often being intertwined structural decline. Caenorhabditis elegans ( C. ), model organism widely used in pharmacological research, has been established as biomarker for healthy ageing, though the mechanics individual tissues have remained elusive. In this study we investigated cuticle, muscle tissue, pseudocoelom using combination indentation experiments silico modelling. Nematode...
Prostate cancer is now the most commonly diagnosed in men western countries. Due to difficulty for early detection, there are an estimated 10000 deaths a year UK from prostate alone; whereby only curative option interventional treatment that aims excise all diseased cells while preserving neurovascular bundle. To date, several studies have shown mechanical properties of and tissues i.e. adhesion, stiffness, roughness viscoelasticity significantly different benign regions tissue healthy....