A5053777031- Inorganic Chemistry and Materials
- Solid-state spectroscopy and crystallography
- Microwave and Dielectric Measurement Techniques
- Magnetism in coordination complexes
- Metallurgy and Material Science
- Material Properties and Applications
- Magnetic properties of thin films
- Gestational Trophoblastic Disease Studies
- Optical Coatings and Gratings
- Crystal Structures and Properties
- Liquid Crystal Research Advancements
- Phase-change materials and chalcogenides
- Metallurgical and Alloy Processes
- Lymphoma Diagnosis and Treatment
- Thermodynamic and Structural Properties of Metals and Alloys
- Lanthanide and Transition Metal Complexes
- Coastal and Marine Management
- Polyoxometalates: Synthesis and Applications
- Semiconductor Lasers and Optical Devices
- Chalcogenide Semiconductor Thin Films
- Rare-earth and actinide compounds
- Superconducting Materials and Applications
- Soil erosion and sediment transport
- Physics of Superconductivity and Magnetism
- Peatlands and Wetlands Ecology
MRC London Institute of Medical Sciences
2017-2025
Imperial College London
2016-2025
American University
2025
Victoria University of Wellington
2020-2024
NIHR Imperial Biomedical Research Centre
2017-2023
Faculty (United Kingdom)
2018-2021
Hammersmith Hospital
2021
Medical Research Council
2011-2020
University of Chicago
2020
Transnational Press London
2018
Blood clots and thrombi consist primarily of a mesh branched fibers made the protein fibrin. We propose molecular basis for marked extensibility negative compressibility fibrin gels based on structural mechanical properties at network, fiber, levels. The force required to stretch clot initially rises linearly is accompanied by dramatic decrease in volume peak compressibility. These macroscopic transitions are fiber alignment bundling after forced unfolding. Constitutive models developed...
Avian A/H5N1 influenza viruses pose a pandemic threat. As few as five amino acid substitutions, or four with reassortment, might be sufficient for mammal-to-mammal transmission through respiratory droplets. From surveillance data, we found that two of these substitutions are common in viruses, and thus, some require only three additional to become transmissible via droplets between mammals. We used mathematical model within-host virus evolution study factors could increase decrease the...
Tissue cells lack the ability to see or hear but have evolved mechanisms feel into their surroundings and sense a collective stiffness. A cell can even effective stiffness of rigid objects that are not in direct cellular contact—like proverbial princess who feels pea placed beneath soft mattresses. How deeply matrix be measured by assessing responses on controlled series thin elastic gels affixed substrate. Gel elasticity E is readily varied with polymer concentrations now-standard...
Visible phenotypes based on locomotion and posture have played a critical role in understanding the molecular basis of behavior development Caenorhabditis elegans other model organisms. However, it is not known whether these human-defined features capture most important aspects for phenotypic comparison or they are sufficient to discover new behaviors. Here we show that four basic shapes, eigenworms, previously described wild-type worms, also mutant this representation can be used build...
We study the role of microtubule movement in bidirectional organelle transport Drosophila S2 cells and show that EGFP-tagged peroxisomes serve as sensitive probes motor induced, noisy cytoskeletal motions. Multiple move unison over large time windows correlations with tip positions, indicating rapid fluctuations longitudinal direction. report first high-resolution measurement performed by tracing such pairs co-moving peroxisomes. The resulting picture shows motor-dependent oscillations...
Journal Article Hyaluronic acid matrices show matrix stiffness in 2D and 3D dictates cytoskeletal order myosin-II phosphorylation within stem cells Get access Florian Rehfeldt, Rehfeldt Biophysical Eng'g. Lab, University of Pennsylvania, Philadelphia 19104, PA, USA. Tel: +1 215 898 48093rd Institute Physics-Biophysics, Georg-August-University, Friedrich-Hund-Platz 1, 37077 Göttingen, Germany. Fax: +49 551 397720; 3913831 E-mail: rehfeldt@physik3.gwdg.de Search for other works by this author...
Loss of proteostasis is a fundamental process driving aging. Proteostasis affected by the accuracy translation, yet physiological consequence having fewer protein synthesis errors during multi-cellular organismal aging poorly understood. Our phylogenetic analysis RPS23, key in ribosomal decoding center, uncovered lysine residue almost universally conserved across all domains life, which replaced an arginine small number hyperthermophilic archaea. When introduced into eukaryotic RPS23...
Various biological activities have been attributed to actin-capping proteins based on their in vitro effects actin filaments. However, there is little direct evidence for vivo activities. In this paper, we show that Cap Z(36/32), a barbed end, protein isolated from muscle (Casella, J. F., D. Maack, and S. Lin, 1986, Biol. Chem., 261:10915-10921) localized the ends of filaments by electron microscopy Z-line chicken skeletal indirect immunofluorescence microscopy. Since associate with at ends,...
Crystallization processes are in general sensitive to detailed conditions, but our present understanding of underlying mechanisms is insufficient. A crystallizable chain within a diblock copolymer assembly expected couple curvature crystallization and thereby impact rigidity as well preferred morphology, the effects on dispersed phases have remained unclear. The hydrophobic polymer polycaprolactone (PCL) semi-crystalline bulk (T(m) = 60°C) shown here generate flexible worm micelles or rigid...
The active regulation of cellular forces during cell adhesion plays an important role in the determination size, shape and internal structure. While on flat, homogeneous isotropic substrates some cells spread isotropically, others anisotropically assume elongated structures. In addition, their native environment as well vitro experiments, spreading asymmetry can be modulated by local distribution adhesive molecules topography environment. We present a simple elastic model, experiments stem...
Behaviour is a sensitive and integrative readout of nervous system function therefore an attractive measure for assessing the effects mutation or drug treatment on animals. Video data provide rich but high-dimensional representation behaviour, so first step analysis often some form tracking feature extraction to reduce dimensionality while maintaining relevant information. Modern machine-learning methods are powerful notoriously difficult interpret, handcrafted features interpretable do not...
Locomotion is driven by shape changes coordinated the nervous system through time; thus, enumerating an animal's complete repertoire of transitions would provide a basis for comprehensive understanding locomotor behaviour. Here we introduce discrete representation behaviour in nematode C. elegans. At each point time, worm's posture approximated its closest matching template from set 90 postures and locomotion represented as sequences postures. The frequency distribution postural heavy-tailed...
Tracking small laboratory animals such as flies, fish, and worms is used for phenotyping in neuroscience, genetics, disease modelling, drug discovery. An imaging system with sufficient throughput spatiotemporal resolution would be capable of a large number animals, estimating their pose, quantifying detailed behavioural differences at scale where hundreds treatments could tested simultaneously. Here we report an array six 12-megapixel cameras that record all the wells 96-well plate to...
Regularities in animal behaviour offer insights into the underlying organizational and functional principles of nervous systems automated tracking provides opportunity to extract features directly from large-scale video data. Yet how effectively analyse such behavioural data remains an open question. Here, we explore whether a minimum description length principle can be exploited identify meaningful behaviours phenotypes. We apply dictionary compression algorithm sequences nematode worm...
To become and remain functional, individual neuron types must select during development maintain throughout life their distinct terminal identity features, such as expression of specific neurotransmitter receptors, ion channels neuropeptides. Here, we report a molecular mechanism that enables cholinergic motor neurons (MNs) in the C. elegans ventral nerve cord to unique identity. This relies on dual function conserved selector UNC-3 (Collier/Ebf). synergizes with LIN-39 (Scr/Dfd/Hox4-5)...
Abstract Genetic and environmental factors are key drivers regulating organismal lifespan but how these impact healthspan is less well understood. Techniques capturing biomechanical properties of tissues on a nano-scale level providing new insights into disease mechanisms. Here, we apply Atomic Force Microscopy (AFM) to quantitatively measure the change in associated with ageing Caenorhabditis elegans addition high-resolution topographical images cuticle senescence. We show that distinct...