A5055285686- Genetics, Aging, and Longevity in Model Organisms
- Amyotrophic Lateral Sclerosis Research
- Microbial Community Ecology and Physiology
- Neurobiology and Insect Physiology Research
- Mitochondrial Function and Pathology
- Fungal and yeast genetics research
- Gut microbiota and health
- CRISPR and Genetic Engineering
- Phytochemicals and Antioxidant Activities
- Circadian rhythm and melatonin
- Genetics, Bioinformatics, and Biomedical Research
- Natural Antidiabetic Agents Studies
- IoT-based Smart Home Systems
- IoT and GPS-based Vehicle Safety Systems
- Robotics and Automated Systems
- Cardiac Fibrosis and Remodeling
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Nematode management and characterization studies
- Heavy Metals in Plants
- Zebrafish Biomedical Research Applications
- Nuclear Physics and Applications
- Neuroscience and Neuropharmacology Research
- Light effects on plants
- Insects and Parasite Interactions
- Polymer Science and Applications
Imperial College London
2003-2024
MRC London Institute of Medical Sciences
2019-2024
McGill University
2024
NIHR Imperial Biomedical Research Centre
2020-2023
Babraham Institute
2023
North South University
2015-2021
Genomics (United Kingdom)
2018-2020
To become and remain functional, individual neuron types must select during development maintain throughout life their distinct terminal identity features, such as expression of specific neurotransmitter receptors, ion channels neuropeptides. Here, we report a molecular mechanism that enables cholinergic motor neurons (MNs) in the C. elegans ventral nerve cord to unique identity. This relies on dual function conserved selector UNC-3 (Collier/Ebf). synergizes with LIN-39 (Scr/Dfd/Hox4-5)...
A hexanucleotide repeat expansion GGGGCC in the non-coding region of C9orf72 is most common cause inherited amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Toxic dipeptide repeats (DPRs) are synthesized from via repeat-associated non-AUG (RAN) translation. Here, we develop C. elegans models that express, either ubiquitously or exclusively neurons, 75 flanked by intronic sequence. The worms generate DPRs (poly-glycine-alanine [poly-GA], poly-glycine-proline [poly-GP])...
Summary The Rac-GEF P-Rex1 mediates GPCR signalling by activating the small GTPase Rac. We show here that also controls trafficking. inhibits agonist-stimulated internalisation of S1PR1 independently its activity, through PDZ, DEP and IP4P domains. limits agonist-induced trafficking CXCR4, PAR4, GLP1R, but does not control steady-state levels, nor RTKs PDGFR EGFR. blocks phosphorylation required for internalisation. binds Grk2, both in vitro cells, appear to regulate Grk2 activity. propose...
Terminal selectors are transcription factors (TFs) that establish during development and maintain throughout life post-mitotic neuronal identity. We previously showed UNC-3/Ebf, the terminal selector of C. elegans cholinergic motor neurons (MNs), acts indirectly to prevent alternative identities (Feng et al., 2020). Here, we globally identify direct targets UNC-3. Unexpectedly, find suite UNC-3 in MNs is modified across different stages, revealing 'temporal modularity' function. In all...
Amyotrophic lateral sclerosis can be caused by abnormal accumulation of TAR DNA-binding protein 43 (TDP-43) in the cytoplasm neurons. Here, we use a C. elegans model for TDP-43-induced toxicity to identify biological mechanisms that lead disease-related phenotypes. By applying deep behavioral phenotyping and subsequent dissection neuromuscular circuit, show TDP-43 worms have profound defects GABA Moreover, acetylcholine neurons appear functionally silenced. Enhancing functional output...
ABSTRACT Nervous system function critically relies on continuous expression of neuron type-specific terminal identity features, such as neurotransmitter receptors, ion channels and neuropeptides. How individual types select features during development maintain them throughout life is poorly understood. Here, we report an unconventional mechanism that enables cholinergic motor neurons (MNs) in the C. elegans ventral nerve cord to their distinct features. The conserved selector UNC-3...
Rac GTPases are required for neutrophil adhesion and migration, the effector responses that kill pathogens. These Rac-dependent functions impaired when neutrophils lack activators of Rac, Rac-GEFs from Prex, Vav, Dock families. In this study, we demonstrate Tiam1 is also expressed in neutrophils, governing focal complexes, actin cytoskeletal dynamics, polarisation, a manner depending on integrin ligand to which cells adhere. dispensable generation reactive oxygen species but mediates...
Food fibers are recently gaining interest in the scientific community for their beneficial role various diseases such as diabetes, cardiovascular diseases, cancer and neurodegenerative diseases. Rice bran is an important source of food fibers. The objective this study was to determine presence polyphenolic compounds rice Aush Dhan vitro antioxidant activities different extracts ethanol, ethylacetate toluene extracts. Various assays, including DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical...
Abstract The gut microbiota harbors a complex ecosystem of bacteria that govern host health homeostasis. Alterations to the intestinal environment, known as dysbiosis, is associated with several diseases. Targeting microbiome-engineered therapeutics, such probiotics, promising approach restore microbial homeostasis and health. Probiotics can effectively improve although strain-specific mechanisms remain largely unknown. Thus, this study aims identify beneficial action select probiotics...
This protocol is a guide of how to prepare the worms be used in COPAS (wormsorter)
C. elegans is maintained in the laboratory on Nematode Growth Medium (NGM) agar which has been aseptically poured into petri plates. Smaller plates (35 mm diameter) are useful for mating or when using expensive drugs. size (60 general strain maintenance, and larger (90 growing quantities of worms, such as certain mutant screens. The NGM medium can be easily a peristaltic pump. This pump adjusted so that constant amount dispensed each plate. A reduces need refocusing microscope you switch...
Amyotrophic lateral sclerosis (ALS) and frontotemporal dementia are caused by the abnormal accumulation of TAR DNAbinding protein 43 (TDP-43) in cytoplasm neurons. How TDP-43 leads to disease symptoms is not wellcharacterized. Here, we use a C. elegans model for TDP-43-induced toxicity identify biological mechanisms that lead disease-related phenotypes. By applying deep behavioral phenotyping, established phenotypic fingerprint TDP43 worms. This was compared 294 mutants, which genes were...
Abstract Amyotrophic lateral sclerosis (ALS) and frontotemporal dementia are caused by the abnormal accumulation of TAR DNA-binding protein 43 (TDP-43) in cytoplasm neurons. How TDP-43 leads to disease symptoms is not well-characterized. Here, we use a C. elegans model for TDP-43-induced toxicity identify biological mechanisms that lead disease-related phenotypes. By applying deep behavioral phenotyping, established phenotypic fingerprint worms. This was compared 294 mutants, which genes...
Before the OP50 solution is aliquoted into small falcon tubes, it always better to check OD by using a spectrophotometer, for documentation purposes.
If you only need a small number of semi-synchronized worms or if simply to remove yeast bacterial contaminants, this protocol is easier and less time-consuming than the full-scale egg prep. This procedure also called ‘spot bleaching’.
Caenorhabditis elegans, a small (adults are ~1 mm long), free-living soil nematode that feeds on bacteria, is an ideal organism for applying various live microscopy techniques. The transparent, thus it possible to microscopically analyze and record the whole animal throughout its entire life. Its complete cell lineage known, making follow developmental differentiation processes in real time.
C. elegans is maintained in the laboratory on Nematode Growth Medium (NGM) agar which has been aseptically poured into petri plates. Smaller plates (35 mm diameter) are useful for mating or when using expensive drugs. size (60 general strain maintenance, and larger (100 growing quantities of worms, such as certain mutant screens. The NGM medium can be easily a peristaltic pump. This pump adjusted so that constant amount dispensed each plate. A reduces need refocusing microscope you switch...
Aldicarb is a cholinesterase inhibitor which prevents the breakdown of acetylcholine in synapse Reported to paralyze C. elegans Strains interest believed be resistant paralytic effect Strains: OW1601 & OW1603