A5076234756- Pluripotent Stem Cells Research
- Wheat and Barley Genetics and Pathology
- Ruminant Nutrition and Digestive Physiology
- Antimicrobial Peptides and Activities
- CRISPR and Genetic Engineering
- Plant Pathogens and Resistance
- Immune Cell Function and Interaction
- Cell Adhesion Molecules Research
- Turfgrass Adaptation and Management
- Neuroscience and Neuropharmacology Research
- Congenital heart defects research
- Plant Disease Resistance and Genetics
- Receptor Mechanisms and Signaling
- Tendon Structure and Treatment
- Tribology and Lubrication Engineering
- Plant Taxonomy and Phylogenetics
- Teratomas and Epidermoid Cysts
- Plant Reproductive Biology
- Collagen: Extraction and Characterization
- Plant Molecular Biology Research
- Bee Products Chemical Analysis
- Genetic diversity and population structure
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Myofascial pain diagnosis and treatment
- Blood disorders and treatments
Babraham Institute
2020-2025
Medical Research Council
2023-2024
Stem Cell Institute
2024
Wellcome Trust
2000-2024
Altos Labs
2024
Wellcome/MRC Cambridge Stem Cell Institute
2023
Wellcome Sanger Institute
2001
New Frontier
2000
John Innes Centre
1996-1999
Aberystwyth University
1994-1998
Collagen is a structural protein whose internal cross-linking critically determines the properties and functions of connective tissue. Knowing how collagen changes with age key to understanding why mechanical tissues change over lifetime. The current scientific consensus that increases this increase leads tendon stiffening. Here, we show view should be reconsidered. Using MS-based analyses, demonstrated during aging healthy C57BL/6 mice, overall levels in tail decreased age. However, lysine...
ABSTRACT Naïve epiblast cells in the embryo and pluripotent stem vitro undergo developmental progression to a formative state competent for lineage specification. During this transition, transcription factors chromatin are rewired encode new functional features. Here, we examine role of mitogen-activated protein kinase (ERK1/2) signalling transition. We show that primary consequence ERK activation mouse embryonic is elimination Nanog, which precipitates breakdown naïve gene regulatory...
Clonal expansion of antigen-specific B-cells defines effective germinal centre responses and is key for the generation high-affinity antibodies. While positive selection in centres has been associated with anabolic metabolism cell growth, downstream drivers B-cell proliferation are not well understood. Here we report that RNA helicase DDX1 required maturation accrual dark-zone cellularity. Upon interaction T-follicular helper cells, DDX1-deficient upregulate c-MYC but do clonally expand. We...
Embryonic regulators are often re-expressed in cancers, however the functional and molecular significance of this is not always understood. The epigenetic priming factors Developmental Pluripotency Associated 2 4 (DPPA2/4) have crucial roles early development implicated cancer pathogenesis. We reveal non-small cell lung (NSCLC), DPPA2/4 co-expression associated with poorly differentiated tumours, impaired patient outcomes accelerated vivo xenograft tumour growth. Proteomic analyses dimerise...
Abstract A genetic map of Lolium has been produced using isozyme, restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers applied to a segregating family derived from an F 1 hybrid plant L. perenne × multiflorum provenance, crossed on doubled haploid perenne. total 106 markers, out 160 loci analysed, have ascribed seven linkage groups covering distance 692cM, Two these may be allocated chromosomes 2 6 the genome. The remaining unallocated majority...
Collagen I is a major tendon protein whose polypeptide chains are linked by covalent crosslinks. It unknown how the crosslinking contributes to mechanical properties of or whether changes in response stretching relaxation. Since their discovery, imine bonds within collagen have been recognized as being important both crosslink formation and structure. They often described acidic thermally labile, but no evidence available from direct measurements levels these contribute collagen. Here, we...
1. The aim of this study was to characterize the pharmacological profile GABA(B1)/GABA(B2) heterodimeric receptor expressed in Chinese hamster ovary (CHO) cells. We have compared binding affinity and functional activity for a series agonists antagonists. 2. chimeric G-protein, G(qi5), used couple activation increases intracellular calcium studies on Fluorimetric Imaging Plate Reader (FLIPR), using stable GABA(B1)/GABA(B2)/G(qi5) CHO cell line. [(3)H]-CGP-54626 radioligand membranes prepared...
How germinal centre (GC) B cells undergo rapid cell division while maintaining genome stability is poorly understood. Here, we show that the RNA-binding proteins ZFP36L1 and ZFP36L2 act downstream of antigen-sensing protect GC from replication stress by controlling a cycle-related RNA post-transcriptional regulon. safeguard faithful completion mitosis restraining expression CDK1 cyclin B1, whilst their activity through regulation p21-mediated negative feedback loop. In absence ZFP36L2,...
ABSTRACT Naïve epiblast cells in the embryo and pluripotent stem vitro undergo developmental progression to a formative state competent for lineage specification. During this transition, transcription factors chromatin are rewired encode new functional features. Here, we examine role of mitogen-activated protein kinase (ERK1/2) signalling transition. We show that primary consequence ERK activation mouse embryonic is elimination Nanog, precipitating breakdown naïve gene regulatory network....
Rac GTPases are required for neutrophil adhesion and migration, the effector responses that kill pathogens. These Rac-dependent functions impaired when neutrophils lack activators of Rac, Rac-GEFs from Prex, Vav, Dock families. In this study, we demonstrate Tiam1 is also expressed in neutrophils, governing focal complexes, actin cytoskeletal dynamics, polarisation, a manner depending on integrin ligand to which cells adhere. dispensable generation reactive oxygen species but mediates...
Scalp epidermoid cysts are a common tumor subtype in head, which usually exhibit hypodense on CT scans. Here, the authors report case of 50 years old man with mass at left frontal scalp hyperdense Subsequent histological analysis identified as an cyst.