A5101555482- Cancer Research and Treatments
- Virus-based gene therapy research
- Genetics, Aging, and Longevity in Model Organisms
- Circadian rhythm and melatonin
- CAR-T cell therapy research
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- Spaceflight effects on biology
- Wound Healing and Treatments
- Bacterial Infections and Vaccines
- Reproductive tract infections research
- S100 Proteins and Annexins
- Neuroblastoma Research and Treatments
- Natural product bioactivities and synthesis
- Ocular Surface and Contact Lens
- Anorectal Disease Treatments and Outcomes
- bioluminescence and chemiluminescence research
- Medical and Biological Ozone Research
- Herpesvirus Infections and Treatments
- Amoebic Infections and Treatments
- Ginseng Biological Effects and Applications
- Connective tissue disorders research
- Reproductive System and Pregnancy
- Oral microbiology and periodontitis research
- Spondyloarthritis Studies and Treatments
Yanshan University
2024
National Taiwan University
2011-2024
Memorial Sloan Kettering Cancer Center
2008-2023
Kettering University
2011-2023
California Institute of Technology
2023
National Central University
2020
Chi Mei Medical Center
2010-2019
Dayeh University
2016
Institute of Molecular Medicine
2014
Institute for Biomedicine
2013
Differing from the traditional view that power frequency electric field is always considered a negative phenomenon, here we report concept uses directly as an energy source to generate sensing signals. To demonstrate this, integrated bionic perception and transmission nerve device (BPTND) based on developed. The BPTND can effectively simulate sensory nervous systems by integrating perception, recognition, functions detect transmit positional information of mechanical stimulation. Results...
Although many genes have been implicated in the pathogenesis of common neurodegenerative diseases, genetic and cellular mechanisms that maintain neuronal integrity during normal aging remain elusive. Here we show Caenorhabditis elegans touch receptor cholinergic neurons display age-dependent morphological defects, including cytoskeletal disorganization, axon beading, defasciculation. Progression is regulated by DAF-2 DAF-16 signaling, which also modulate adult life span. Mutations disrupt...
Significance In this study, we identify nerve-released glial cell line-derived neurotrophic factor (GDNF) family receptor (GFR)α1 as a key that enhances perineural invasion (PNI) through GDNF-Ret proto-oncogene (RET) signaling. We demonstrate GFRα1 is released from nerves in soluble form and cooperates with secreted GDNF to activate cancer surface RET, activating downstream signaling, migration, PNI. These findings advance our understanding of the molecular mechanisms PNI define specific...
Abstract Perineural invasion (PNI) is an ominous event strongly linked to poor clinical outcome. Cells residing within peripheral nerves collaborate with cancer cells enable PNI, but the contributing conditions tumor microenvironment are not well understood. Here, we show that CCR2-expressing inflammatory monocytes (IM) preferentially recruited sites of where they differentiate into macrophages and potentiate nerve through a cathepsin B–mediated process. A series adoptive transfer...
Background: Progression of diabetes‐associated periodontal destruction and the roles advanced glycation end products (AGEs) are investigated. Methods: Diabetes was induced by streptozocotin injection, periodontitis via silk ligature placement with Porphyromonas gingivalis lipopolysaccharide injection in 64 Sprague‐Dawley rats for 7 to 21 days. The quality alveolar bone attachment loss (AL) were measured microcomputed tomography histology. Destruction profiles evaluated histology,...
Perineural invasion (PNI) by cancer cells is an ominous clinical event that associated with increased local recurrence and poor prognosis. Although radiation therapy (RT) may be delivered along the course of invaded nerve, mechanisms through which potentially control PNI remain undefined.An in vitro co-culture system dorsal root ganglia (DRG) pancreatic was used as a model PNI. An vivo murine sciatic nerve to study how RT or affects cancer.Cancer cell DRG partially dependent on secretion...
Anaplastic thyroid carcinoma (ATC) is a fatal disease with median survival of only 6 months. Novel therapies are needed to improve dismal outcomes.A mutated, replication-competent, vaccinia virus (GLV-1h68) has oncolytic effects on human ATC cell lines in vitro. We assessed the utility GLV-1h68 treating anaplastic cancer vivo.Athymic nude mice xenograft flank tumors ATCs (8505C and DRO90-1) were treated single intratumoral injection at low dose (5x10(5) plaque-forming unit), high (5x10(6) or...
Oncolytic viruses show promise for treating cancer. However, to assess therapeutic efficacy and potential toxicity, a noninvasive imaging modality is needed. This study aimed determine if insertion of the human sodium iodide symporter (hNIS) cDNA as marker non-invasive virotherapy alters replication oncolytic capability novel vaccinia virus, GLV-1h153.GLV-1h153 was modified from parental virus GLV-1h68 carry hNIS via homologous recombination. GLV-1h153 tested against pancreatic cancer cell...
Background: Diabetes is known to impair wound healing and deteriorate the periodontal condition. There limited information about patterns events associated with repair. In this study, we evaluate dynamics of repair using micro‐computed tomography (microCT) immunohistochemistry. Methods: Thirty‐six male rats were used, diabetes was induced by streptozotocin. The maxillary first molars extracted, a tooth‐associated osseous defect created in extraction area. Animals sacrificed after 7, 14, 21...
Significance Structural remodeling of neurons after insults to the nervous system includes retraction dysfunctional synaptic branches and growth primary neurites or new collateral branches. We find that genetic disruption neuronal microtubules in Caenorhabditis elegans triggered structural through RHGF-1/RhoGEF, which is normally associated with inhibited by microtubules. A conserved dual leucine zipper kinase, DLK-1, was activated RHGF-1-dependent signaling, DLK-1 transported from distal...
We investigated the therapeutic efficacy of a replication-competent oncolytic vaccinia virus, GLV-1h153, carrying human sodium iodide symporter (hNIS), in combination with radioiodine an orthotopic triple-negative breast cancer (TNBC) murine model. In vitro viral infection was confirmed by immunoblotting and uptake assays. Orthotopic xenografts (MDA-MB-231 cells) received intratumoral injection GLV-1h153 or PBS. One week after injection, were randomized into 4 treatment groups: alone, 131I...
The heterogeneity and multigenetic nature of nervous system aging make modeling it a formidable task in mammalian species. powerful genetics, simple anatomy short life span the nematode Caenorhabditis elegans offer unique advantages unraveling molecular genetic network that regulates integrity neuronal structures functions during aging. In this review, we first summarize recent breakthroughs morphological functional characterization C. Age-associated changes include age-dependent neurite...
Spatial arrangement of neurite branching is instructed by both attractive and repulsive cues. Here we show that in C. elegans, the Wnt family secreted glycoproteins specify sites PLM mechanosensory neurons. Wnts function through MIG-1/Frizzled planar cell polarity protein (PCP) VANG-1/Strabismus/Vangl2 to restrict formation F-actin patches, which mark nascent neurites. We find VANG-1 promotes signaling facilitating Frizzled endocytosis genetically acts a common pathway with arr-1/β-arrestin,...
We used reduced slice reparations to study ASIC-like currents in the rat central clock suprachiasmatic nucleus (SCN). In SCN preparations, a drop of extracellular pH evoked desensitizing inward current excite neurones fire at higher rates. Under voltage-clamped conditions, all responded 5 s step 6.4 with an that decayed average time constant 1.2 10% peak end step. The was blocked by amiloride IC(50) 14 microm and carried mainly Na(+), suggesting origin channels. were sensitive neutral pH,...
To assess therapeutic response and potential toxicity of oncolytic virotherapy, a noninvasive, deep-tissue imaging modality is needed. This study aimed to the feasibility, parameters, determining factors serial long-term monitoring virotherapy pancreatic cancer xenografts treated with vaccinia virus carrying human sodium iodide symporter GLV-1h153. <b>Methods:</b> Pancreatic (PANC-1) in nude mice were systemically or intratumorally GLV-1h153 serially imaged using <sup>124</sup>I PET at 1, 2,...
Introduction Oncolytic viruses show promise for treating cancer. However, to assess therapy and potential toxicity, a noninvasive imaging modality is needed. This study aims determine the in vivo biodistribution, timing characteristics of vaccinia virus, GLV-1h153, encoding human sodium iodide symporter (hNIS. Methods GLV-1h153 was modified from GLV-1h68 encode hNIS gene. Timing cellular uptake radioiodide 131I pancreatic carcinoma cells PANC-1 assessed using radiouptake assays. Viral...
Neuronal cargos are differentially targeted to either axons or dendrites, and this polarized cargo targeting critically depends on the interaction between microtubules molecular motors. From a forward mutagenesis screen, we identified gain-of-function mutation in C. elegans α-tubulin gene mec-12 that triggered synaptic vesicle mistargeting, neurite swelling neurodegeneration touch receptor neurons. This missense replaced an absolutely conserved glycine H12 helix with glutamic acid, resulting...
To examine the therapeutic effects of an attenuated, replication-competent vaccinia virus (GLV-1h68) against a panel 5 human salivary gland carcinoma cell lines.The susceptibility lines to infection and oncolysis by GLV-1h68 was assessed in vitro vivo.All were susceptible viral infection, transgene expression, cytotoxic reactions. Three exquisitely sensitive very low doses GLV-1h68. Orthotopic parotid tumors exhibited more aggressive behavior compared with flank tumors. A single intratumoral...