A5086711705- Genetics, Aging, and Longevity in Model Organisms
- Circadian rhythm and melatonin
- Neurobiology and Insect Physiology Research
- Pluripotent Stem Cells Research
- Biochemical Acid Research Studies
- Neuroendocrine regulation and behavior
- CRISPR and Genetic Engineering
- Mitochondrial Function and Pathology
- Microtubule and mitosis dynamics
- Spaceflight effects on biology
- Mechanisms of cancer metastasis
- DNA Repair Mechanisms
- Insect and Arachnid Ecology and Behavior
- Gut microbiota and health
- Physiological and biochemical adaptations
- Insect Utilization and Effects
- Digestive system and related health
California Institute of Technology
2018-2024
Abstract Null mutants are essential for analyzing gene function. Here, we describe a simple and efficient method to generate Caenorhabditis elegans null using CRISPR/Cas9 short single stranded DNA oligo repair templates insert universal 43-nucleotide-long knock-in cassette (STOP-IN) into the early exons of target genes. This STOP-IN has stop codons in all three reading frames leads frameshifts, which will putative mutations regardless frame insertion position exons. The also contains an...
The nematode intestine is the primary site for nutrient uptake and storage as well synthesis of biomolecules; lysosome-related organelles known gut granules are important many these functions. Aspects biology not understood, including export nutrients it imports molecules synthesizes, complete functions protein content granules. Here, we report a mass spectrometry (MS)–based proteomic analysis Caenorhabditis elegans its Overall, identified approximately 5,000 proteins each in gonad showed...
Signaling molecules derived from attachment of diverse metabolic building blocks to ascarosides play a central role in the life history C. elegans and other nematodes; however, many aspects their biogenesis remain unclear. Using comparative metabolomics, we show that pathway mediating formation intestinal lysosome-related organelles (LROs) is required for biosynthesis most modular as well previously undescribed glucosides. Similar ascarosides, glucosides are highly selective assembly...
Abstract Neuropeptides are evolutionarily conserved modulators of many aspects animal behavior and physiology, expand the repertoire processes that can be controlled by a limited number neurons. Deciphering neuropeptidergic codes govern distinct requires systematic functional analyses neuropeptides their cognate receptors. Even in well-studied model organisms like Caenorhabditis elegans, however, such efforts have been precluded lack mutant reagents. Here, we generated screened 21 C. elegans...
Abstract Decisions made over long time scales, such as life cycle decisions, require coordinated interplay between sensory perception and sustained gene expression. The Caenorhabditis elegans dauer (or diapause) exit developmental decision requires integration of population density food availability to induce an all-or-nothing organismal-wide response, but the mechanism by which this occurs remains unknown. Here, we demonstrate how ASJ chemosensory neurons, known be critical for exit,...
Decisions made over long time scales, such as life cycle decisions, require coordinated interplay between sensory perception and sustained gene expression. The Caenorhabditis elegans dauer (or diapause) exit developmental decision requires integration of population density food availability to induce an all-or-nothing organismal-wide response, but the mechanism by which this occurs remains unknown. Here, we demonstrate how Amphid Single Cilium J (ASJ) chemosensory neurons, known be critical...
Cell adhesion plays essential roles in almost every aspect of metazoan biology. LINKIN (Human: ITFG1, Caenorhabditis elegans: lnkn-1) is a conserved transmembrane protein that has been identified to be necessary for tissue integrity during migration. In C. elegans, loss lnkn-1 results the detachment lead migratory cell from rest developing male gonad. Previously, three interactors ITFG1/lnkn-1 - RUVBL1/ruvb-1, RUVBL2/ruvb-2, and alpha-tubulin were by immunoprecipitation-mass spectrometry...
Abstract Chemosensation plays a role in the behaviors and life cycles of numerous organisms, including nematodes. Many guilds nematodes exist, ranging from free-living Caenorhabditis elegans to various parasitic species such as entomopathogenic (EPNs), which are parasites insects. Despite ecological differences, previous research has shown that both EPNs C. respond prenol (3-methyl-2-buten-1-ol), an odor associated with EPN infections. However, it is unclear how responds prenol. By utilizing...
Abstract Signaling molecules derived from attachment of diverse metabolic building blocks to ascarosides play a central role in the life history C. elegans and other nematodes; however, many aspects their biogenesis remain unclear. Using comparative metabolomics, we show that pathway mediating formation intestinal lysosome-related organelles (LROs) is required for biosynthesis most modular as well previously undescribed glucosides. Similar ascarosides, glucosides are highly selective...
Abstract Null mutants are essential for analyzing gene function. Here, we describe a simple and efficient method to generate Caenorhabditis elegans null using CRISPR/Cas9 short single stranded DNA oligo repair templates insert universal 43-nucleotide-long stop knock-in (STOP-IN) cassette into the early exons of target genes. This has codons in all three reading frames leads frameshifts, which will putative mutations regardless frame insertion position exons. The STOP-IN also contains an...
Not much is known about FAM136A, a human gene that may be involved in Meniere's disease and conserved throughout animals. To understand the function of
Abstract The nematode intestine is the primary site for nutrient uptake and storage as well synthesis of biomolecules; lysosome-related organelles known gut granules are important many these functions. Aspects biology not understood, including export nutrients it imports molecules synthesizes, complete functions protein content granules. Here, we report a mass spectrometry-based proteomic analysis Caenorhabditis elegans its Overall, identified approximately 5,000 proteins each in gonad...
SUMMARY Animals integrate external stimuli to shape their physiological responses throughout development. In adverse environments, Caenorhabditis elegans larvae can enter a stress-resistant diapause state with arrested metabolism and reproductive physiology. Amphid sensory neurons feed into both rapid chemotactic short-term foraging mode decisions, mediated by amphid premotor interneurons, as well the long-term decision. We identify interneurons that pheromone cues propagate this information...