A5100627894- CRISPR and Genetic Engineering
- Genetics, Aging, and Longevity in Model Organisms
- RNA and protein synthesis mechanisms
- MicroRNA in disease regulation
- RNA modifications and cancer
- Molecular Biology Techniques and Applications
- Photoreceptor and optogenetics research
- Circadian rhythm and melatonin
- Pluripotent Stem Cells Research
- Herpesvirus Infections and Treatments
- Mosquito-borne diseases and control
- Parvovirus B19 Infection Studies
- Biosensors and Analytical Detection
- Retinal Development and Disorders
- Genomics and Phylogenetic Studies
- Genomics and Chromatin Dynamics
- Advanced Chemical Sensor Technologies
- Microbial Metabolic Engineering and Bioproduction
- Cancer Genomics and Diagnostics
- Identification and Quantification in Food
- HIV Research and Treatment
- Epigenetics and DNA Methylation
- Gene Regulatory Network Analysis
- Allergic Rhinitis and Sensitization
- Neurobiology and Insect Physiology Research
California Institute of Technology
2016-2023
Harvey Mudd College
2020
Howard Hughes Medical Institute
2016
Johns Hopkins University
2011-2014
Johns Hopkins Medicine
2011-2013
Abcam (United States)
2007
Designer Chromosome One of the ultimate aims synthetic biology is to build designer organisms from ground up. Rapid advances in DNA synthesis has allowed assembly complete bacterial genomes. Eukaryotic organisms, with their generally much larger and more complex genomes, present an additional challenge biologists. Annaluru et al. (p. 55 , published online 27 March) designed a eukaryotic chromosome based on yeast III. The chromosome, shorn destabilizing transfer RNA genes transposons, ∼14%...
Abstract Null mutants are essential for analyzing gene function. Here, we describe a simple and efficient method to generate Caenorhabditis elegans null using CRISPR/Cas9 short single stranded DNA oligo repair templates insert universal 43-nucleotide-long knock-in cassette (STOP-IN) into the early exons of target genes. This STOP-IN has stop codons in all three reading frames leads frameshifts, which will putative mutations regardless frame insertion position exons. The also contains an...
Plasma microRNAs (miRNAs) are modulated during disease and emerging biomarkers; they have not been characterized in HIV infection. Using our macaque/simian immunodeficiency virus (SIV) model of HIV, we sought to identify a plasma miRNA profile acute lentiviral infection, evaluate its relationship with known cellular viral determinants lentivirus-associated central nervous system (CNS) disease, explore the potential miRNAs predict CNS disease.Plasma samples were obtained before inoculation 10...
Significance Discovering where a gene acts, when it is required, and the consequence of its perturbation are essential for understanding function. These tasks require genetic tools that allow precise control expression at will. In this paper, we combine split intein strategy bipartite system to develop refined method controlling transgene in Caenorhabditis elegans . The new specifies only cells two distinct promoters active, allowing spatiotemporal control. Our work provides C. community...
Abstract Background The RNA-binding protein tristetraprolin (TTP) participates in normal post-transcriptional control of cytokine and chemokine gene expression, dysregulation which contributes to the HIV-associated neurocognitive disorders. Transcriptional regulation TTP has been described, including by microRNA-29a. In simian immunodeficiency virus (SIV) model HIV CNS disease, cytokine/chemokine expression coincides with end acute phase infection. This is lost during progression disease....
Animals integrate sensory information from the environment and display various behaviors in response to external stimuli. In Caenorhabditis elegans hermaphrodites, 33 types of neurons are responsible for chemosensation, olfaction, mechanosensation. However, functional roles all have not been systematically studied due lack facile genetic accessibility. A bipartite cGAL-UAS system has previously developed study tissue- or cell-specific functions C. . Here, we report a toolkit new cGAL drivers...
Abstract Traditional pollen preparation techniques provide clear residues for identification; however, such methods are time‐consuming, requiring repeated centrifugation, heating, and digestion with high‐concentration hazardous chemicals. Tobacco leaves can effectively trap environmental due to hairy surface terpene‐rich exudates. A new tobacco sample processing method was developed by using different extraction chemistry surfactant. Marlboro Gold cigarettes were employed as model samples...
Abstract Null mutants are essential for analyzing gene function. Here, we describe a simple and efficient method to generate Caenorhabditis elegans null using CRISPR/Cas9 short single stranded DNA oligo repair templates insert universal 43-nucleotide-long stop knock-in (STOP-IN) cassette into the early exons of target genes. This has codons in all three reading frames leads frameshifts, which will putative mutations regardless frame insertion position exons. The STOP-IN also contains an...
Abstract High throughput sequencing technologies open up a new dimension in cancer genomics by enabling the characterization of genomes at base-pair resolution. To date, majority large-scale projects rely on fresh-frozen biospecimens their studies, which are difficult to obtain and often lack long-term clinical data. Unlike samples, archived formalin-fixed paraffin-embedded (FFPE) tissues more readily accessible, associated with known outcomes complete annotations. However, library...