A5102990753- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Fungal and yeast genetics research
- Yeasts and Rust Fungi Studies
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Effects of Radiation Exposure
- Fermentation and Sensory Analysis
- Microtubule and mitosis dynamics
- Antifungal resistance and susceptibility
- Genetics, Bioinformatics, and Biomedical Research
- Plant Disease Resistance and Genetics
- Genomics and Phylogenetic Studies
- Nuclear Physics and Applications
- DNA Repair Mechanisms
- RNA Research and Splicing
- Biomedical and Engineering Education
- Plant Molecular Biology Research
- Probiotics and Fermented Foods
- Protist diversity and phylogeny
- Infectious Disease Case Reports and Treatments
- Plant Reproductive Biology
- Microbial Community Ecology and Physiology
- Boron Compounds in Chemistry
- Graphite, nuclear technology, radiation studies
Centre National de la Recherche Scientifique
2008-2024
Institut Curie
2023-2024
Université Paris Sciences et Lettres
2023-2024
Muséum national d'Histoire naturelle
2024
Massachusetts Institute of Technology
2024
Sorbonne Université
2004-2024
Dynamique du noyau
2023-2024
Université de Lorraine
2021
Institut Pasteur
2008-2017
Department of Genomes & Genetics
2017
Designer Chromosome One of the ultimate aims synthetic biology is to build designer organisms from ground up. Rapid advances in DNA synthesis has allowed assembly complete bacterial genomes. Eukaryotic organisms, with their generally much larger and more complex genomes, present an additional challenge biologists. Annaluru et al. (p. 55 , published online 27 March) designed a eukaryotic chromosome based on yeast III. The chromosome, shorn destabilizing transfer RNA genes transposons, ∼14%...
Duplication and segregation of chromosomes involves dynamic reorganization their internal structure by conserved architectural proteins, including the structural maintenance (SMC) complexes cohesin condensin. Despite active investigation roles these factors, a genome-wide view chromosome architecture at both small large scale during cell division is still missing. Here, we report first comprehensive 4D analysis higher-order organization Saccharomyces cerevisiae genome throughout cycle...
Although the design of synthetic yeast genome Sc2.0 is highly conservative with respect to gene content, deletion several classes repeated sequences and introduction thousands designer changes may affect organization potentially alter cellular functions. We report here Hi-C-determined three-dimensional (3D) conformations chromosomes. The absence repeats leads a smoother contact pattern more precisely tractable chromosome conformations, large-scale genomic globally unaffected by presence...
ABSTRACT The genome of the type strain Candida glabrata (CBS138, ATCC 2001) contains homologs most genes involved in mating Saccharomyces cerevisiae , starting with pheromone and receptor genes. Only haploid cells are ever isolated, but C. strains both types commonly found, being MAT α other strains, such as BG2, a . No sexual cycle has been documented for this species. In order to understand which steps pathway defective, we have analyzed expression some key well production pheromones...
The first page of this article is displayed as the abstract.
In order to shed light on its basic biology, we initiated a population genetic analysis of Candida glabrata, an emerging pathogenic yeast with no sexual stage yet recognized. A worldwide collection clinical strains was subjected using variable number tandem repeats (VNTR) at nine loci. The clustering obtained this method congruent that sequence polymorphism the NMT1 gene, locus previously proposed for lineage assignment. Linkage disequilibrium supported hypothesis mainly clonal reproduction....
Genome sequencing and assembly of the photosynthetic picoeukaryotic Picochlorum sp. SENEW3 revealed a compact genome with reduced gene set, few repetitive sequences, an organized Rabl-like chromatin structure. Hi-C chromosome conformation capture evidence possible chromosomal translocations, as well putative centromere locations. Maintenance relatively selenoproteins, compared to similarly sized marine picoprasinophytes Mamiellales, broad halotolerance others in Trebouxiophyceae, suggests...
<title>Abstract</title> The hallmarks of chromosome organization in multicellular eukaryotes are territories (CT), chromatin compartments, and insulated domains, including topologically associated domains (TADs). Yet, most these elements derived from analyses a limited set model organisms, while large eukaryotic groups, insects, remain mostly unexplored. Here we combine Hi-C, biophysical modeling, microscopy to characterize the 3D genome architecture silkmoth, Bombyx mori. In contrast other...
Abstract To ensure the proper transmission of genetic information, DNA molecules must be faithfully duplicated and segregated. These processes involve dynamic modifications chromosomes internal structure to promote their individualization, as well global repositioning into daughter cells (Guacci et al., 1994; Kleckner 2014; Mizuguchi 2014). In eukaryotes, these events are regulated by conserved architectural proteins, such structural maintenance (SMC i.e. cohesin condensin) complexes (Aragon...
The main purpose of this contribution is to describe the new features latest version 7.4 CONtinuous-Time System IDentification (CONTSID) toolbox for Matlab. addition a Graphical User Interface (GUI), which allows user in friendly and easy way perform data analysis, model parameter estimation as well validation. recent additions MISO time-delay transfer function identification are also briefly introduced.
Abstract The Elizabeth W. Jones Award for Excellence in Education recognizes individuals or groups who have had significant, sustained impact on genetics education at any level, from K-12 through graduate school and beyond. 2024 recipient Jef Boeke considers himself a geneticist turned engineer. He transformed his landmark synthetic yeast genome project into research-heavy teaching course, revolutionizing molecular biology education. Build-A-Genome course was developed to teach students...