A5026641587- Genetics, Aging, and Longevity in Model Organisms
- Parasite Biology and Host Interactions
- Parasites and Host Interactions
- Evolution and Genetic Dynamics
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Bioinformatics and Genomic Networks
- Helminth infection and control
- Circadian rhythm and melatonin
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Enzyme Structure and Function
- Mitochondrial Function and Pathology
- Nematode management and characterization studies
- Photosynthetic Processes and Mechanisms
- Mycorrhizal Fungi and Plant Interactions
- Plant Molecular Biology Research
- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
- Fungal Biology and Applications
- Cervical Cancer and HPV Research
- RNA Research and Splicing
- Insect and Arachnid Ecology and Behavior
- Virus-based gene therapy research
- Insect Resistance and Genetics
Cornell University
2016-2025
The University of Melbourne
2013-2023
California Institute of Technology
2004-2016
Howard Hughes Medical Institute
1993-2016
Pediatrics and Genetics
2016
Washington University in St. Louis
2009-2011
Wellcome Sanger Institute
2007
Cold Spring Harbor Laboratory
2007
Stanford University
2003
Columbia University
1999
The Gene Ontology (GO) project (http://www. geneontology.org/) provides structured, controlled vocabularies and classifications that cover several domains of molecular cellular biology are freely available for community use in the annotation genes, gene products sequences. Many model organism databases genome groups GO contribute their sets to resource. database integrates contributed annotations full access this information formats. Members Consortium continually work collectively,...
WormBase (http://www.wormbase.org) is a central data repository for nematode biology. Initially created as service to the Caenorhabditis elegans research field, has evolved into powerful tool in its own right. In past 2 years, we expanded include complete genomic sequence, gene predictions and orthology assignments from range of related nematodes. This comparative enrich C. with improved better understanding function. turn, they bring wealth experimental knowledge other systems medical...
<ns3:p>The khmer package is a freely available software library for working efficiently with fixed length DNA words, or k-mers. provides implementations of probabilistic k-mer counting data structure, compressible De Bruijn graph representation, partitioning, and digital normalization. implemented in C++ Python, under the BSD license at <ns3:ext-link xmlns:ns4="http://www.w3.org/1999/xlink" ext-link-type="uri"...
The chromosomal locations of cellular sequences flanking integrated papillomavirus DNA in four cervical carcinoma cell lines and a primary have been determined. two human (HPV) 16 derived from the tumor were localized to chromosome regions 20pter----20q13 3p25----3qter, that also contain protooncogenes c-src-1 c-raf-1, respectively. HPV integration site SiHa carcinoma-derived line is region 13q14----13q32. 18 SW756 cells 12 but not closely linked Ki-ras2 gene. Finally, lines, HeLa C4-I, 8,...
The genome of the common roundworm Ascaris suum, a major parasite in many parts world, has now been sequenced. Analyses reveal genes that encode peptidases linked to penetration and degradation host tissues, as well molecules likely modulate or evade immune responses. availability this sequence should contribute towards development new therapeutic interventions against ascariasis other nematode infections. Parasitic diseases have devastating, long-term impact on human health, welfare food...
The barber's pole worm, Haemonchus contortus, is one of the most economically important parasites small ruminants worldwide. Although this parasite can be controlled using anthelmintic drugs, resistance against drugs in common use has become a widespread problem. We provide draft genome and transcriptomes all key developmental stages H. contortus to support biological biotechnological research areas related parasites. 320 Mb size encodes 23,610 protein-coding genes. On fundamental level, we...
We have cloned Calx , a gene that encodes Na-Ca exchanger of Drosophila melanogaster . two repeated motifs, -α and -β, overlap domains required for activity regulation. has multiple transcripts in adults, including at least one expressed the retina. The genomic locus comprises ≥35 kb between Atpα rudimentary-like genes chromosomal region 93B. In Xenopus oocytes, microinjected cRNA induces calcium uptake like its homolog, 3Na + -1Ca 2+ mammalian heart. Implications motifs mechanism exchange...
Since its release in 2000, WormBase (http://www.wormbase.org) has grown from a small resource focusing on single species and serving dedicated research community, to one now spanning 15 essential the broader biomedical agricultural fields. To enhance rate of curation, we have automated identification key data scientific literature use similar methodology for extraction. ease access data, are collaborating with journals link entities publications their report pages at WormBase. facilitate...
Caenorhabditis elegans was the first multicellular eukaryotic genome sequenced to apparent completion. Although this assembly employed a standard C. strain (N2), it used sequence data from several laboratories, with DNA propagated in bacteria and yeast. Thus, N2 has many differences any available today. To provide more accurate genome, we performed long-read of VC2010, modern derived N2. Our VC2010 99.98% identity but an additional 1.8 Mb including tandem repeat expansions duplications. For...
Examining the consequences of selfing The Caenorhabditis genus nematodes includes a mix closely related outcrossing and self-fertilizing (selfing) species. Genome size differs widely among these different Yin et al. generated genome assembly for nematode C. nigoni compared it with that its close relative, briggsae. briggsae has experienced substantial decrease in since two species' recent divergence. underlying causes this difference appear to involve protein-coding genes changes other types...
Trichuris (whipworm) infects 1 billion people worldwide and causes a disease (trichuriasis) that results in major socioeconomic losses both humans pigs. Trichuriasis relates to an inflammation of the large intestine manifested bloody diarrhea, chronic can cause malnourishment stunting children. Paradoxically, pigs has shown substantial promise as treatment for human autoimmune disorders, including inflammatory bowel (IBD) multiple sclerosis. Here we report whole-genome sequencing at...
To study the molecular basis for predator-prey coevolution, we investigated how Caenorhabditis elegans responds to predatory fungus Arthrobotrys oligospora. C. and other nematodes were attracted volatile compounds produced by A. Gas-chromatographic mass-spectral analyses of oligospora-derived metabolites identified several odors mimicking food cues attractive nematodes. One compound, methyl 3-methyl-2-butenoate (MMB) additionally triggered strong sex- stage-specific attraction in species....
Erich Schwarz and colleagues report whole-genome sequencing of the zoonotic hookworm Ancylostoma ceylanicum. They also analyze transcriptome through course infection in golden hamsters identify groups genes showing differential regulation across different stages. Hookworms infect over 400 million people, stunting impoverishing them1,2,3. Sequencing genomes finding which they express during should help devising new drugs or vaccines against hookworms4,5. Unlike other hookworms, ceylanicum...
Summary Mutants (glk) of Streptomyces coelicolor A3(2) that are resistant to the non‐utilizable glucose analogue 2‐deoxyglucose deficient in kinase activity, defective repression, and usually unable utilize glucose. A 2.9 kb Bcll fragment, previously shown restore a wild‐type phenotype glk deletion mutant lacks entire segment, contains two complete open reading frames would encode proteins 20.1 kDa (ORF2) 33.1 (ORF3). ORF3 is transcribed from its own promoter, also promoter initiates...
In our hybrid-plasmid reconstruction analysis of lambda (lambdoid) DNA signal structures involved in phage replication, we have detected a dual system alternatingly able to initiate first primer-RNA synthesis. Both them--the major, primase-dependent ori and the minor usually suppressed, RNA-polymerase-dependent oop system--act conjunction with common structure for inception It appears that situations such as this, where one has deal existence regular well backup systems serving same...
Efficient sequencing of animal and plant genomes by next-generation technology should allow many neglected organisms biological medical importance to be better understood. As a test case, we have assembled draft genome Caenorhabditis sp. 3 PS1010 through combination direct scaffolding with RNA-seq. We first sequenced genomic DNA mixed-stage cDNA using paired 75-nt reads from an Illumina GAII. A set 230 million yielded 80-Mb assembly, supercontig N50 5.0 kb, covering 90% 429 kb previously...
Abstract During development, cells not only adopt specialized identities but also maintain those identities. Endoreduplication is thought to cell identity. High concentrations of ARABIDOPSIS THALIANA MERISTEM LAYER1 (ATML1) specify giant identity and induce endoreduplication in sepals. How different ATML1 can remains unclear. Here, we show that high the biosynthesis both long-chain very fatty acids (LCFAs/VLCFAs), these are required for maintenance Inhibition VLCFA causes endoreduplicated...