A5057231025- CRISPR and Genetic Engineering
- Genetics, Aging, and Longevity in Model Organisms
- MicroRNA in disease regulation
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- Circular RNAs in diseases
- RNA Interference and Gene Delivery
- Genomics and Chromatin Dynamics
- Bacteriophages and microbial interactions
- Pluripotent Stem Cells Research
- Muscle Physiology and Disorders
- RNA modifications and cancer
- RNA regulation and disease
- Monoclonal and Polyclonal Antibodies Research
- Animal Genetics and Reproduction
- Chromosomal and Genetic Variations
- HIV Research and Treatment
- Plant Virus Research Studies
- Genomics and Phylogenetic Studies
- Photosynthetic Processes and Mechanisms
- Evolution and Genetic Dynamics
- Viral Infectious Diseases and Gene Expression in Insects
- Molecular Biology Techniques and Applications
- DNA Repair Mechanisms
Stanford University
2016-2025
Enzo Life Sciences (United States)
2019
ORCID
2018
Pediatrics and Genetics
2008-2016
Stanford Medicine
2007-2016
Sustainable Sciences Institute
2015
University of California, Berkeley
2015
Washington University in St. Louis
2014
Universität Hamburg
2011
Berlin-Brandenburger Centrum für Regenerative Therapien
2011
We have developed a cell-free system for studying the synthesis of mRNA in mammalian cells. The consists dialyzed and concentrated whole-cell extract derived from HeLa cells, small molecules cofactors needed transcription, exogenously added DNA. Accurate transcription by RNA polymerase II is entirely dependent upon addition promoter-containing eukaryotic At optimal DNA concentrations, initiation adenovirus serotype 2 late promoter readily detectable, specific transcripts over 4000...
Short interfering RNAs (siRNAs) are double-stranded of ≈21–25 nucleotides that have been shown to function as key intermediaries in triggering sequence-specific RNA degradation during posttranscriptional gene silencing plants and interference invertebrates. siRNAs a characteristic structure, with 5′-phosphate/3′-hydroxyl ends 2-base 3′ overhang on each strand the duplex. In this study, we present data synthetic can induce gene-specific inhibition expression Caenorhabditis elegans cell lines...
Abstract Facilitated by recent advances using CRISPR/Cas9, genome editing technologies now permit custom genetic modifications in a wide variety of organisms. Ideally, modified animals could be both efficiently made and easily identified with minimal initial screening without introducing exogenous sequence at the locus interest or marker mutations elsewhere. To this end, we describe coconversion strategy, CRISPR/Cas9 which for dominant phenotypic oligonucleotide-templated conversion event...
Natural genes and proteins often contain tandemly repeated sequence motifs that dramatically increase physiological specificity activity. Given the selective value of such repeats, it is likely several different mechanisms have been responsible for their generation. One mechanism has shown to generate relatively long tandem repeats (in kilobase range) rolling circle replication. In this communication, we demonstrate synthesis in a simple enzymatic system can produce monomers as short 34 bp....
Competition between mammalian RNAi-related gene silencing pathways is well documented. It therefore important to identify all classes of small RNAs determine their relationship with RNAi and how they affect each other functionally. Here, we two types 5′-phosphate, 3′-hydroxylated human tRNA-derived (tsRNAs). tsRNAs differ from microRNAs in being essentially restricted the cytoplasm associating Argonaute proteins, but not MOV10. The first type belongs a previously predicted Dicer-dependent...
RNA interference (RNAi) is a phylogenetically widespread gene-silencing process triggered by double-stranded RNA. In plants and Caenorhabditis elegans, two distinct populations of small RNAs have been proposed to participate in RNAi: "Primary siRNAs" (derived from DICER nuclease-mediated cleavage the original trigger) "secondary [additional whose synthesis requires an RNA-directed polymerase (RdRP)]. Analyzing associated with ongoing RNAi C. we found that secondary siRNAs constitute vast...
Using the massively parallel technique of sequencing by oligonucleotide ligation and detection (SOLiD; Applied Biosystems), we have assessed in vivo positions more than 44 million putative nucleosome cores multicellular genetic model organism Caenorhabditis elegans. These analyses provide a global view chromatin architecture animal at extremely high density resolution. While observe some degree reproducible positioning throughout genome our mixed stage population animals, note that major...
Introduction of exogenous double-stranded RNA (dsRNA) into Caenorhabditis elegans has been shown to specifically and potently disrupt the activity genes containing homologous sequences. In this study we present evidence that primary interference effects dsRNA are post-transcriptional. First, examined DNA sequence after dsRNA-mediated found no for alterations. Second, with upstream gene in a polar operon had effect on downstream gene; finding argues against an initiation or elongation...
In screening for embryonic-lethal mutations in Caenorhabditis elegans, we defined an essential gene (let-858) that encodes a nuclear protein rich acidic and basic residues. We have named this product nucampholin. Closely homologous sequences yeast, plants, mammals demonstrate strong evolutionary conservation eukaryotes. Nucampholin resides all nuclei of C. elegans is early development differentiating tissue. Antisense-mediated depletion LET-858 activity embryos causes lethal phenotype...
Abstract Four Caenorhabditis elegans genes encode muscle-type specific myosin heavy chain isoforms: myo-1 and myo-2 are expressed in the pharyngeal muscles; unc-54 myo-3 body wall muscles. We have used transformation-rescue lacZ fusion assays to determine sequence requirements for regulated gene expression during development. Multiple tissue-specific activation elements present all four genes. For each of genes, sequences upstream coding region promoters, as shown by their ability drive a...
Abstract Recent studies suggest that knowledge of differential expression microRNAs (miRNA) in cancer may have substantial diagnostic and prognostic value. Here, we use a direct sequencing method to characterize the profiles miRNAs other small RNA segments for six human cervical carcinoma cell lines five normal samples. Of 166 expressed cervix lines, observed significant variation between two groups. To further show biological relevance our findings, examined level significantly varying...