A5083081321- Plant Reproductive Biology
- Plant Molecular Biology Research
- Ionic liquids properties and applications
- Electronic and Structural Properties of Oxides
- Semiconductor materials and devices
- Tree Root and Stability Studies
- Cellular transport and secretion
- Polysaccharides and Plant Cell Walls
- Cellular Mechanics and Interactions
- Lipid Membrane Structure and Behavior
- Advanced Materials and Mechanics
- Force Microscopy Techniques and Applications
- Polymer-Based Agricultural Enhancements
- Biocrusts and Microbial Ecology
- Molecular Junctions and Nanostructures
- Light effects on plants
- Skin and Cellular Biology Research
- Plant Physiology and Cultivation Studies
- Modular Robots and Swarm Intelligence
- Photosynthetic Processes and Mechanisms
- Turfgrass Adaptation and Management
- Bacterial biofilms and quorum sensing
- Calcium signaling and nucleotide metabolism
- Analytical Chemistry and Sensors
- Electrochemical Analysis and Applications
Université Claude Bernard Lyon 1
2019-2024
Laboratoire de Reproduction et Développement des Plantes
2019-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
École Normale Supérieure de Lyon
2019-2024
Centre National de la Recherche Scientifique
2017-2024
Inserm
2017-2024
Lyon College
2019
University of Milan
2009-2018
Université de Lille
2017-2018
Center for Infection and Immunity of Lille
2017-2018
We present a procedure that allows reliable determination of the elastic (Young’s) modulus soft samples, including living cells, by atomic force microscopy (AFM). The standardized nanomechanical AFM (SNAP) ensures precise adjustment optical lever system, prerequisite for all kinds spectroscopy methods, to obtain values independent instrument, laboratory and operator. Measurements hydrogel samples with well-defined using different AFMs revealed uncertainties in deflection sensitivity...
We report the direct observation of solidlike ordering at room temperature thin films [Bmim][NTf2] ionic liquid on mica, amorphous silica, and oxidized Si(110). A statistical quantitative analysis atomic force microscopy topographies shows that these surfaces forms layered structures, characterized by a perpendicular structural periodicity approximately 0.6 nm. Remarkably, even highest up to 50 nm high, behave against AFM probe. Conversely, highly oriented pyrolitic graphite nanometer-sized,...
Abstract The epidermis is a stratified epithelium that forms the outer layer of skin. It composed primarily keratinocytes and constantly renewed by proliferation stem cells their progeny undergo terminal differentiation as they leave basal migrate to skin surface. Basal rest on basement membrane an extracellular matrix controls fate via integrin‐mediated focal adhesions hemidesmosomes which are critical elements epidermal barrier promote its regenerative capabilities. distribution with...
Successful fertilization in angiosperms depends on the proper trajectory of pollen tubes through pistil tissues to reach ovules. Pollen first grow within cell wall papilla cells, applying pressure cell. Mechanical forces are known play a major role plant shape by controlling orientation cortical microtubules (CMTs), which turn mediate deposition cellulose microfibrils (CMFs). Here, combining imaging, genetic and chemical approaches, we show that isotropic reorientation CMTs CMFs aged Col-0...
Polar cell growth is a fundamental process across organisms, yet its coordination with nuclear movement and cytoskeleton dynamics remains underexplored. Focusing on Arabidopsis thaliana root hairs, we investigate these processes using high-resolution live imaging within microfluidics-based experiments. By incorporating data cytoskeletal dynamics, positioning, tip into mathematical model, analyse how their interactions shape the different phases that reveal for first time in this study....
Abstract Plants generate motion by absorbing and releasing water. Many Asteraceae plants, such as the dandelion, have a hairy pappus that can close depending on moisture levels to modify dispersal. Here we demonstrate relationship between structure function of underlying hygroscopic actuator. By investigating properties actuator cell walls, identify mechanism which dandelion closes. We developed structural computational model capture observed closing used it explore critical design features....
Atomic force microscopy images for [bmim][Tf2N] films deposited at ambient conditions by drop-casting show a population of terraced islands mesoscopic area (1–100 μ2) and ∼50 nm height. The regularity terraces steps, stiff mechanical properties fragile fracture mode all suggest that the are solid-like, even though bulk is liquid temperature experiment. Molecular dynamics simulations homogeneous film 4 thick on silica also display marked layering in proximity to periodicity closely matching...
The shoot apical meristem (SAM) gives rise to all aerial plant organs. Cell walls are thought play a central role in this process, translating molecular regulation into dynamic changes growth rate and direction, although their precise morphogenesis during organ formation is poorly understood. Here, we investigated the of xyloglucans (XyGs), major, yet functionally characterized, wall component SAM Arabidopsis (Arabidopsis thaliana). Using immunolabeling, biochemical analysis, genetic...
We report on the production of cluster-assembled nanostructured titanium oxide (ns-TiOx) surfaces with tailored wettability properties. The contact angle ns-TiOx changes in a controllable and reproducible way by changing morphology through roughness and/or annealing films air at different temperatures. With increasing temperature, reversible switching from hydrophobic to hydrophilic behavior can be obtained, due removal organic contaminants increase surface hydroxyl groups, while control...
Great emphasis is placed on the development of integrated approaches for synthesis and characterization ad hoc nanostructured platforms, to be used as templates with controlled morphology chemical properties investigation specific phenomena great relevance technological applications in interdisciplinary fields such biotechnology, medicine advanced materials. Here we discuss crucial role advantages thin film deposition strategies based cluster-assembling from supersonic cluster beams. We...
We report on the modification of electric properties imidazolium-based [BMIM][NTf2] ionic liquid upon surface confinement in sub-monolayer regime. Solid-like insulating nanostructures spontaneously form a variety substrates, at odds with and conductive nature same substances bulk phase. A systematic spatially resolved investigation by atomic force microscopy morphological, mechanical, electrical showed that this substance rearranges into lamellar high degree vertical order enhanced...
Highlights•VAMP7 is important for mechano-adaptation to the rigidity of cellular environment•Substrate regulates VAMP7 exocytosis•LRRK1 and VARP compete binding•LRRK1 generate a tug-of-war transport VAMP7SummaryThe cell environment can vary tremendously between tissues in pathological conditions. How this property may affect intracellular membrane dynamics still largely unknown. Here, using atomic force microscopy, we show that cells deficient secretory lysosome v-SNARE are impaired...
We investigate the structure of [bmim][Tf(2)N]/silica interface by simulating indentation a thin (4 nm) [bmim][Tf(2)N] film hard nanometric tip. The ionic liquid/silica is represented in atomistic detail, while tip modelled spherical mesoscopic particle interacting via an effective short-range potential. Plots normal force (F(z)) on as function its distance from silica surface highlight effect weak layering liquid structure, well progressive loss fluidity approaching surface. simulation...
Stem cell homeostasis in the shoot apical meristem involves a core regulatory feedback loop between signalling peptide CLAVATA3 (CLV3), produced stem cells, and transcription factor WUSCHEL, expressed underlying organising centre. clv3 mutant meristems display massive overgrowth, which is thought to be caused by overproliferation, although it unknown how uncontrolled divisions lead this altered morphology. Here, we reveal local buckling defects meristems, use analytical models show...
We present here the use of atomic force microscopy to indent plant tissues and recover its mechanical properties. Using two different microscopes in indentation mode, we show how measure an elastic modulus it evaluate cell wall In addition, also explain turgor pressure. The main advantages are that is non-invasive, relatively rapid (5~20 min), virtually any type living tissue superficially flat can be analyzed without need for treatment. resolution very good, depending on tip size number...
We present here the use of atomic force microscopy to indent plant tissues and recover its mechanical properties. Using two different microscopes in indentation mode, we show how measure an elastic modulus it evaluate cell wall In addition, also explain turgor pressure. The main advantages are that is non-invasive, relatively rapid (5~20 min), virtually any type living tissue superficially flat can be analyzed without need for treatment. resolution very good, depending on tip size number...
Abstract An in‐depth understanding of the mechanical properties dermis is indispensable to improve wound healing or slow‐down skin ageing. Despite crucial research issues for dermatological and cosmetic industries, very little known about behaviour at nanoscale level. This knowledge relevant not only human but also mouse since this animal model widely used in basic preclinical studies biology health. Here, we describe an original protocol that developed specifically measure using atomic...