A5014658371- Cellular Mechanics and Interactions
- Force Microscopy Techniques and Applications
- Ion-surface interactions and analysis
- Mass Spectrometry Techniques and Applications
- thermodynamics and calorimetric analyses
- Nanopore and Nanochannel Transport Studies
- Medical Imaging and Pathology Studies
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cardiac Fibrosis and Remodeling
- Melanoma and MAPK Pathways
- Wnt/β-catenin signaling in development and cancer
- Occupational exposure and asthma
- Nuclear Physics and Applications
- Analytical chemistry methods development
- Silicon Nanostructures and Photoluminescence
- Cell Adhesion Molecules Research
- Microtubule and mitosis dynamics
- Mechanical and Optical Resonators
Institute of Nuclear Physics, Polish Academy of Sciences
2016-2023
Institute of Nuclear Physics
2016
Polish Academy of Sciences
2016
We present a procedure that allows reliable determination of the elastic (Young’s) modulus soft samples, including living cells, by atomic force microscopy (AFM). The standardized nanomechanical AFM (SNAP) ensures precise adjustment optical lever system, prerequisite for all kinds spectroscopy methods, to obtain values independent instrument, laboratory and operator. Measurements hydrogel samples with well-defined using different AFMs revealed uncertainties in deflection sensitivity...
The identification of cancer-related changes in cells and tissues based on the measurements elastic properties using atomic force microscopy (AFM) seems to be approaching clinical application. Several limiting aspects have already been discussed; however, still, no data shown how specific AFM probe geometries are related biomechanical evaluation cancer cells. Here, we analyze compare nanomechanical results mechanically homogenous polyacrylamide gels heterogeneous bladder measured probes...
The multistep character of cancer progression makes it difficult to define a unique biomarker the disease. Interdisciplinary approaches, combining various complementary techniques, especially those operating at nanoscale level, potentially accelerate characterization cells or tissue properties. Here, we study relation between surface and biomechanical properties melanoma cells, measured by mass spectrometry (ToF-SIMS) atomic force microscopy (AFM). In total, seven cell lines have been...
Cardiac fibroblasts are able to sense the rigidity of their environment. The present study examines whether stiffness substrate in cardiac fibroblast culture can influence release interleukin-6 (IL-6), interleukin-11 (IL-11) and soluble receptor IL-6 (sIL-6R). It also roles integrin α2β1 activation intracellular signalling these processes. were cultured on polyacrylamide gels grafted collagen, with an elasticity E = 2.23 ± 0.8 kPa (soft gel) 8.28 1.06 (stiff gel, measured by Atomic Force...
The presented research aims to verify whether physicochemical properties of lung fibroblasts, modified by substrate stiffness, can be used discriminate between normal and fibrotic cells from idiopathic pulmonary fibrosis (IPF). impact polydimethylsiloxane (PDMS) stiffness on the (LL24) IPF-derived fibroblasts (LL97A) was examined in detail. growth elasticity were assessed using fluorescence microscopy atomic force working spectroscopy mode, respectively. number as well their shape...
Various techniques have been already reported to differentiate between normal (non-malignant) and cancerous cells based on their physico-chemical properties. This is relatively simple when studied originate from distant stages of cancer progression. Here, studies chemical properties two closely related human melanoma cell lines are presented: WM115 were taken the vertical growth phase while WM266-4 skin metastatic site same patient. Their by techniques, namely time-of-flight secondary ion...
The spread of tumor cells and the formation distant metastasis remain main causes mortality in cancer patients. However, mechanisms governing release from micro-environmental constraints unclear. E-cadherin negatively controls invasion epithelial by maintaining cell–cell contacts. Furthermore, inactivation triggers vitro. role is complex, as metastasizing maintain expression, which appears to have a positive survival cells. In this report, we present novel mechanism delineating how function...
Data included in this article are associated with the research entitled 'Protocol of single cells preparation for time-of-flight secondary ion mass spectrometry' (Bobrowska et al., 2016 press) [1]. This data file contains topography images melanoma recorded using atomic force microscopy (AFM). Single cultured on glass surface were subjected to proposed sample protocol applied prepare biological samples spectrometry (ToF SIMS) measurements. AFM collected step-by-step cell, after each step...