A5000498952- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Plant biochemistry and biosynthesis
- Plant Stress Responses and Tolerance
- Genetic Mapping and Diversity in Plants and Animals
- Plant nutrient uptake and metabolism
- Lipid metabolism and biosynthesis
- Metabolomics and Mass Spectrometry Studies
- Plant tissue culture and regeneration
- Plant responses to water stress
- Mitochondrial Function and Pathology
- GABA and Rice Research
- Postharvest Quality and Shelf Life Management
- Plant Virus Research Studies
- Plant Pathogenic Bacteria Studies
- Microbial Metabolic Engineering and Bioproduction
- Plant Reproductive Biology
- Genetics and Plant Breeding
- Light effects on plants
- Phytochemicals and Antioxidant Activities
- Berry genetics and cultivation research
- Plant Parasitism and Resistance
- Plant-Microbe Interactions and Immunity
- Algal biology and biofuel production
Center of Plant Systems Biology and Biotechnology
2018-2025
Max Planck Institute of Molecular Plant Physiology
2016-2025
Saskatchewan Research Council (Canada)
2021
National Research Council Canada
2021
Plovdiv University
2020
Max Planck Institute for Plant Breeding Research
2019
Max Planck Society
2013-2018
Hebron University
2009-2013
Hebrew University of Jerusalem
2013
Solanum pennellii is a wild tomato species endemic to Andean regions in South America, where it has evolved thrive arid habitats. Because of its extreme stress tolerance and unusual morphology, an important donor germplasm for the cultivated lycopersicum. Introgression lines (ILs) which large genomic S. lycopersicum are replaced with corresponding segments from can show remarkably superior agronomic performance. Here we describe high-quality genome assembly parents IL population. By...
Abstract Phenylpropanoids comprise an important class of plant secondary metabolites. A number transcription factors have been used to upregulate-specific branches phenylpropanoid metabolism, but by far the most effective has fruit-specific expression AtMYB12 in tomato, which resulted as much 10% fruit dry weight accumulating flavonols and hydroxycinnamates. We show that not only increases demand flavonoid biosynthesis also supply carbon from primary energy reducing power, may fuel shikimate...
Updates in nanopore technology have made it possible to obtain gigabases of sequence data. Prior this, sequencing was mainly used analyze microbial samples. Here, we describe the generation a comprehensive data set with median read length 11,979 bp for self-compatible accession wild tomato species Solanum pennellii We assembly its genome contig N50 2.5 MB. The pipeline comprised initial correction Canu and SMARTdenovo. resulting raw nanopore-based de novo is structurally highly similar that...
Abstract A large-scale metabolic quantitative trait loci (mQTL) analysis was performed on the well-characterized Solanum pennellii introgression lines to investigate genomic regions associated with secondary metabolism in tomato fruit pericarp. In total, 679 mQTLs were detected across 76 lines. Heritability analyses revealed that of less affected by environment than primary metabolism. Network allowed us assess interconnectivity and as well compare contrast their respective associations...
Abstract Wild teas are valuable genetic resources for studying domestication and breeding. Here we report the assembly of a high-quality chromosome-scale reference genome an ancient tea tree. The further RNA sequencing 217 diverse accessions clarifies pedigree cultivars reveals key contributors in breeding Chinese tea. Candidate genes associated with flavonoid biosynthesis identified by genome-wide association study. Specifically, allelic function CsANR , CsF3’5’H CsMYB5 is verified...
Reactive oxygen species (ROS)-dependent signaling pathways from chloroplasts and mitochondria merge at the nuclear protein RADICAL-INDUCED CELL DEATH1 (RCD1). RCD1 interacts in vivo suppresses activity of transcription factors ANAC013 ANAC017, which mediate a ROS-related retrograde signal originating mitochondrial complex III. Inactivation leads to increased expression dysfunction stimulon (MDS) genes regulated by ANAC017. Accumulating MDS gene products, including alternative oxidases...
Abstract Germination and early seedling establishment are developmental stages in which plants face limited nutrient supply as their photosynthesis mechanism is not yet active. For this reason, the plant must mobilize reserves provided by mother order to facilitate growth. Autophagy a catabolic process enabling bulk degradation of cellular constituents vacuole. The autophagy conserved among eukaryotes, homologs many autophagy-related (ATG) genes have been found Arabidopsis thaliana. T-DNA...
Deciphering the influence of genetics on primary metabolism in plants will provide insights useful for genetic improvement and enhance our fundamental understanding plant growth development. Although maize (Zea mays) is a major crop food feed worldwide, architecture its largely unknown. Here, we use high-density linkage mapping to dissect large-scale metabolic traits measured three different tissues (leaf at seedling stage, leaf reproductive kernel 15 d after pollination [DAP]) recombinant...
The maturation and development of tomato fruit has received much attention due both to the complexity intricacy changes which occur during this process importance these fruits as a component human diet. Whilst great advances have been made in understanding molecular genetic aspects development, our knowledge concerning metabolic shifts underpinning remains largely confined primary metabolism. Conversely, majority metabolites considered health benefits are secondary or specialized...
The genes coding for the core metabolic enzymes of photorespiratory pathway that allows plants with C3-type photosynthesis to survive in an oxygen-rich atmosphere, have been largely discovered genetic screens aimed isolate mutants are unviable under ambient air. As exception, glycolate oxidase (GOX) a phenotype not described yet C3 species. Using Arabidopsis (Arabidopsis thaliana) lacking peroxisomal CATALASE2 (cat2-2) display stunted growth and cell death lesions air, we isolated...
The plant tricarboxylic acid (TCA) cycle provides essential precursors for respiration, amino biosynthesis, and general nitrogen metabolism; moreover, it is closely involved in biotic stress responses cellular redox homeostasis. To further understand the vivo function of TCA enzymes, we combined affinity purification with proteomics to generate a comprehensive extra-pathway protein-protein interaction network cycle. We identified 125 interactions Arabidopsis (Arabidopsis thaliana) mostly...
Abstract Metabolomics has grown into one of the major approaches for systems biology studies, in part driven by developments mass spectrometry (MS), providing high sensitivity and coverage metabolome at throughput. Untargeted metabolomics allows investigation metabolic phenotypes involving several hundreds to thousands metabolites. In this approach, all signals a chromatogram are processed an unbiased way, allowing deeper phenotypes, but also resulting significantly more complex data...