Katherine N. Maloney
A5029565903- Microbial Natural Products and Biosynthesis
- Metabolomics and Mass Spectrometry Studies
- Marine Sponges and Natural Products
- Computational Drug Discovery Methods
- Cocoa and Sweet Potato Agronomy
- Genomics and Phylogenetic Studies
- Chemical synthesis and alkaloids
- Phytoplasmas and Hemiptera pathogens
- Enzyme Production and Characterization
- Research Data Management Practices
- Natural product bioactivities and synthesis
- Plant Pathogenic Bacteria Studies
- Advanced Chemical Sensor Technologies
- Microbial Metabolism and Applications
- Fungal Biology and Applications
- Bioactive Compounds and Antitumor Agents
- Scientific Computing and Data Management
- Proteins in Food Systems
- Ubiquitin and proteasome pathways
- Analytical Chemistry and Chromatography
- Ion Channels and Receptors
- Acute Lymphoblastic Leukemia research
- Polysaccharides Composition and Applications
- Biochemical and Structural Characterization
- Infectious Diseases and Mycology
Point Loma Nazarene University
2015-2025
Novozymes (United States)
2023
University of California, San Diego
2009-2020
Harvey Mudd College
2010-2020
University of Montana
2020
Scripps Institution of Oceanography
2009-2011
Dana-Farber Cancer Institute
2008
Harvard University
2006-2008
Genomics and metabolomics are widely used to explore specialized metabolite diversity. The Paired Omics Data Platform is a community initiative systematically document links between metabolome (meta)genome data, aiding identification of natural product biosynthetic origins structures.
Many transient receptor potential (TRP) channels are activated or blocked by various compounds found in plants; two prominent examples include the activation of TRPV1 capsaicin and TRPM8 menthol. We sought to identify additional plant that active on other types TRP channels. screened a library extracts from 50 Chinese herbal plants using calcium-imaging assay find TRPV3 TRPV4 An extract Andrographis paniculata potently The was fractionated further, compound identified as bisandrographolide A...
Bacteria of the genus Frankia are mycelium-forming actinomycetes that found as nitrogen-fixing facultative symbionts actinorhizal plants. Although soil-dwelling well-known producers bioactive compounds, has largely gone uninvestigated for this potential. Bioinformatic analysis genome sequences strains ACN14a, CcI3, and EAN1pec revealed an unexpected number secondary metabolic biosynthesis gene clusters. Our led to identification at least 65 biosynthetic clusters, vast majority which appear...
Chemical examination of the secondary metabolites a marine Saccharomonospora sp., isolated from sediments collected at mouth La Jolla Submarine Canyon, yielded unprecedented alkaloid lodopyridone (1). The low proton-to-carbon ratio 1 precluded structure elucidation by NMR spectroscopic methods, thus was defined X-ray crystallography. Lodopyridone is cytotoxic to HCT-116 human colon cancer cells with IC(50) = 3.6 microM.
Inhibitors of quinone reductase-2 (NQO2; QR-2) can have antimalarial activity and antitumor activities or function as chemoprevention agents by preventing the metabolic activation toxic quinones such menadione. To expedite search for new natural product inhibitors QR-2, we developed a screening assay based on ultrafiltration liquid chromatography−mass spectrometry that is compatible with complex samples bacterial botanical extracts. Human QR-2 was prepared recombinantly, known inhibitor,...
Huanglongbing (HLB) is a destructive citrus disease that lethal to all commercial plants, making it the most serious and one of plant diseases. Because severity HLB paucity effective control measures, we structured this study encompass entirety microbiome chemistries associated with microbial community. We describe spatial niche diversity bacteria fungi roots, stems, leaves using traditional culturing integrated culture-independent methods. Using culturable sector microbiome, created...
ABSTRACT Cellulose is the most abundant polymer on earth and plays a key role in carbon cycle, agriculture, human health. Many anaerobic cellulose-degrading bacteria produce uncharacterized yellow-orange, cellulose-binding pigments known as yellow affinity substances (here referred to flavoaffinins) that are associated with efficient cellulose degradation. Here, we isolate structurally characterize flavoaffinins from Clostridium ( Hungateiclostridium ) thermocellum , workhorse for industrial...
Phaeosphaeride A, a nitrogen-containing bicyclic compound produced by an endophytic fungus, inhibits signaling the transcription factor STAT3.
Natural products and their derivatives continue to be wellsprings of nascent therapeutic potential. However, many laboratories have limited resources for biological evaluation, leaving previously isolated or synthesized compounds largely completely untested. To address this issue, the Canvass library natural was assembled, in collaboration with academic industry researchers, quantitative high-throughput screening (qHTS) across a diverse set cell-based biochemical assays. Characterization...
Bioassay-guided fractionation of Physocarpus capitatus yielded two new cucurbitacins (3 and 4) along with the known cucurbitacin F (1) dihydrocucurbitacin (2). Preliminary mechanism action studies indicate that cause actin aggregates inhibit cell division.
Antartin (1), a new zizaane-type sesquiterpene, was isolated from Streptomyces sp. SCO736. The chemical structure of 1 assigned the interpretation 1D and 2D NMR in addition to mass spectrometric data. relative stereochemistry determined by analysis NOE data, while absolute decided based on comparison experimental calculated electronic circular dichroism (ECD) spectra. (1) showed cytotoxicity against A549, H1299, U87 cancer cell lines causing cycle arrest at G1 phase.
Abstract Access to web-based platforms has enabled scientists perform research remotely. A critical aspect of mass spectrometry data analysis is the inspection, analysis, and visualization raw validate quality confirm statistical observations. We developed GNPS Dashboard, a tool, facilitate synchronous collaborative visualization, private public
Sarcophyton glaucum is one of the most abundant and chemically studied soft corals with over 100 natural products reported in literature, primarily cembrane diterpenoids. Yet, wide variation chemistry observed from S. past 50 years has led to its reputation as a capricious producer bioactive metabolites. Recent molecular phylogenetic analysis revealed that not single species but complex at least seven genetically distinct distinguishable using traditional taxonomic criteria. We hypothesized...
Abstract Gas chromatography-mass spectrometry (GC-MS) represents an analytical technique with significant practical societal impact. Spectral deconvolution is essential step for interpreting GC-MS data. No public repositories that also enable repository-scale analysis exist, in part because requires user input. We therefore engineered a scalable machine learning workflow the Global Natural Product Social Molecular Networking (GNPS) platform to mass community store, process, share, annotate,...
Pierce's disease of grapevine and citrus huanglongbing are caused by the bacterial pathogens Xylella fastidiosa Candidatus Liberibacter asiaticus (CLas), respectively. Both reside within plant vascular system, occluding water nutrient transport, leading to a decrease in productivity fruit marketability ultimately death their hosts. Field observations apparently healthy plants disease-affected vineyards groves led hypothesis that natural products from endophytes may inhibit these pathogens....
ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionNEXTORIGINAL ARTICLEThis notice is a correctionCorrection to Screening Natural Products for Inhibitors of Quinone Reductase-2 Using Ultrafiltration LC–MSYongsoo Choi, Katherine Jermihov, Sang-Jip Nam, Megan Sturdy, Maloney, Xi Qiu, Lucas R. Chadwick, Matthew Main, Shao-Nong Chen, Andrew D. Mesecar, Norman Farnsworth, Guido F. Pauli, William Fenical, John M. Pezzuto, and Richard B. van Breemen*Cite this: Anal. Chem. 2011, 83, 11,...