Sneha Couvillion
A5023732378- Seismic Waves and Analysis
- Earthquake Detection and Analysis
- Methane Hydrates and Related Phenomena
- Ionosphere and magnetosphere dynamics
- Geophysics and Gravity Measurements
- GNSS positioning and interference
- Seismology and Earthquake Studies
- Astrophysics and Cosmic Phenomena
- Microbial Community Ecology and Physiology
- Metabolomics and Mass Spectrometry Studies
- Gut microbiota and health
- Soil Carbon and Nitrogen Dynamics
- Fungal Infections and Studies
- Marine Sponges and Natural Products
- Genomics and Phylogenetic Studies
- Advanced Proteomics Techniques and Applications
- Agriculture, Soil, Plant Science
- Antifungal resistance and susceptibility
- Probiotics and Fermented Foods
- Mass Spectrometry Techniques and Applications
- Extracellular vesicles in disease
- Microbial Natural Products and Biosynthesis
- Studies on Chitinases and Chitosanases
- Mycorrhizal Fungi and Plant Interactions
- Analytical Chemistry and Chromatography
Pacific Northwest National Laboratory
2018-2025
Battelle
2024
Environmental Molecular Sciences Laboratory
2019
Richland College
2018
Glioblastoma (GBM) is the most aggressive nervous system cancer. Understanding its molecular pathogenesis crucial to improving diagnosis and treatment. Integrated analysis of genomic, proteomic, post-translational modification metabolomic data on 99 treatment-naive GBMs provides insights GBM biology. We identify key phosphorylation events (e.g., phosphorylated PTPN11 PLCG1) as potential switches mediating oncogenic pathway activation, well targets for EGFR-, TP53-, RB1-altered tumors. Immune...
Despite advances in sequencing, lack of standardization makes comparisons across studies challenging and hampers insights into the structure function microbial communities multiple habitats on a planetary scale. Here we present multi-omics analysis diverse set 880 community samples collected for Earth Microbiome Project. We include amplicon (16S, 18S, ITS) shotgun metagenomic sequence data, untargeted metabolomics data (liquid chromatography-tandem mass spectrometry gas chromatography...
Candida auris was first described in Japan 2009 and has now been the cause of significant outbreaks across globe. The high number isolates that are resistant to one or more antifungals, as well mortality rates from patients with bloodstream infections, attracted attention medical mycology, infectious disease, public health communities this pathogenic fungus. In current work, we performed a broad multi-omics approach on two clinical isolated New York, most affected area United States found...
Candida auris is a recently described multidrug-resistant pathogenic fungus that increasingly responsible for health care-associated outbreaks across the world. Bloodstream infections of this cause death in up to 70% cases. Aggravating scenario, disease-promoting mechanisms C. are poorly understood. Fungi release extracellular vesicles (EVs) carry broad range molecules, including proteins, lipids, carbohydrates, pigments, and RNA, many which virulence factors. Here, we carried out...
Generating top-down tandem mass spectra (MS/MS) from complex mixtures of proteoforms benefits improvements in fractionation, separation, fragmentation, and analysis. The algorithms to match MS/MS sequences have undergone a parallel evolution, with both spectral alignment match-counting approaches producing high-quality proteoform-spectrum matches (PrSMs). This study assesses state-of-the-art for identification (ProSight PD, TopPIC, MSPathFinderT, pTop) their yield PrSMs while controlling...
Although genomic anomalies in glioblastoma (GBM) have been well studied for over a decade, its 5-year survival rate remains lower than 5%. We seek to expand the molecular landscape of high-grade glioma, composed IDH-wildtype GBM and IDH-mutant grade 4 astrocytoma, by integrating proteomic, metabolomic, lipidomic, post-translational modifications (PTMs) with transcriptomic measurements uncover multi-scale regulatory interactions governing tumor development evolution. Applying 14 proteogenomic...
Histoplasma capsulatum is a dimorphic fungus that most frequently causes pneumonia, but can also disseminate and proliferate in diverse tissues. has complex secretion system mediates the release of macromolecule-degrading enzymes virulence factors. The formation extracellular vesicles (EVs) are an important mechanism for non-conventional both ascomycetes basidiomycetes. EVs contain proteins associated with immunologically active. Despite growing knowledge from H. other pathogenic fungi,...
Maternal obesity puts the offspring at high risk of developing and cardio-metabolic diseases in adulthood. Here, we utilized a mouse model maternal high-fat diet (HFD)-induced that recapitulates metabolic perturbations seen humans. We show increased adiposity HFD-fed mothers (Off-HFD) when compared to regular diet-fed (Off-RD). have previously reported significant immune bone marrow newly-weaned Off-HFD. hypothesized lipid metabolism is altered Off-HFD vs. Off-RD. To test this hypothesis,...
Sponges are ancient sessile metazoans, which form with their associated microbial symbionts a complex functional unit called holobiont. rich source of chemical diversity; however, there is limited knowledge holobiont members produce certain metabolites and how they may contribute to interactions. To address this issue, we applied non-targeted liquid chromatography tandem mass spectrometry (LC-MS/MS) gas (GC-MS) either whole sponge tissue or fractionated cells from six different, co-occurring...
Soil microorganisms provide key ecological functions that often rely on metabolic interactions between individual populations of the soil microbiome. To better understand these and community processes, we used chitin, a major carbon nitrogen source in soil, as test substrate to investigate microbial during its decomposition. Chitin was applied model consortium developed, "model consortium-2" (MSC-2), consisting eight members diverse phyla including both chitin degraders nondegraders. A...
Abstract Background Microbiomes contribute to multiple ecosystem services by transforming organic matter in the soil. Extreme shifts environment, such as drying-rewetting cycles during drought, can impact microbial metabolism of altering physiology and function. These physiological responses are mediated part lipids that responsible for regulating interactions between cells environment. Despite this critical role response stress, little is known about metabolites soil or how they influence...
ABSTRACT Soil moisture and porosity regulate microbial metabolism by influencing factors, such as system chemistry, substrate availability, soil connectivity. However, accurately representing the environment establishing a tractable community that limits confounding variables is difficult. Here, we use reduced-complexity consortium grown in glass bead porous media amended with chitin to test effects of structural matrix on phenotypes. Leveraging metagenomes, metatranscriptomes,...
Two factors that are well-known to influence soil microbiomes the depth of as well level moisture. Previous works have demonstrated climate change will increase incidence drought in soils, but it is unknown how fluctuations moisture availability affect microbiome composition and functioning down profile. Here, we investigated wheatgrass rhizosphere a single common field setting under four different levels irrigation (100%, 75%, 50%, 25%) three depths (0-5 cm, 5-15 15-25 cm from surface). We...
Lipids play a fundamental role in fungal cell biology, being essential membrane components and major targets of antifungal drugs. A deeper knowledge lipid metabolism is key for developing new drugs better understanding pathogenesis. Here, we built comprehensive map the Histoplasma capsulatum metabolic pathway by incorporating proteomic lipidomic analyses. We performed genetic complementation overexpression H. genes Saccharomyces cerevisiae to validate reactions identified determine enzymes...
ABSTRACT Climate change is causing an increase in drought many soil ecosystems and a loss of organic carbon. Calcareous soils may partially mitigate these losses via carbon capture storage. Here, we aimed to determine how irrigation-supplied moisture perennial plants impact biotic abiotic properties that underpin deep chemistry unfertilized calcareous soil. Soil was sampled up 1 m depth from irrigated planted field treatments analyzed using suite omics chemical analyses. The microbial...
Soil moisture and porosity regulate microbial metabolism by influencing factors such as redox conditions, substrate availability, soil connectivity. However, the inherent biological, chemical, physical heterogeneity of complicates laboratory investigations into phenotypes that mediate community metabolism. This difficulty arises from challenges in accurately representing environment establishing a tractable limits confounding variables. To address these our investigation metabolism, we use...