A5004588687- Seismic Waves and Analysis
- Methane Hydrates and Related Phenomena
- Earthquake Detection and Analysis
- Ionosphere and magnetosphere dynamics
- Geophysics and Gravity Measurements
- Solar and Space Plasma Dynamics
- Astrophysics and Cosmic Phenomena
- Superconducting Materials and Applications
- Soil Moisture and Remote Sensing
- Microbial Community Ecology and Physiology
- Gut microbiota and health
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Soil Carbon and Nitrogen Dynamics
- Metabolomics and Mass Spectrometry Studies
- Clostridium difficile and Clostridium perfringens research
- Microbial bioremediation and biosurfactants
- Climate change and permafrost
- Inflammatory Bowel Disease
- Plant-Microbe Interactions and Immunity
- Polar Research and Ecology
- bioluminescence and chemiluminescence research
- Plant Virus Research Studies
- Mycorrhizal Fungi and Plant Interactions
- Bioinformatics and Genomic Networks
Pacific Northwest National Laboratory
2015-2024
Lawrence Berkeley National Laboratory
2008-2022
Mammoth Biosciences (United States)
2022
Environmental Molecular Sciences Laboratory
2022
Huazhong Agricultural University
2022
Oak Ridge National Laboratory
2009-2022
Stanford Synchrotron Radiation Lightsource
2022
Battelle
2016-2020
Joint BioEnergy Institute
2011-2018
Richland College
2018
Designing primers for PCR-based taxonomic surveys that amplify a broad range of phylotypes in varied community samples is difficult challenge, and the comparability data sets amplified with requires attention. Here, we examined performance modified 16S rRNA gene internal transcribed spacer (ITS) archaea/bacteria fungi, respectively, nonaquatic samples. We moved primer bar codes to 5' end, allowing different 3' pairings, such as 515f/926r pair, which amplifies variable regions 4 5 gene....
The biological effects and expected fate of the vast amount oil in Gulf Mexico from Deepwater Horizon blowout are unknown owing to depth magnitude this event. Here, we report that dispersed hydrocarbon plume stimulated deep-sea indigenous γ-Proteobacteria closely related known petroleum degraders. Hydrocarbon-degrading genes coincided with concentration various contaminants. Changes composition distance source incubation experiments environmental isolates demonstrated faster-than-expected...
Antibiotic administration is the standard treatment for bacterium Helicobacter pylori, main causative agent of peptic ulcer disease and gastric cancer. However, long-term consequences this on human indigenous microbiota are relatively unexplored. Here we studied short- effects clarithromycin metronidazole treatment, a commonly used therapy regimen against H. in throat lower intestine. The bacterial compositions samples collected over four-year period were monitored by analyzing 16S rRNA gene...
The Earth Microbiome Project (EMP) was launched in August 2010, with the ambitious aim of constructing a global catalogue uncultured microbial diversity this planet. primary vision Project, to process and functional potential from approximately 200,000 environmental samples, marks it as an undertaking so massive that at first considered be pure folly (as late 2012, Jonathan Eisen quoted Nature saying ‘Knight
Although much work has linked the human microbiome to specific phenotypes and lifestyle variables, data from different projects have been challenging integrate extent of microbial molecular diversity in stool remains unknown. Using standardized protocols Earth Microbiome Project sample contributions over 10,000 citizen-scientists, together with an open research network, we compare specimens primarily United States, Kingdom, Australia one another environmental samples. Our results show...
Clostridium difficile-associated disease (CDAD) is the major known cause of antibiotic-induced diarrhea and colitis, thought to result from persistent disruption commensal gut microbiota. Bacteriotherapy by way fecal transplantation can be used treat recurrent CDAD, which reestablish normal colonic microflora. However, limitations conventional microbiologic techniques have, until recently, precluded testing this idea. In study, we terminal-restriction fragment length polymorphism 16S rRNA...
We developed a protocol which yields purified bacterial DNA from the soil community. The bacteria were first dispersed and separated particles in presence of polyvinylpolypyrrolidone, removes humic acid contaminants by adsorption to this insoluble polymer. then collected centrifugation lysed using comprehensive designed maximize disruption various types present. Total was cell lysate remaining equilibrium density gradients. isolated essentially pure as determined UV spectral analysis, at...
Abstract The Deepwater Horizon oil spill in the Gulf of Mexico resulted a deep-sea hydrocarbon plume that caused shift indigenous microbial community composition with unknown ecological consequences. Early history, bloom uncultured, thus uncharacterized, members Oceanospirillales was previously detected, but their role disposition unknown. Here our aim to determine functional and other active using deep sequencing DNA RNA, as well single-cell genomics. Shotgun metagenomic metatranscriptomic...