A5032759284- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Inflammatory Bowel Disease
- Diet and metabolism studies
- Helicobacter pylori-related gastroenterology studies
- Gastrointestinal motility and disorders
- Microscopic Colitis
- Digestive system and related health
- Probiotics and Fermented Foods
- Nutritional Studies and Diet
- Immune Response and Inflammation
- Peroxisome Proliferator-Activated Receptors
- Clinical Nutrition and Gastroenterology
- Drug Transport and Resistance Mechanisms
- Eosinophilic Esophagitis
- Bacteriophages and microbial interactions
- Mycobacterium research and diagnosis
- Cytokine Signaling Pathways and Interactions
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Respiratory viral infections research
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Infant Nutrition and Health
- Immune Cell Function and Interaction
University of Pennsylvania
2016-2025
California University of Pennsylvania
2012-2025
Stony Brook University
2025
Hospital of the University of Pennsylvania
2012-2024
Philadelphia University
2018-2023
Pennsylvania Hospital
2017-2023
Franklin University
2022
Rosalind Franklin University of Medicine and Science
2022
West Virginia University
2019
Research Network (United States)
2018
Diet strongly affects human health, partly by modulating gut microbiome composition. We used diet inventories and 16S rDNA sequencing to characterize fecal samples from 98 individuals. Fecal communities clustered into enterotypes distinguished primarily levels of Bacteroides Prevotella. Enterotypes were associated with long-term diets, particularly protein animal fat (Bacteroides) versus carbohydrates (Prevotella). A controlled-feeding study 10 subjects showed that composition changed...
Abstract Motivation: The human microbiome plays an important role in disease and health. Identification of factors that affect the composition can provide insights into mechanism as well suggest ways to modulate for therapeutical purposes. Distance-based statistical tests have been applied test association with environmental or biological covariates. unweighted weighted UniFrac distances are most widely used distance measures. However, these two measures assign too much weight either rare...
Immense populations of viruses are present in the human gut and other body sites. Understanding role these (the “virome”) health disease requires a much deeper understanding their composition dynamics face environmental perturbation. Here, we investigate viromes from subjects on controlled feeding regimen. Longitudinal fecal samples were analyzed by metagenomic sequencing DNA virus-like particles (VLP) total microbial communities. Assembly 336 Mb VLP sequence yielded 7175 contigs, many...
Peroxisome proliferator-activated receptor γ (PPAR-γ), a member of the nuclear hormone superfamily originally shown to play critical role in adipocyte differentiation and glucose homeostasis, has recently been implicated as regulator cellular proliferation inflammatory responses. Colonic epithelial cells, which express high levels PPAR-γ protein, have ability produce cytokines that may bowel disease (IBD). We report here ligands dramatically attenuate cytokine gene expression colon cancer...
Although much work has linked the human microbiome to specific phenotypes and lifestyle variables, data from different projects have been challenging integrate extent of microbial molecular diversity in stool remains unknown. Using standardized protocols Earth Microbiome Project sample contributions over 10,000 citizen-scientists, together with an open research network, we compare specimens primarily United States, Kingdom, Australia one another environmental samples. Our results show...
Diet influences health as a source of nutrients and toxins, by shaping the composition resident microbial populations. Previous studies have begun to map out associations between diet bacteria viruses human gut microbiome. Here we investigate with fungal archaeal populations, taking advantage samples from 98 well-characterized individuals. was quantified using inventories scoring both long-term recent diet, archaea fungi were characterized deep sequencing marker genes in DNA purified stool....
We have identified a family of resistin-like molecules (RELMs) in rodents and humans. Resistin is hormone produced by fat cells. RELMα secreted protein that has restricted tissue distribution with highest levels adipose tissue. Another member, RELMβ, expressed only the gastrointestinal tract, particularly colon, both mouse human. RELMβ gene expression proliferative epithelial cells markedly increased tumors, suggesting role intestinal proliferation. RELMs share cysteine composition other...
Humans are colonized by immense populations of viruses, which metagenomic analysis shows mostly unique to each individual. To investigate the origin and evolution human gut virome, we analyzed viral community one adult individual over 2.5 y extremely deep sequencing (56 billion bases purified sequence from 24 longitudinal fecal samples). After assembly, 478 well-determined contigs could be identified, inferred correspond previously unstudied bacteriophage genomes. Fully 80% these types...
<h3>Objective</h3> The consumption of an agrarian diet is associated with a reduced risk for many diseases 'Westernised' lifestyle. Studies suggest that affects the gut microbiota, which subsequently influences metabolome, thereby connecting diet, microbiota and health. However, degree to composition controversial. Murine models studies comparing in humans residing versus Western societies influence large. To separate global environmental from dietary influences, we characterised host...
Intense interest centers on the role of human gut microbiome in health and disease, but optimal methods for analysis are still under development. Here we present a study surveying bacterial communities feces using 454/Roche pyrosequencing 16S rRNA gene tags. We analyzed fecal samples from 10 individuals compared storage, DNA purification sequence acquisition. To assess reproducibility, one cm apart single stool specimen each individual. analyze storage methods, 1) immediate freezing at...
In the present report, we show that human fecal microbiota contains promising and universal biomarkers for noninvasive evaluation of inflammatory bowel disease severity IFX treatment efficacy, emphasizing potential ability to mine gut as a modality stratify IBD patients apply personalized therapy optimal outcomes.
Inflammatory bowel disease (IBD) involves dysregulation of mucosal immunity in response to environmental factors such as the gut microbiota. The bacterial microbiota is often altered IBD, but connection not fully clarified and fungi have recently been suggested play a role well. In this study, we compared microbes from all 3 domains life-bacteria, archaea, eukaryota-in pediatric patients with IBD healthy controls.A stool sample was collected (n = 32) or control subjects 90), bacterial,...