A5081824427- Gut microbiota and health
- Advanced Condensed Matter Physics
- Inflammatory Bowel Disease
- Advanced biosensing and bioanalysis techniques
- Multiferroics and related materials
- Diet and metabolism studies
- Luminescence and Fluorescent Materials
- IL-33, ST2, and ILC Pathways
- Eosinophilic Esophagitis
- Microscopic Colitis
- Molecular Sensors and Ion Detection
- Escherichia coli research studies
- Genetic and Kidney Cyst Diseases
- Nuclear materials and radiation effects
- Microbial Community Ecology and Physiology
- Magnetic and transport properties of perovskites and related materials
- Organic Light-Emitting Diodes Research
- Dietary Effects on Health
- Infant Nutrition and Health
- Probiotics and Fermented Foods
- Electrochemical Analysis and Applications
- Conducting polymers and applications
- Genomics and Phylogenetic Studies
- Child Nutrition and Water Access
- Viral gastroenteritis research and epidemiology
Washington University in St. Louis
2013-2024
St. Louis VA Medical Center
2021-2022
Princeton University
2006-2008
Missouri State University
2001-2005
The normal range of physiological and metabolic phenotypes has been shaped by coevolution with microbial symbionts.
Metagenomic analyses of gnotobiotic mice and monozygotic twins reveal the effects eating a popular fermented milk product on their microbiomes.
Artificial human gut microbial communities implanted into germ-free mice provide insights how species-level responses to changes in diet give rise community-level structural and functional reconfiguration types of bacteria prioritize use available nutrients vivo.
The colonic epithelium can undergo multiple rounds of damage and repair, often in response to excessive inflammation. responsive stem cell that mediates this process is unclear, part because a lack vitro models recapitulate key epithelial changes occur vivo during repair. Here, we identify Hopx+ colitis-associated regenerative (CARSC) population functionally contributes mucosal repair mouse colitis. CARSCs, enriched for fetal-like markers, transiently arose from hypertrophic crypts known...
Alterations of the mycobiota composition associated with Crohn's disease (CD) are challenging to link defining elements pathophysiology, such as poor injury repair. Using culture-dependent and -independent methods, we discovered that Debaryomyces hansenii preferentially localized was abundant within incompletely healed intestinal wounds mice inflamed mucosal tissues CD human subjects. D. cultures from injured impaired colonic healing when introduced into conventionally raised or gnotobiotic...
Studies in mice indicate that the gut microbiota promotes energy harvest and storage from components of diet when these are plentiful. Here we examine how shapes host metabolic physiologic adaptations to periods nutrient deprivation. Germ-free (GF) who had received a transplant conventionally raised donors were compared fed fasted states by using functional genomic, biochemical, assays. A 24-h fast produces marked change microbial ecology. Short-chain fatty acids generated fermentation...
Anaerobic digesters rely on the diversity and distribution of parallel metabolic pathways mediated by complex syntrophic microbial communities to maintain robust optimal performance. Using mesophilic swine waste digesters, we experimented with increased ammonia loading induce a shift from aceticlastic methanogenesis an alternative acetate-consuming pathway acetate oxidation. In comparison control observed shifts in bacterial 16S rRNA gene content functional repertoires over course digesters'...
Primary cilia are specialized cell-surface organelles that mediate sensory perception and, in contrast to motile and flagella, thought lack motility function. Here, we show primary human mouse pancreatic islets exhibit movement is required for glucose-dependent insulin secretion. Islet contain motor proteins conserved from those found classic cilia, their three-dimensional motion dynein-driven dependent on adenosine 5′-triphosphate glucose metabolism. Inhibition of blocks beta cell calcium...
SERPINE1/PAI-1 is elevated in the colon tissue of most difficult-to-treat patients with IBD and leads to worsening experimental colitis.
The electrogenerated chemilumescence (ECL) of F(Ir)pic (bis(3,5-difluoro-2-(2-pyridyl)phenyl-(2-carboxypyridyl))iridium III) and (btp)2Ir(acac) (bis[2,(2'-benzothienyl)-pyridinato-N,C3'](acetlyacetonate)Ir(III) has been investigated in acetonitrile (MeCN), mixed MeCN/H2O (50:50, v/v), aqueous solutions using the oxidative reductive coreactant tri-n-propylamine. ECL was also studied presence nonionic surfactant Triton X-100 (poly-(ethylene glycol) tert-octylphenyl ether). is a blue emitter...
The effects of nonionic surfactant chain length on the properties tris(2,2'-bipyridyl)ruthenium(II) (Ru(bpy)3(2+) where bpy = 2,2'-bipyridine) electrochemiluminescence (ECL) have been investigated. electrochemistry, photophysics, and ECL Ru(bpy)3(2+) in presence a series surfactants are reported (Triton X-100, 114, 165, 405, 305, 705-70). These differ number poly(ethylene oxide) units incorporated into molecule. anodic oxidation produces tri-n-propylamine (TPrA) aqueous solution. Increases...
The effects of metal ions on the electrochemiluminescence (ECL) properties (bpy)2Ru(AZA-bpy) (bpy = 2,2'-bipyridine; AZA-bpy 4-(N-aza-18-crown-6-methyl-2,2'-bipyridine) have been investigated. electrochemistry, photophysics and ECL Ru(bpy)3(2+) in presence Pb2+, Hg2+, Cu2+, K+ are reported. anodic oxidation produces tri-n-propylamine (TPrA) 50:50 (v/v) CH3CN:H2O solution. Increases efficiency (photons generated per redox event) up to 20-fold that depend both concentration nature ion...
The effects of the nonionic surfactant Triton X-100 (poly(ethylene glycol) tert-octylphenyl ether) on properties tris(2-phenylpyridine)iridium(III) (Ir(ppy)3, where ppy = 2-phenylpyridine, electrochemiluminescence (ECL) have been investigated. Anodic oxidation Ir(ppy)3 produces ECL in presence tri-n-propylamine (TPrA) aqueous solution. Increases efficiency (≥10-fold) and TPrA current (≥2.0-fold) observed media. data support adsorption electrode surface, thus facilitating leading to higher...
The crystal structures of the A(2)B(2)O(7-x) niobium based pyrochlores Y(2)(Nb(0.86)Y(0.14))(2)O(6.91), CaYNb(2)O(7), and Y(2)NbTiO(7) are reported, determined by means powder neutron diffraction. These compounds represent first observation B-site displacements in pyrochlore structure: ions found to be displaced from ideal positions, creating electric dipoles. orientations these dipoles fully analogous magnetic moments Ising spin magnetically frustrated pyrochlores. Diffuse scattering...
Objective Fibrosis is a common feature of Crohn’s disease (CD) which can involve the mesenteric fat. However, molecular signature this process remains unclear. Our goal was to define transcriptional fibrosis in CD subjects and model mice improve our understanding pathogenesis. Design We performed histological analysis samples. modelled CD-like phenotype by performing repeated colonic biopsies analysed histology, type I collagen-targeted positron emission tomography (PET) global gene...
The electrochemiluminescence (ECL) of tris(8-hydroxyquinoline-5-sulfonic acid)aluminum(III) in aqueous solution is reported. ECL generated by complexing aluminum ions with the chelating agent 8-hydroxyquinoline-5-sulfonic acid (HQS) to form Al(HQS)3, followed oxidation presence tri-n-propylamine (TPrA). intensity peaks a potential corresponding both TPrA and emission spectrum (lambda(max) = 499 nm) matches photoluminescence spectrum, indicating that from Al(HQS)3* excited state. efficiencies...