A5051667337- Pancreatic function and diabetes
- Genetic and Kidney Cyst Diseases
- Renal and related cancers
- Genetic Syndromes and Imprinting
- Pancreatic and Hepatic Oncology Research
- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Micro and Nano Robotics
- Pluripotent Stem Cells Research
- Genomics and Chromatin Dynamics
- Legume Nitrogen Fixing Symbiosis
- Single-cell and spatial transcriptomics
- Microtubule and mitosis dynamics
- Pancreatitis Pathology and Treatment
Washington University in St. Louis
2021-2024
Primary cilia are specialized cell-surface organelles that mediate sensory perception and, in contrast to motile and flagella, thought lack motility function. Here, we show primary human mouse pancreatic islets exhibit movement is required for glucose-dependent insulin secretion. Islet contain motor proteins conserved from those found classic cilia, their three-dimensional motion dynein-driven dependent on adenosine 5′-triphosphate glucose metabolism. Inhibition of blocks beta cell calcium...
Abstract Mutations reducing the function of MYT1L, a neuron-specific transcription factor, are associated with syndromic neurodevelopmental disorder. MYT1L is used as pro-neural factor in fibroblast-to-neuron transdifferentiation and hypothesized to influence neuronal specification maturation, but it not clear which neuron types most impacted by loss. In this study, we profile 412,132 nuclei from forebrains wild-type MYT1L-deficient mice at three developmental stages: E14 peak neurogenesis,...
Indole-3-acetic acid (IAA), a form of the plant hormone auxin, is used by many plant-associated bacteria as cue to sense environment. Previously, we showed that IAA can promote disease in interactions between pathogen Pseudomonas syringae strain Pto DC000 and one its hosts, Arabidopsis thaliana .
Primary cilia are slender cell-surface organelles that project into the intercellular space. In pancreatic beta cells, primary coordinate a variety of cell responses including GPCR signaling, calcium influx, and insulin secretion, along with likely many underappreciated roles in islet development differentiation. To study function biology, direct visualization by microscopic methods is often necessary first step. Ciliary abundance, distribution, morphology heterogeneous among cells best...
Somatostatin is a paracrine modulator of insulin secretion and beta cell function with pleotropic effects on glucose homeostasis. The mechanism somatostatin-mediated communication between delta cells not well-understood, which we address in this study via the ciliary somatostatin receptor 3 (SSTR3). Primary cilia are membrane organelles that act as signaling hubs islets by virtue their subcellular location enrichment proteins such G-protein coupled receptors (GPCRs). We show SSTR3, GPCR,...
SUMMARY Pancreatic islet cells harbor primary cilia, small sensory organelles that detect environmental changes to regulate hormone secretion and intercellular communication. While the signaling capacity of cilia are well-appreciated, it is less recognized these also possess active motility, including in dense multicellular tissues such as pancreatic islet. In this manuscript, we use transgenic reporter mice an intravital imaging approach quantitate dynamics occurs live mouse islets. We...
ABSTRACT Primary cilia are specialized cell-surface organelles that mediate sensory perception and, in contrast to motile and flagella, thought lack motility function. Here we show primary pancreatic beta cells exhibit movement is required for glucose-dependent insulin secretion. Beta cell contain motor proteins conserved from those found classic cilia, their 3D motion dynein-driven dependent on ATP glucose metabolism. Inhibition of blocks calcium influx humans with type 2 diabetes have...