A5023029841- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Advanced Chemical Sensor Technologies
- Cardiovascular Syncope and Autonomic Disorders
- Mass Spectrometry Techniques and Applications
- Cardiac Arrhythmias and Treatments
- Botulinum Toxin and Related Neurological Disorders
- Neonatal Health and Biochemistry
- Chromatography in Natural Products
- Reproductive Biology and Fertility
- Gut microbiota and health
- Recycling and Waste Management Techniques
- Edible Oils Quality and Analysis
- Fatty Acid Research and Health
- Birth, Development, and Health
- Meat and Animal Product Quality
- Extraction and Separation Processes
- Bioactive Compounds and Antitumor Agents
- Gestational Diabetes Research and Management
- Neonatal Respiratory Health Research
- Horticultural and Viticultural Research
- Nosocomial Infections in ICU
- Cancer therapeutics and mechanisms
- Nutritional Studies and Diet
- Retinopathy of Prematurity Studies
Aristotle University of Thessaloniki
2016-2025
Telio (Norway)
2023
Centre for Research and Technology Hellas
2019
The paper reports the development of a multianalyte method and its application in metabolic profiling biological fluids. initial aim was quantification metabolites existing cell culture medium used in-vitro fertilization (IVF) other fluids related to embryo growth. Since most these analytes are polar primary hydrophilic interaction liquid chromatography system selected. analytical comprised Ultra-HPLC with detection on triple quadrupole mass spectrometer operating both positive negative...
Introduction: Acute lymphoblastic leukemia (ALL) is the most prevalent childhood malignancy. Despite high cure rates, several questions remain regarding predisposition, response to treatment, and prognosis of disease. The role intermediary metabolism in individualized mechanistic pathways disease unclear. We have hypothesized that children with any (sub)type ALL a distinct metabolomic fingerprint at diagnosis when compared: (i) control group; (ii) different ALL; (iii) end induction...
Preterm delivery (PTD) represents a major health problem that occurs in 1 10 births. The hypothesis of the present study was metabolic profile different biological fluids, obtained from pregnant women during second trimester gestation, could allow useful correlations with pregnancy outcome. Holistic and targeted metabolomics approaches were applied for complementary assessment content prospectively collected amniotic fluid (AF) paired maternal blood serum samples 35 who delivered preterm...
Pharmaceuticals have been classified as emerging contaminants in the aquatic ecosystem, mainly due to their increased use and improper disposal. A significant range of pharmaceutical compounds metabolites globally detected surface waters pose detrimental effects non-target organisms. Monitoring water pollution relies on analytical approaches for detection, however, such are limited by sensitivity limit coverage wide compounds. This lack realism risk assessment is bypassed with effect-based...
The aim of this pilot study is to evaluate sarcosine, uracil, and kynurenic acid in urine as potential biomarkers prostate cancer detection progression monitoring. Sarcosine, were measured samples 32 patients prior radical prostatectomy, 101 with increased prostate-specific antigen ultrasonographically-guided prostatic biopsy collected before after massage, 15 healthy volunteers (controls). results related histopathologic data, Gleason score, PSA (Prostate Specific Antigen). Metabolites...
A headspace-solid phase microextraction/gas chromatography-mass spectrometry (HS-SPME/GC-MS) method was developed herein for the analysis of virgin olive oil volatile metabolome. Optimisation SPME conditions performed by Design Experiments (DoE) and Response Surface Methodology (RSM) approaches factors, such as sample volume, stirring, extraction temperature time, desorption were examined to reach optimal microextraction conditions. The potential optimised then investigated its use in...
Metabolic phenotyping studies using mouse liver extracts as a model, performed on novel zwitterionic HILIC UHPLC column, which is based ethylene-bridged hybrid organic/inorganic particles bonded with sulfobetaine groups and packed into column hardware modified surface technology are reported. Initially the chromatographic performance was evaluated under different mobile phase conditions selected metabolite standards. Following optimization of for 88 hydrophilic metabolites both targeted...
Metabolomics is an interdisciplinary field with applications in many areas. Analytes include metabolites involved pathways such as glycolysis, amino acid metabolism, the Krebs cycle, etc. However, these biosynthetic are typically highly polar molecules, which represent a major challenge for chromatographic analysis. Whilst there have been significant efforts to address difficulties determination of comprehensive profiling metabolome remains problematic. The current review summarizes...
The effect of endogenous interferences serum in multi-targeted metabolite profiling HILIC-MS/MS analysis was investigated by studying different sample preparation procedures. A modified QuEChERS dispersive SPE protocol, a HybridSPE and combination liquid extraction with protein precipitation were compared to simple precipitation. Evaluation efficiency clean-up performed for all methods. sorbent materials tested found retain hydrophilic analytes together interferences, thus additional elution...
Alcoholic liver disease (ALD) as a consequence of ethanol chronic consumption could lead to hepatic cirrhosis that is linked high morbidity and mortality. Disease diagnosis still very challenging usually clear findings are obtained in the later stage ALD. The profound effect on metabolism can be depicted using metabolomics; thus, discovery novel biomarkers shed light initiation progression ALD, serving diagnostic purposes. In present study, Hydrophilic Interaction Liquid Chromatography...
Whey protein, a by-product of the cheese industry, can be putatively used as functional food due to its beneficial health properties. The main objective present study was assess in vivo effect sheep/goat whey protein on plasma amino acid profile and mammalian target rapamycin (mTOR), regulator skeletal myogenesis. A control group fed with standard commercial diet while experimental received plus for 28 days. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) conducted determine levels...