A5061761689- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Pharmacogenetics and Drug Metabolism
- Mass Spectrometry Techniques and Applications
- Advanced Chemical Sensor Technologies
- Drug Transport and Resistance Mechanisms
- Drug-Induced Hepatotoxicity and Protection
- Microfluidic and Capillary Electrophoresis Applications
- Gut microbiota and health
- Chromatography in Natural Products
- NMR spectroscopy and applications
- Advanced Proteomics Techniques and Applications
- Metabolism and Genetic Disorders
- Analytical chemistry methods development
- Neurobiology and Insect Physiology Research
- Analytical Methods in Pharmaceuticals
- Advanced NMR Techniques and Applications
- Advanced MRI Techniques and Applications
- Liver Disease Diagnosis and Treatment
- Diet and metabolism studies
- Alcohol Consumption and Health Effects
- Computational Drug Discovery Methods
- Molecular spectroscopy and chirality
- Traditional Chinese Medicine Analysis
- Inflammatory mediators and NSAID effects
Imperial College London
2016-2025
University of Liverpool
2006-2023
Imperial Valley College
2014-2023
Hammersmith Hospital
2023
AstraZeneca (United Kingdom)
2006-2020
Transnational Press London
2015-2020
Faculty (United Kingdom)
2020
Molecular Research Institute
2016
London Cancer
2016
Kensington Health
2015
We show here the identity of Alamar Blue as resazurin. The 'resazurin reduction test' has been used for about 50 years to monitor bacterial and yeast contamination milk, also assessing semen quality. Resazurin (blue nonfluorescent) is reduced resorufin (pink highly fluorescent) which further hydroresorufin (uncoloured nonfluorescent). It still not known how this occurs, intracellularly via enzyme activity or in medium a chemical reaction, although fluorescent form was found cytoplasm living...
Level 1: Overviews 2: Methods and Instrumentation 3: Practical Applications Appendices
We elucidate the detailed effects of gut microbial depletion on bile acid sub-metabolome multiple body compartments (liver, kidney, heart, and blood plasma) in rats. use a targeted ultra-performance liquid chromatography with time flight mass-spectrometry assay to characterize differential primary secondary profiles each tissue show major increase proportion taurine-conjugated acids germ-free (GF) antibiotic (streptomycin/penicillin)-treated Although conjugated dominate hepatic profile (97.0...
Inflammatory bowel diseases (IBD) including Crohn's disease (CD) and ulcerative colitis (UC) have a major impact on the health of individuals populations. Accurate diagnosis inflammatory at an early stage, correct differentiation between (UC), is important for optimum treatment prognosis. We present here first characterization fecal extracts obtained from patients with CD UC by employing noninvasive metabonomics approach, which combines high resolution 1H NMR spectroscopy multivariate...
A method for performing untargeted metabolomic analysis of human serum has been developed based on protein precipitation followed by Ultra Performance Liquid Chromatography and Time-of-Flight mass spectrometry (UPLC-TOF-MS). This was specifically designed to fulfill the requirements a long-term study, spanning more than 3 years, it subsequently thoroughly evaluated robustness repeatability. We describe here observed drift in instrumental performance over time its improvement with adjustment...
Self-evidently, research in areas supporting “systems biology” such as genomics, proteomics, and metabonomics are critically dependent on the generation of sound analytical data. Metabolic phenotyping using LC−MS-based methods is currently at a relatively early stage development, approaches to ensure data quality still developing. As part studies application LC−MS metabonomics, within-day reproducibility LC−MS, with both positive negative electrospray ionization (ESI), has been investigated...
A new approach to obtain fragmentation information in liquid chromatography/mass spectrometry (LC/MS) studies of small molecules complex mixtures is presented using simultaneous acquisition exact mass at high and low collision energy, MS(E). LC/MS-TOF LC/MS/MS-TOF are powerful tools for the analysis mixtures, especially those biological fluids allowing elucidation elemental composition information. In this example rat urine was studied approach, structures several endogenous components be...
The combination of a new 1.7 μm reversed-phase packing material, and chromatographic system, operating at ca. 12 000 psi, (so-called ultra performance liquid chromatography, UPLC) has enabled dramatic increases in to be obtained for complex mixture separation. This increase is manifested improved peak resolution, together with increased speed sensitivity. Here, we show that UPLC offers significant advantages over conventional HPLC amounting more than doubling capacity, an almost 10-fold 3-...