A5037616278- Metabolomics and Mass Spectrometry Studies
- Diet and metabolism studies
- Gut microbiota and health
- Liver Disease Diagnosis and Treatment
- Nutritional Studies and Diet
- Spectroscopy and Chemometric Analyses
- Analytical Chemistry and Chromatography
- Advanced Chemical Sensor Technologies
- Advanced MRI Techniques and Applications
- NMR spectroscopy and applications
- Metabolism and Genetic Disorders
- Traditional Chinese Medicine Studies
- Tryptophan and brain disorders
- Adipose Tissue and Metabolism
- Microbial Metabolic Engineering and Bioproduction
- Pelvic floor disorders treatments
- Clostridium difficile and Clostridium perfringens research
- Reproductive tract infections research
- Alcohol Consumption and Health Effects
- Bariatric Surgery and Outcomes
- Spectroscopy Techniques in Biomedical and Chemical Research
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Preterm Birth and Chorioamnionitis
- Parasites and Host Interactions
Imperial College London
2016-2025
Murdoch University
2019-2025
Harry Perkins Institute of Medical Research
2021-2025
Avenir Health
2019-2025
Hammersmith Hospital
2015-2024
University of Opole
2024
Imperial Valley College
2014-2024
Quadram Institute
1996-2024
Weatherford College
2024
Bridge University
2024
Abstract The characteristics of the OPLS method have been investigated for purpose discriminant analysis (OPLS‐DA). We demonstrate how class‐orthogonal variation can be exploited to augment classification performance in cases where individual classes exhibit divergence within‐class variation, analogy with soft independent modelling class (SIMCA) classification. prediction results will largely equivalent traditional supervised using PLS‐DA if no such is present classes. A discriminatory...
Humans have evolved intimate symbiotic relationships with a consortium of gut microbes (microbiome) and individual variations in the microbiome influence host health, may be implicated disease etiology, affect drug metabolism, toxicity, efficacy. However, molecular basis these microbe-host interactions roles bacterial species are obscure. We now demonstrate a"transgenomic" approach to link metabolic phenotype (metabotype) variation. used combination spectroscopic, microbiomic, multivariate...
Kwashiorkor, an enigmatic form of severe acute malnutrition, is the consequence inadequate nutrient intake plus additional environmental insults. To investigate role gut microbiome, we studied 317 Malawian twin pairs during first 3 years life. During this time, half remained well nourished, whereas 43% became discordant, and 7% manifested concordance for malnutrition. Both children in discordant kwashiorkor were treated with a peanut-based, ready-to-use therapeutic food (RUTF). Time-series...
Here, we study the intricate relationship between gut microbiota and host cometabolic phenotypes associated with dietary-induced impaired glucose homeostasis nonalcoholic fatty liver disease (NAFLD) in a mouse strain (129S6) known to be susceptible these traits, using plasma urine metabotyping, achieved by (1)H NMR spectroscopy. Multivariate statistical modeling of spectra shows that genetic predisposition 129S6 NAFLD is disruptions choline metabolism, i.e., low circulating levels...
We describe here the implementation of statistical total correlation spectroscopy (STOCSY) analysis method for aiding identification potential biomarker molecules in metabonomic studies based on NMR spectroscopic data. STOCSY takes advantage multicollinearity intensity variables a set spectra (in this case 1H spectra) to generate pseudo-two-dimensional spectrum that displays among intensities various peaks across whole sample. This is not limited usual connectivities are deducible from more...
We elucidate the detailed effects of gut microbial depletion on bile acid sub-metabolome multiple body compartments (liver, kidney, heart, and blood plasma) in rats. use a targeted ultra-performance liquid chromatography with time flight mass-spectrometry assay to characterize differential primary secondary profiles each tissue show major increase proportion taurine-conjugated acids germ-free (GF) antibiotic (streptomycin/penicillin)-treated Although conjugated dominate hepatic profile (97.0...
Inflammatory bowel diseases (IBD) including Crohn's disease (CD) and ulcerative colitis (UC) have a major impact on the health of individuals populations. Accurate diagnosis inflammatory at an early stage, correct differentiation between (UC), is important for optimum treatment prognosis. We present here first characterization fecal extracts obtained from patients with CD UC by employing noninvasive metabonomics approach, which combines high resolution 1H NMR spectroscopy multivariate...
In general, applications of metabonomics using biofluid NMR spectroscopic analysis for probing abnormal biochemical profiles in disease or due to toxicity have all relied on the use chemometric techniques sample classification. However, well-known variability some chemical shifts 1H spectra biofluids environmental differences such as pH variation, when coupled with large number variables spectra, has led situation where it is necessary reduce size attempt align shifting peaks, get more...
It is evident that the gut microbiota and factors influence its composition activity effect human metabolic, immunological developmental processes. We previously reported extreme physical with associated dietary adaptations, such as pursued by professional athletes, changes in faecal microbial diversity relative to of individuals a more sedentary lifestyle. Here we address impact these on functionality/metabolic which reveals even greater separation between exercise state.Metabolic...
Abstract The composition and structure of the pregnancy vaginal microbiome may influence susceptibility to adverse outcomes. Studies on pregnant have largely been limited Northern American populations. Using MiSeq sequencing 16S rRNA gene amplicons, we characterised microbiota a mixed British cohort women (n = 42) who experienced uncomplicated term delivery were sampled longitudinally throughout (8–12, 20–22, 28–30 34–36 weeks gestation) 6 postpartum. We show that dramatically changes...
Hydrazine is a model toxin that induces both hepatotoxic and neurotoxic effects in experimental animals. The direct biochemical of hydrazine kidney, liver, brain tissue were assessed male Sprague−Dawley rats using magic angle spinning nuclear magnetic resonance (NMR) spectroscopy. A single dose (90 mg/kg) resulted changes to the composition liver after 24 h including an increase triglycerides β-alanine, together with decrease hepatic glycogen, glucose, choline, taurine,...