A5011494588- Metabolomics and Mass Spectrometry Studies
- Liver Disease Diagnosis and Treatment
- Gut microbiota and health
- Lipid metabolism and disorders
- Diet, Metabolism, and Disease
- Neurotransmitter Receptor Influence on Behavior
- Clostridium difficile and Clostridium perfringens research
- Pharmacological Effects of Natural Compounds
- Advanced Chemical Sensor Technologies
- Psychedelics and Drug Studies
- Lipid metabolism and biosynthesis
- Forensic Toxicology and Drug Analysis
- Rabbits: Nutrition, Reproduction, Health
- Drug Transport and Resistance Mechanisms
- Coronary Interventions and Diagnostics
- Endoplasmic Reticulum Stress and Disease
- Atherosclerosis and Cardiovascular Diseases
- Cardiovascular Disease and Adiposity
- Nosocomial Infections in ICU
- Traditional Chinese Medicine Analysis
- Liver Disease and Transplantation
- Pancreatitis Pathology and Treatment
- Poisoning and overdose treatments
- Cancer, Lipids, and Metabolism
- Plant biochemistry and biosynthesis
Aristotle University of Thessaloniki
2019-2024
Centre for Research and Technology Hellas
2022
Detailed investigation of the lipidome remodeling upon normal weight conditions, obesity, or loss, as well influence physical activity, can help to understand mechanisms underlying dyslipidemia in metabolic conditions correlated emergence and progression non-alcoholic fatty liver disease (NAFLD). C57BL/6 male mice were fed a diet (ND) high-fat (HFD) for 20 weeks. Subgroups within group underwent different interventions: some engaged exercise (HFDex), others subjected loss (WL) by changing...
Recent studies support that acylcarnitines exert a significant role in cardiovascular disease development and progression. The aim of this metabolomics-based study was to investigate the association serum acylcarnitine levels with coronary artery (CAD) severity, as assessed via SYNTAX Score. Within context prospective CorLipid trial (NCT04580173), 13 circulating were accurately determined through newly developed HILIC-MS/MS method 958 patients undergoing angiography AHEPA University Hospital...
Nonalcoholic fatty liver disease (NAFLD), nowadays the most prevalent chronic in Western countries, is characterized by a variable phenotype ranging from steatosis to nonalcoholic steatohepatitis (NASH). Intracellular lipid accumulation considered hallmark of NAFLD and associated with lipotoxicity inflammation, as well increased oxidative stress levels. In this study, lipidomic approach was used investigate plasma lipidome 12 NASH patients, 10 Fatty Liver (NAFL) 15 healthy controls,...
Developing risk assessment tools for CAD prediction remains challenging nowadays. We developed an ML predictive algorithm based on metabolic and clinical data determining the severity of CAD, as assessed via SYNTAX score. Analytical methods were to determine serum blood levels specific ceramides, acyl-carnitines, fatty acids, proteins such galectin-3, adiponectin, APOB/APOA1 ratio. Patients grouped into: obstructive (SS > 0) non-obstructive = 0). A (boosted ensemble XGBoost) was by combining...
Patients with non-alcoholic steatohepatitis (NASH) show significantly faster progress in the stages of fibrosis compared to those fatty liver (NAFL) disease. The non-invasive diagnosis NASH remains an unmet clinical need. Preliminary data have shown that sphingolipids, especially ceramides, acids, and other lipid classes may be related presence histological activity aim our study was assess association certain plasma classes, such as acylcarnitines, histopathological findings patients NASH....
Background/Objectives: Lipid dysmetabolism seems to contribute the development and progression of nonalcoholic fatty liver disease (NAFLD). Our aim was compare serum lipidomic profile between patients with NAFLD having received monotherapy vitamin E (400 IU/d) those combination therapy low-dose spironolactone (25 mg/d) for 52 weeks. Methods: This a post hoc study randomized controlled trial (NCT01147523). Serum analysis performed in group (n = 15) plus 12). We employed an untargeted liquid...
Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) is the most common chronic liver disorder in Western countries, encompassing a range of conditions from steatosis to Steatohepatitis (MASH), which can potentially progress cirrhosis. Lipidomics approaches have revealed significant alterations hepatic lipidome associated with both and steatohepatitis, these changes correlating disease manifestation. While transition MASH remains poorly understood, recent research indicates that...
Clostridioides difficile infection (CDI) is responsible for an increasing number of cases post-antibiotic diarrhea worldwide, which has high severity and mortality among hospitalized elderly patients. The disruption gut microbiota due to antibacterial medication facilitates the intestinal colonization C. difficile. In present study, a murine model was used investigate potential effects antibiotic administration subsequent by difficile, as well three different 10-day treatments...
Adiponutrin (patatin-like phospholipase domain-containing 3; PNPLA3), encoded in humans by the PNPLA3 gene, is a protein associated with lipid droplet and endoplasmic reticulum membranes, where it apparently involved fatty acid redistribution between triglycerides phospholipids. A common polymorphism of (I148M, rs738409), linked to increased presence on droplets, strong genetic determinant non-alcoholic liver disease (NAFLD) its progression. P-glycoprotein (Pgp, MDR1—multidrug resistance 1,...
Abstract Lacosamide is a functionalized amino acid with antiepileptic function. Therapeutic drug monitoring ( TDM ) in patients for lacosamide critical as it allows clinicians to control epileptic seizures. A single liquid–liquid extraction step was applied the of from whole blood samples which were thereafter analyzed by GC ‐ MS . Optimum conditions selected on basis experiments various solvents at different pH s, indicating ethyl acetate 12 most efficient parameters lacosamide. Method...
Pregnant women are among the high-risk populations for COVID-19, whereas risk of vertical transmission to fetus is very low. Nevertheless, metabolic alternations described in COVID-19 patients may also occur pregnant and their offspring. We prospectively evaluated plasma lipidomic metabolomic profiles, soon after birth, neonates born infected mothers (cases, n = 10) offspring uninfected ones at delivery (controls, 10). All cases had two negative tests SARS-CoV-2 (nasopharyngeal swabs)...
Lipidomics emerges as a promising research field with the potential to help in personalized risk stratification and improve our understanding on functional role of individual lipid species metabolic perturbations occurring coronary artery disease (CAD). This study aimed utilize machine learning approach provide panel able identify patients obstructive CAD. In this posthoc analysis prospective CorLipid trial, we investigated profiles 146 suspected CAD, divided into two categories based...
Metabolic syndrome (MetS) is a complex condition characterized by fat accumulation, dyslipidemia, impaired glucose control and hypertension. In this study, rats were fed high-fat high-fructose (HFF) diet in order to develop MetS. After ten weeks, the dietary-induced MetS was confirmed higher body percentage, lower HDL-cholesterol increased blood pressure HFF-fed compared normal-fed animals. However, effect of development on lipidomic signature dietary-challenged remains be investigated. To...
Abstract Background Lipidomics emerge as a promising research field with the potential to help in personalized risk stratification and also improve our understanding on functional role of individual lipid species metabolic perturbations occurring coronary artery disease (CAD). The existing technological challenges detect diverse yet structurally similar lipids their isomers, well need for novel statistical computational tools handle high-dimensional lipidome may be responsible reduced...
The disruption of gut microbiota caused by antibiotics favors the intestinal colonization Clostridioides difficile – a Gram-positive, spore-forming anaerobic bacterium that causes potentially fatal gastrointestinal infections. In an endeavor to elucidate complexities gut-brain axis in context Clostridium infection (CDI), murine model has been used investigate potential effects antibiotic administration and subsequent C. difficile, as well impact three different 10-day treatments...
Background: A personalized, non-invasive assessment approach for evaluating the risk of obstructive coronary artery disease (CAD) is crucial patients with an intermediate or low clinical likelihood CAD before undergoing invasive angiography (ICA). This method allows clinicians to effectively rule out presence without need ICA determine if a referral warranted. Emerging lipidomics biomarkers may be valuable in this process. However, technological challenges detecting structurally similar...
Muscat of Alexandria is one the most aromatic grape cultivars, with a characteristic floral and fruity aroma, producing popular appellation origin wines. The winemaking process critical factor contributing to quality final product, so aim this work was study metabolomic changes during fermentation musts at industrial level from 11 tanks, 2 vintages, 3 wineries Limnos Island. A Headspace Solid-Phase Microextraction (HS-SPME) liquid injection Trimethylsilyl (TMS) derivatization Gas...
Urinary tract infections (UTI) of sows (characterized by ascending the urinary bladder (cyst), ureters, and renal pelvis), are major health issues with a significant economic impact to swine industry. The current detection UTI incidents lacks sensitivity; thus, UTIs remain largely under-diagnosed. value metabolomics in unraveling mechanisms sow has not yet been established. This study aims investigate urine metabolome for biomarkers. Urine samples were collected from 58 culled...