- Metabolomics and Mass Spectrometry Studies
- Aquaculture disease management and microbiota
- Aquaculture Nutrition and Growth
- Mercury impact and mitigation studies
- Traditional Chinese Medicine Studies
- Marine animal studies overview
- Heavy Metal Exposure and Toxicity
- Isotope Analysis in Ecology
- Advanced Chemical Sensor Technologies
- Coral and Marine Ecosystems Studies
- Aquatic Invertebrate Ecology and Behavior
- Meat and Animal Product Quality
- Physiological and biochemical adaptations
- Marine and coastal ecosystems
- Cephalopods and Marine Biology
- Marine Toxins and Detection Methods
- Neurobiology and Insect Physiology Research
- Heavy metals in environment
- Environmental Toxicology and Ecotoxicology
- Molecular Biology Techniques and Applications
- Identification and Quantification in Food
- Synthetic Organic Chemistry Methods
- Gut microbiota and health
- Marine Sponges and Natural Products
- Reproductive biology and impacts on aquatic species
National Institute of Standards and Technology
2013-2024
The Metabolomics Innovation Centre
2024
NCCOS Hollings Marine Laboratory
2009-2023
Material Measurement Laboratory
2023
Medical University of South Carolina
2009-2011
National Institute of Standards
2010
Tennessee Technological University
2009
Stockton University
2009
University of South Carolina
2007
Center for Environmental Health
2007
Recent progress in metabolomics and the development of increasingly sensitive analytical techniques have renewed interest global profiling, i.e., semiquantitative monitoring all chemical constituents biological fluids. In this work, we performed profiling NIST SRM 1950, "Metabolites Human Plasma", using GC-MS, LC-MS, NMR. Metabolome coverage, difficulties, reproducibility experiments on each platform are discussed. A total 353 metabolites been identified material. GC-MS provides 65 unique...
Success of the shrimp aquaculture industry requires technological advances that increase production and environmental sustainability. Indoor, superintensive, systems are being developed permit year-round farmed at high densities. These intended to overcome problems disease susceptibility water quality issues from waste products, by operating as essentially closed promote beneficial microbial communities (biofloc). The resulting biofloc can assimilate detoxify wastes, may provide nutrition...
Zebra mussel, Dreissena polymorpha, in the Great Lakes is being monitored as a bio-indicator organism for environmental health effects by National Oceanic and Atmospheric Administration's Mussel Watch program. In order to monitor of industrial pollution on ecosystem, invasive zebra mussels were collected from four stations-three inner harbor sites (LMMB4, LMMB1, LMMB) Milwaukee Estuary, one reference site (LMMB5) Lake Michigan, Wisconsin. Nuclear magnetic resonance (NMR)-based metabolomics...
Coral bleaching occurs when the symbioses between coral animals and their zooxanthellae is disrupted, either as part of a natural cycle or result unusual events. The bacterium Vibrio coralliilyticus (type strain ATCC BAA-450) has been linked to disease globally (for example in Mediterranean, Red Sea, Indian Ocean, Great Barrier Reef) like many other species exhibits temperature-dependent pathogenicity. temperature-dependence V. regard its metabolome was investigated. Nuclear magnetic...
The crustacean molting process is regulated by an interplay of hormones produced the eyestalk ganglia and Y-organs (YO). Molt-inhibiting hormone hyperglycemic released sinus gland (EG) inhibit synthesis secretion ecdysteroid YO, hence regulating hemolymph levels during molt cycle. purpose this study to investigate ecdysteroidogenesis pathway, specifically genes linked changes in occurring at early premolt (ePM). To end, a reference transcriptome based on EG, hepatopancreas was de novo...
Toxic trace element exposure occurs through release of the ubiquitous and naturally occurring elements arsenic (As), cadmium (Cd), lead (Pb), mercury (Hg). The unique environmental conditions wetland ecosystems along southeastern Atlantic coast United States to accumulation Hg which is greater than in most other country. There are also point sources As, Cd, Pb this region. To effectively monitor concentrations, consequently potential human exposure, accessible local sentinel species needed....
Trichodesmium is a suspected toxin-producing nonheterocystous cyanobacteria ubiquitous in tropical, subtropical, and temperate seas. The genus known for its ability to fix nitrogen form massive blooms. In oligotrophic seas, it can dominate the biomass be major component of oceanic primary production global cycling. Numerous reports suggest Trichodesmium-derived toxins are cause death fish, crabs, bivalves. Laboratory studies have demonstrated neurotoxic effects T. thiebautii cell extracts...
The field of metabolomics generally lacks standardized methods for the preparation samples prior to analysis. This is especially true reef-building corals, where handful studies that were published employ a range sample protocols. utilization may prove essential in understanding coral biology face increasing environmental threats, and an optimized method preparing analysis would aid this cause. current study evaluates three important steps during processing stony corals: (i) metabolite...
Abstract Coral growth anomalies (GAs) are tumor-like lesions that detrimental to colony fitness and commonly associated with high human population density, yet little is known about the disease pathology or calcification behavior. SEM imagery, skeletal trace elements boron isotopes (δ 11 B) have been combined as a novel approach study coral disease. Low Mg/Ca, U/Ca, Mo/Ca, V/Ca potentially suggest decreased abundance of “centers calcification” nitrogen-fixation in GAs. Estimates carbonate...