A5060926002- Metabolomics and Mass Spectrometry Studies
- Aquaculture disease management and microbiota
- Environmental Toxicology and Ecotoxicology
- Aquaculture Nutrition and Growth
- Microbial Natural Products and Biosynthesis
- Isotope Analysis in Ecology
- Advanced Chemical Sensor Technologies
- Electron Spin Resonance Studies
- Physiological and biochemical adaptations
- Fullerene Chemistry and Applications
- Sulfur Compounds in Biology
- Carbohydrate Chemistry and Synthesis
- Marine Biology and Ecology Research
- NMR spectroscopy and applications
- Aquatic Invertebrate Ecology and Behavior
- Analytical Chemistry and Chromatography
- Advanced NMR Techniques and Applications
- Redox biology and oxidative stress
- Spectroscopy and Chemometric Analyses
- Synthetic Organic Chemistry Methods
- Marine and fisheries research
- Diamond and Carbon-based Materials Research
- Graphene research and applications
- Polysaccharides and Plant Cell Walls
- Advanced MRI Techniques and Applications
NCCOS Hollings Marine Laboratory
2011-2023
National Institute of Standards and Technology
2011-2023
National Institute of Standards
2010-2014
Stockton University
2009
Medical University of South Carolina
1999-2009
Tennessee Technological University
2009
University of Birmingham
2008
Imperial College London
2008
NOAA National Ocean Service
1999-2007
Environmental Protection Agency
2002
Cancer-associated thrombocytosis has long been linked to poor clinical outcome, but the underlying mechanism is enigmatic. We hypothesized that platelets promote malignancy and resistance therapy by dampening host immunity. show genetic targeting of enhances adoptive T cell cancer. An unbiased biochemical structural biology approach established transforming growth factor β (TGFβ) lactate as major platelet-derived soluble factors obliterate CD4+ CD8+ functions. Moreover, we found are dominant...
Recent progress in metabolomics and the development of increasingly sensitive analytical techniques have renewed interest global profiling, i.e., semiquantitative monitoring all chemical constituents biological fluids. In this work, we performed profiling NIST SRM 1950, "Metabolites Human Plasma", using GC-MS, LC-MS, NMR. Metabolome coverage, difficulties, reproducibility experiments on each platform are discussed. A total 353 metabolites been identified material. GC-MS provides 65 unique...
In this commentary we present the findings from an international consortium on fish toxicogenomics sponsored by U.K. Natural Environment Research Council (Fish Toxicogenomics-Moving into Regulation and Monitoring, held 21-23 April 2008 at Pacific Environmental Science Centre, Vancouver, BC, Canada).The government agencies, academia, industry addressed three topics: progress in ecotoxicogenomics, regulatory perspectives roadblocks for practical implementation of risk assessment, dealing with...
Several fundamental requirements must be met so that NMR-based metabolomics and the related technique of metabonomics can formally adopted into environmental monitoring chemical risk assessment. Here we report an intercomparison exercise which has evaluated effectiveness 1H NMR to generate comparable data sets from environmentally derived samples. It focuses on laboratory practice follows sample collection metabolite extraction, specifically final stages preparation, (500, 600, 800 MHz),...
The nanoscale parameters of metal clusters and lattices have a crucial influence on the macroscopic properties materials. Herein, we provide detailed study size shape isolated yttrium carbide in different fullerene cages. A family diyttrium endohedral metallofullerenes with general formula Y(2)C(2n) (n = 40-59) are reported. high field (13)C nuclear magnetic resonance (NMR) density functional theory (DFT) methods employed to examine this cluster certain members, Y(2)C(2)@D(5)(450)-C(100),...
Success of the shrimp aquaculture industry requires technological advances that increase production and environmental sustainability. Indoor, superintensive, systems are being developed permit year-round farmed at high densities. These intended to overcome problems disease susceptibility water quality issues from waste products, by operating as essentially closed promote beneficial microbial communities (biofloc). The resulting biofloc can assimilate detoxify wastes, may provide nutrition...
β-glucan is a (1→3)-β-linked glucose polymer with (1→6)-β-linked side chains and major component of fungal cell walls. β-glucans provide structural integrity to the wall. The nature (1–6)-β-linked chain structure (1→3,1→6)-β-D-glucans has been very difficult elucidate. Herein, we report first detailed characterization Candida glabrata using high-field NMR. have an average length 4 5 repeat units spaced every 21 along (1→3)-linked backbone. Computer modeling suggests that bent curve allows...
Zebra mussel, Dreissena polymorpha, in the Great Lakes is being monitored as a bio-indicator organism for environmental health effects by National Oceanic and Atmospheric Administration's Mussel Watch program. In order to monitor of industrial pollution on ecosystem, invasive zebra mussels were collected from four stations-three inner harbor sites (LMMB4, LMMB1, LMMB) Milwaukee Estuary, one reference site (LMMB5) Lake Michigan, Wisconsin. Nuclear magnetic resonance (NMR)-based metabolomics...
A multiyear study in the C-111 canal system and associated sites Florida Bay was undertaken to determine potential pesticide risk that exists South Florida. After examination of extensive concentration data surface water, tissues, semipermeable membrane devices (SPMDs), contamination seems be derived from agricultural production drains into canal. The results this indicate runoff processes led quantifiable residues both bay which occasionally exceeded current water quality criteria. major...
Coral bleaching occurs when the symbioses between coral animals and their zooxanthellae is disrupted, either as part of a natural cycle or result unusual events. The bacterium Vibrio coralliilyticus (type strain ATCC BAA-450) has been linked to disease globally (for example in Mediterranean, Red Sea, Indian Ocean, Great Barrier Reef) like many other species exhibits temperature-dependent pathogenicity. temperature-dependence V. regard its metabolome was investigated. Nuclear magnetic...
Mithramycin is an aureolic acid-type antimicrobial and antitumor agent produced by Streptomyces argillaceus. Modifying post-polyketide synthase (PKS) tailoring enzymes involved in the production of mithramycin effective way gaining further information regarding late steps its biosynthetic pathway. In addition, new "unnatural" natural products class are likely to be produced. The role two such post-PKS enzymes, encoded mtmC mtmTIII, was investigated, four novel acid drugs, premithramycin-type...
We investigated the metabolic effects of four different commercial soy-based protein products on red drum fish (Sciaenops ocellatus) using nuclear magnetic resonance (NMR) spectroscopy-based metabolomics along with unsupervised principal component analysis (PCA) to evaluate profiles in liver, muscle, and plasma tissues. Specifically, during a 12 week feeding trial, juvenile maintained an indoor recirculating aquaculture system were fed commercially available soy formulations, containing same...
A targeted search for glycosyltransferase (GT) encoding genes in the gene cluster of urdamycin producer Streptomyces fradiae Tü2717 resulted discovery urdGT2, a GT located approximately 7 kb downstream minimal polyketide synthase (PKS) genes. Subsequent inactivation this created mutant strain, which produces completely different metabolites than wild-type consisting three new urdamycins I, J, and K. Their structures provide insight into important C-glycosyl-transfer step biosynthetic...