Richard F. Helm

ORCID: 0000-0001-5317-0925
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About
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Research Areas
  • Lignin and Wood Chemistry
  • Software Engineering Research
  • Biocrusts and Microbial Ecology
  • Carbohydrate Chemistry and Synthesis
  • Phytase and its Applications
  • Plant Gene Expression Analysis
  • Plant biochemistry and biosynthesis
  • Plant nutrient uptake and metabolism
  • Fermentation and Sensory Analysis
  • Advanced Software Engineering Methodologies
  • Polysaccharides and Plant Cell Walls
  • Algal biology and biofuel production
  • Biofuel production and bioconversion
  • Legume Nitrogen Fixing Symbiosis
  • Plant Micronutrient Interactions and Effects
  • Microbial Community Ecology and Physiology
  • Soybean genetics and cultivation
  • Biochemical and biochemical processes
  • Genetics and Neurodevelopmental Disorders
  • Bioinformatics and Genomic Networks
  • Microbial Metabolites in Food Biotechnology
  • Photosynthetic Processes and Mechanisms
  • Ubiquitin and proteasome pathways
  • Plant Molecular Biology Research
  • Glycosylation and Glycoproteins Research

Virginia Tech
2015-2024

Virginia–Maryland College of Veterinary Medicine
2019

University of Virginia
2019

Bruker (United States)
2019

Edward Via College of Osteopathic Medicine
2019

U.S. Dairy Forage Research Center
1992-2013

University of Wisconsin–Madison
1986-2011

Virginia Tech - Wake Forest University School of Biomedical Engineering & Sciences
2011

Wake Forest University
2011

Moffitt Cancer Center
2011

Behavioral compositions, groups of interdependent objects cooperating to accomplish tasks, are an important feature object-oriented systems. This paper introduces Contracts, a new technique for specifying behavioral compositions and the obligations on participating objects. Refinement composition contracts allows creation large grain abstractions based behavior, orthogonal those provided by existing class constructs. Using thus provides basis vocabulary Interaction-Oriented design which...

10.1145/97945.97967 article EN 1990-01-01

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPathway of p-Coumaric Acid Incorporation into Maize Lignin As Revealed by NMRJohn Ralph, Ronald D. Hatfield, Stephane Quideau, Richard F. Helm, John H. Grabber, and Hans-Joachim G. JungCite this: J. Am. Chem. Soc. 1994, 116, 21, 9448–9456Publication Date (Print):October 1, 1994Publication History Published online1 May 2002Published inissue 1 October...

10.1021/ja00100a006 article EN Journal of the American Chemical Society 1994-10-01

Auxin and ethylene are key regulators of plant growth development, thus the transcriptional networks that mediate responses to these hormones have been subject intense research. This study dissected hormonal cross talk regulating synthesis flavonols examined their impact on root development. We analyzed effects auxin an precursor roots wild-type hormone-insensitive Arabidopsis (Arabidopsis thaliana) mutants at transcript, protein, metabolite levels high spatial temporal resolution....

10.1104/pp.111.172502 article EN cc-by PLANT PHYSIOLOGY 2011-03-22

With widespread detection of endocrine disrupting compounds including hormones in wastewater, there is a need to develop cost-effective remediation technologies for their removal from wastewater. Previous research has shown that Fe3+-saturated montmorillonite effective quickly transforming phenolic organic such as pentachlorophenol, acids, and triclosan via surface-catalyzed oligomerization. However, little known about its effectiveness reaction mechanisms when reacting with hormones. In...

10.1021/es504815t article EN Environmental Science & Technology 2014-12-12

Methyl 5-O-(E)-[γ-13C]feruloyl-α-L-arabinofuranoside (FA-Ara) has been synthesized and incorporated into a synthetic lignin dehydrogenation polymer (DHP) of coniferyl alcohol. Inverse-detected long-range C–H correlation NMR experiments on the DHP gave peaks indicative copolymerization FA-Ara alcohol polymer. The bonding sites modes, as determined by analysis carbonyl region correlated 2D experiment, are predictable from free-radical coupling mechanisms. In addition to abundant 4-O-α′ 4-O-β′...

10.1039/p19920002961 article EN Journal of the Chemical Society. Perkin transactions I/Journal of the Chemical Society. Perkin transactions. I 1992-01-01

article Free Access Share on Visualizing the behavior of object-oriented systems Authors: Wim De Pauw View Profile , Richard Helm Doug Kimelman John Vlissides Authors Info & Claims ACM SIGPLAN NoticesVolume 28Issue 10Oct. 1, 1993 pp 326–337https://doi.org/10.1145/167962.165919Published:01 October 1993Publication History 89citation991DownloadsMetricsTotal Citations89Total Downloads991Last 12 Months94Last 6 weeks9 Get Citation AlertsNew Alert added!This alert has been successfully added and...

10.1145/165854.165919 article EN 1993-10-01

ABSTRACT Active Fe-superoxide dismutase (SodF) was the third most abundant soluble protein in cells of Nostoc commune CHEN/1986 after prolonged (13 years) storage desiccated state. Upon rehydration, Fe-containing superoxide disumutase (Fe-SOD) released and activity distributed between rehydrating extracellular fluid. The 21-kDa Fe-SOD polypeptide purified, N terminus sequenced, data were used to isolate sodF from clonal N. DRH1. encodes an open reading frame 200 codons is expressed as a...

10.1128/jb.182.1.189-197.2000 article EN Journal of Bacteriology 2000-01-01

ABSTRACT The structure of the viscous extracellular polysaccharide (glycan) desiccation-tolerant Nostoc commune DRH-1 was determined through chromatographic and spectroscopic methods. is novel in that it possesses a 1-4-linked xylogalactoglucan backbone with d -ribofuranose 3- O -[( R )-1-carboxyethyl]- -glucuronic acid (nosturonic acid) pendant groups. presence -ribose nosturonic as peripheral groups unusual, their potential roles modulating rheological properties glycan are discussed....

10.1128/jb.182.4.974-982.2000 article EN Journal of Bacteriology 2000-02-15

10.1023/a:1007965229567 article EN Journal of Applied Phycology 1997-01-01

The accepted derivation of lignins from non(enzymatically)-controlled radical coupling reactions has been recently challenged, and it is relevant to ascertain unequivocally whether are or not (as normally assumed) optically active. Two approaches were used. First, DFRC (derivatization followed by reductive cleavage) dimers derived β-5- β-β-units in pine lignins, which certainly retain unaltered chiral centers well as β-1- β-O-4-units where the intactness may be debated), shown inactive...

10.1021/jf9901136 article EN Journal of Agricultural and Food Chemistry 1999-07-07

Bacterial chemotaxis is an important attribute that aids in establishing symbiosis between rhizobia and their legume hosts. Plant roots seeds exude a spectrum of molecules into the soil to attract bacterial symbionts. The alfalfa symbiont Sinorhizobium meliloti possesses eight chemoreceptors sense its environment mediate toward host. methyl accepting protein McpU one more abundant S. sensor for potent attractant proline. We established dominant role sensing exuded by seeds. Mass spectrometry...

10.1128/aem.00115-14 article EN Applied and Environmental Microbiology 2014-03-22

Given that cellular O-GlcNAcylation levels are thought to be real-time measures of nutrient status and dysregulated O-GlcNAc signaling is associated with insulin resistance, we evaluated the role transferase (OGT), enzyme mediates O-GlcNAcylation, in skeletal muscle. We assessed muscle from obese, type 2 diabetic people, characterized muscle-specific OGT knockout (mKO) mice metabolic cages measured energy expenditure substrate utilization pattern using indirect calorimetry. Whole body...

10.1016/j.molmet.2018.02.010 article EN cc-by-nc-nd Molecular Metabolism 2018-02-24

Amyloid formation of the 37-residue amylin is involved in pathogenesis type 2 diabetes and, potentially, diabetes-induced neurological deficits. Numerous flavonoids exhibit inhibitory effects against amyloidosis, but mechanisms inhibition remain unclear. Screening a library natural compounds uncovered potent lead compound, flavone baicalein. Baicalein inhibits amyloid and reduces amylin-induced cytotoxicity. Analogue analyses demonstrated, for first time, key roles vicinal hydroxyl groups on...

10.1021/acs.biochem.6b00578 article EN Biochemistry 2016-07-19

ABSTRACT A transcriptional analysis of the response Saccharomyces cerevisiae strain BY4743 to controlled air-drying (desiccation) and subsequent rehydration under minimal glucose conditions was performed. Expression genes involved in fatty acid oxidation glyoxylate cycle observed increase during drying remained this state phase. When expression profile for dried sample compared that a commercially prepared dry active yeast, strikingly similar changes were observed. The fact these two...

10.1128/aem.71.12.8752-8763.2005 article EN Applied and Environmental Microbiology 2005-12-01

We present an unusual algorithm involving classification trees---CARTwheels---where two trees are grown in opposite directions so that they joined at their leaves. This approach finds application a new data mining task we formulate, called redescription mining. A is shift-of-vocabulary, or different way of communicating information about given subset data; the goal to find subsets afford multiple descriptions. highlight importance this problem domains such as bioinformatics, which exhibit...

10.1145/1014052.1014083 article EN 2004-08-22

Behavioral compositions, groups of interdependent objects cooperating to accomplish tasks, are an important feature object-oriented systems. This paper introduces Contracts , a new technique for specifying behavioral compositions and the obligations on participating objects. Refinement composition contracts allows creation large grain abstractions based behavior, orthogonal those provided by existing class constructs. Using thus provides basis vocabulary Interaction-Oriented design which...

10.1145/97946.97967 article EN ACM SIGPLAN Notices 1990-09-01
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