A5012899764- Metabolomics and Mass Spectrometry Studies
- Spectroscopy and Chemometric Analyses
- Advanced Proteomics Techniques and Applications
- Analytical Chemistry and Chromatography
- Pregnancy and preeclampsia studies
- Birth, Development, and Health
- Advanced Chemical Sensor Technologies
- Microbial Metabolic Engineering and Bioproduction
- Gut microbiota and health
- Diet and metabolism studies
- Mass Spectrometry Techniques and Applications
- Analytic Number Theory Research
- Traditional Chinese Medicine Studies
- Gestational Diabetes Research and Management
- Cancer Risks and Factors
- Advanced Mathematical Identities
- Nutrition, Genetics, and Disease
- Venous Thromboembolism Diagnosis and Management
- Computational Drug Discovery Methods
- Nutritional Studies and Diet
- Gene expression and cancer classification
- Neonatal and fetal brain pathology
- Black Holes and Theoretical Physics
- Bioinformatics and Genomic Networks
- Blood Coagulation and Thrombosis Mechanisms
Edith Cowan University
2016-2025
The Open University
2008-2022
ETH Zurich
2022
ETH Zürich Foundation
2022
University of Manchester
2005-2016
Czech Academy of Sciences, Institute of Biotechnology
2016
University of Alberta
2010-2016
Alberta Hospital Edmonton
2015
University College Cork
2009-2012
Cork University Hospital
2009-2012
First released in 2009, MetaboAnalyst (www.metaboanalyst.ca) was a relatively simple web server designed to facilitate metabolomic data processing and statistical analysis. With continuing advances metabolomics along with constant user feedback, it became clear that substantial upgrade the original necessary. 2.0, which is successor MetaboAnalyst, represents just such an upgrade. 2.0 now contains dozens of new features functions including procedures for filtering, editing normalization. It...
Metabolomics is increasingly being applied towards the identification of biomarkers for disease diagnosis, prognosis and risk prediction. Unfortunately among many published metabolomic studies focusing on biomarker discovery, there very little consistency relatively rigor in how researchers select, assess or report their candidate biomarkers. In particular, few any measure sensitivity, specificity, provide receiver operator characteristic (ROC) curves with associated confidence intervals....
A method for performing untargeted metabolomic analysis of human serum has been developed based on protein precipitation followed by Ultra Performance Liquid Chromatography and Time-of-Flight mass spectrometry (UPLC-TOF-MS). This was specifically designed to fulfill the requirements a long-term study, spanning more than 3 years, it subsequently thoroughly evaluated robustness repeatability. We describe here observed drift in instrumental performance over time its improvement with adjustment...
Metabolomics is the comprehensive study of metabolome, repertoire biochemicals (or small molecules) present in cells, tissues, and body fluids. The metabolism at global or "-omics" level a rapidly growing field that has potential to have profound impact upon medical practice. At center metabolomics, concept person's metabolic state provides close representation individual's overall health status. This reflects what been encoded by genome, modified diet, environmental factors, gut microbiome....
Metabolomics and systems biology require the acquisition of reproducible, robust, reliable, homogeneous biological data sets. Therefore, we developed validated standard operating procedures (SOPs) for quenching efficient extraction metabolites from Escherichia coli to determine best methods approach global analysis metabolome. E. was grown in chemostat culture so that cellular metabolism could be held steady-state conditions under a range precisely defined growth conditions, thus enabling...
Preeclampsia is a pregnancy-specific syndrome that causes substantial maternal and fetal morbidity mortality. The etiology incompletely understood, there no clinically useful screening test. Current metabolomic technologies have allowed the establishment of metabolic signatures preeclampsia in early pregnancy. Here, 2-phase discovery/validation profiling study was performed. In discovery phase, nested case-control designed, using samples obtained at 15±1 weeks’ gestation from 60 women who...
Phenotyping of 1,200 'healthy' adults from the UK has been performed through investigation diverse classes hydrophilic and lipophilic metabolites present in serum by applying a series chromatography-mass spectrometry platforms. These data were made robust to instrumental drift numerical correction; this was prerequisite allow detection subtle metabolic differences. The variation observed metabolite relative concentrations between subjects ranged less than 5 % more 200 %. Variations could be...
Human immunodeficiency virus type 1(HIV-1) infects and activates innate immune cells in the brain resulting inflammation neuronal death with accompanying neurological deficits. Induction of inflammasomes causes cleavage release IL-1β IL-18, representing pathogenic processes that underlie inflammatory diseases although their contribution HIV-associated disease is unknown. Investigation inflammasome-associated genes revealed IL-1β, IL-18 caspase-1 were induced brains HIV-infected persons...
ABSTRACT Fourier transform infrared (FT-IR) spectroscopy is a rapid, noninvasive technique with considerable potential for application in the food and related industries. We show here that this can be used directly on surface of to produce biochemically interpretable “fingerprints.” Spoilage meat result decomposition formation metabolites caused by growth enzymatic activity microorganisms. FT-IR was exploited measure biochemical changes within substrate, enhancing accelerating detection...
Abstract Background Cell growth underlies many key cellular and developmental processes, yet a limited number of studies have been carried out on cell-growth regulation. Comprehensive at the transcriptional, proteomic metabolic levels under defined controlled conditions are currently lacking. Results Metabolic control analysis is being exploited in systems biology study eukaryotic cell. Using chemostat culture, we measured impact changes flux (growth rate) transcriptome, proteome,...
The chemical identification of mass spectrometric signals in metabolomic applications is important to provide conversion analytical data biological knowledge about metabolic pathways. complexity electrospray acquired from a range samples (serum, urine, yeast intracellular extracts, footprints, placental tissue footprints) has been investigated and defined the frequency different ion types routinely detected. Although some were expected (protonated deprotonated peaks, isotope multiply charged...
There has been considerable progress recently towards developing therapeutic strategies for Huntington's disease (HD), with several compounds showing beneficial effects in transgenic mouse models. However, human trials HD are difficult, costly and time-consuming due to the slow course, insidious onset patient-to-patient variability. Identification of molecular biomarkers associated progression will aid development effective therapies by allowing further validation animal models providing...
A method for the preparation and GC−TOF-MS analysis of human serum samples has been developed evaluated application in long-term metabolomic studies. Serum were deproteinized using 3:1 methanol/serum, dried a vacuum concentrator, chemically derivatized two-stage process. Samples analyzed by with 25 min time. In addition, quality control (QC) used to quantify process variability. Optimization chemical derivatization was performed. Products found be stable 30 h after derivatization. An...