A5025708477- Metabolomics and Mass Spectrometry Studies
- Advanced Chemical Sensor Technologies
- Mass Spectrometry Techniques and Applications
- Alzheimer's disease research and treatments
- Trace Elements in Health
- Atmospheric chemistry and aerosols
- Atmospheric Ozone and Climate
- Analytical Chemistry and Chromatography
- Genetic Neurodegenerative Diseases
- Mitochondrial Function and Pathology
- Atmospheric and Environmental Gas Dynamics
- Heavy Metal Exposure and Toxicity
- Infant Nutrition and Health
- Metabolism and Genetic Disorders
- Birth, Development, and Health
- Neurological diseases and metabolism
- Cancer, Lipids, and Metabolism
- Gestational Diabetes Research and Management
- Neurological Disorders and Treatments
- Climate change impacts on agriculture
- Drug-Induced Ocular Toxicity
- Neuroscience and Neuropharmacology Research
- Ruminant Nutrition and Digestive Physiology
- Agriculture, Soil, Plant Science
- Alcohol Consumption and Health Effects
University of Manchester
2009-2020
Manchester Academic Health Science Centre
2015-2020
Manchester University NHS Foundation Trust
2015-2017
Cardiovascular Research Center
2017
NIHR Manchester Biomedical Research Centre
2015
University of East Anglia
2007-2013
AstraZeneca (United Kingdom)
2009
University of Bern
2007
Natural Environment Research Council
2007
British Antarctic Survey
2007
A method for performing untargeted metabolomic analysis of human serum has been developed based on protein precipitation followed by Ultra Performance Liquid Chromatography and Time-of-Flight mass spectrometry (UPLC-TOF-MS). This was specifically designed to fulfill the requirements a long-term study, spanning more than 3 years, it subsequently thoroughly evaluated robustness repeatability. We describe here observed drift in instrumental performance over time its improvement with adjustment...
Phenotyping of 1,200 'healthy' adults from the UK has been performed through investigation diverse classes hydrophilic and lipophilic metabolites present in serum by applying a series chromatography-mass spectrometry platforms. These data were made robust to instrumental drift numerical correction; this was prerequisite allow detection subtle metabolic differences. The variation observed metabolite relative concentrations between subjects ranged less than 5 % more 200 %. Variations could be...
The chemical identification of mass spectrometric signals in metabolomic applications is important to provide conversion analytical data biological knowledge about metabolic pathways. complexity electrospray acquired from a range samples (serum, urine, yeast intracellular extracts, footprints, placental tissue footprints) has been investigated and defined the frequency different ion types routinely detected. Although some were expected (protonated deprotonated peaks, isotope multiply charged...
A method for the preparation and GC−TOF-MS analysis of human serum samples has been developed evaluated application in long-term metabolomic studies. Serum were deproteinized using 3:1 methanol/serum, dried a vacuum concentrator, chemically derivatized two-stage process. Samples analyzed by with 25 min time. In addition, quality control (QC) used to quantify process variability. Optimization chemical derivatization was performed. Products found be stable 30 h after derivatization. An...
Alzheimer's disease (AD) is an age-related neurodegenerative disorder that displays pathological characteristics including senile plaques and neurofibrillary tangles. Metabolic defects are also present in AD-brain: for example, signs of deficient cerebral glucose uptake may occur decades before onset cognitive dysfunction tissue damage. There have been few systematic studies the metabolite content AD human brain, possibly due to scarcity high-quality brain and/or lack reliable experimental...
Widespread brain-copper deficiency in patients with Alzheimer's dementia (red) compared to controls (blue).
High glucose levels in the peripheral nervous system (PNS) have been implicated pathogenesis of diabetic neuropathy (DN). However, our understanding molecular mechanisms that cause marked distal pathology is incomplete. We performed a comprehensive, system-wide analysis PNS rodent model DN. integrated proteomics and metabolomics from sciatic nerve (SN), lumbar 4/5 dorsal root ganglia (DRG), trigeminal (TG) streptozotocin-diabetic healthy control rats. Even though all tissues showed dramatic...
Abstract Impairment of brain-glucose uptake and brain-copper regulation occurs in Alzheimer’s disease (AD). Here we sought to further elucidate the processes that cause neurodegeneration AD by measuring levels metabolites metals brain regions undergo different degrees damage. We employed mass spectrometry (MS) measure seven post-mortem nine patients controls, plasma-glucose plasma-copper an ante-mortem case-control study. Glucose, sorbitol fructose were markedly elevated all regions, whereas...
Huntington's disease (HD) is a neurodegenerative disorder caused by an expanded CAG repeat in exon 1 of the
Huntington's disease (HD) is a genetically-mediated neurodegenerative disorder wherein the aetiological defect mutation in gene (HTT), which alters structure of huntingtin protein (Htt) through lengthening its polyglutamine tract, thus initiating cascade that ultimately leads to premature death. However, neurodegeneration typically manifests HD only middle age, and mechanisms linking causative brain are poorly understood. Brain metabolism severely perturbed HD, some studies have indicated...
The atmospheric histories of two potent greenhouse gases, tetrafluoromethane (CF4) and hexafluoroethane (C2F6), have been reconstructed for the 20th century based on firn air measurements from both hemispheres. trends show that mixing ratios CF4 C2F6 increased during by factors ∼2 ∼10, respectively. Initially, increasing coincided with rise in primary aluminum production. However, a slower growth rate appears to be evident 1990s, which supports recent industry reports reduced emissions. This...
Objective: No single diagnostic investigation is currently available for necrotising enterocolitis (NEC). We implemented a novel, untargeted, exploratory study to determine whether metabolomics can reveal early biomarker(s) of NEC. The effect gestational age on the metabolome was also investigated.
The impact of diabetes mellitus on the central nervous system is less widely studied than in peripheral system, but there increasing evidence that it elevates risk developing cognitive deficits. aim this study was to characterize experimental proteome and metabolome hippocampus. We tested hypothesis vitamin B6 isoform pyridoxamine protective against functional molecular changes diabetes.
Ob/ob mice provide an animal model for non-alcoholic fatty liver disease/non-alcoholic steatohepatitis (NAFLD/NASH) in patients with obesity and type-2 diabetes. Low copper has been linked to hepatic lipid build-up (steatosis) animals systemic deficiency caused by low-copper diets. However, status NAFLD or NASH is uncertain, a validated useful the study of regulation common forms metabolic disease lacking. Here, we report parallel measurements essential metal levels whole-liver tissue...
Abstract. The atmospheric records of four halons, H-1211 (CBrClF2), H-1301 (CBrF3), H-2402 (CBrF2CBrF2) and H-1202 (CBr2F2), measured from air collected at Cape Grim, Tasmania, between 1978 2011, are reported. Mixing ratios H-1211, began to decline in the early mid-2000s, but those continue increase up mid-2011. These trends compared reported by NOAA (National Oceanic Atmospheric Administration) AGAGE (Advanced Global Experiment). observations suggest that contribution halons total...
Abstract The specific consequences of hyperglycaemia on placental metabolism and function are incompletely understood but likely contribute to poor pregnancy outcomes associated with diabetes mellitus (DM). This study aimed identify the functional biochemical pathways perturbed by exposure high glucose levels through integrative analysis trophoblast transcriptome metabolome. human cell line, BeWo, was cultured in 5 or 25 mM glucose, as a model placenta DM. Transcriptomic using microarrays,...
Sporadic Alzheimer's disease (AD) is a neurodegenerative disorder that causes the most prevalent form of age-related dementia but its pathogenesis remains obscure. Altered regulation metals, particularly pan-cerebral copper deficiency, and more regionally-localized perturbation other are prominent in AD brain although data on how these CNS perturbations reflected peripheral bloodstream inconsistent to date. To assess potential use metal dysregulation generate biomarkers AD, we performed...