A5066443529- Pregnancy and preeclampsia studies
- Birth, Development, and Health
- Reproductive System and Pregnancy
- Pregnancy and Medication Impact
- Neonatal and fetal brain pathology
- Maternal and fetal healthcare
- Neonatal Respiratory Health Research
- Gestational Diabetes Research and Management
- Prenatal Screening and Diagnostics
- Extracellular vesicles in disease
- Cardiovascular Issues in Pregnancy
- Blood Coagulation and Thrombosis Mechanisms
- Preterm Birth and Chorioamnionitis
- Phonocardiography and Auscultation Techniques
- Maternal and Perinatal Health Interventions
- MicroRNA in disease regulation
- Selenium in Biological Systems
- COVID-19 Impact on Reproduction
- Non-Invasive Vital Sign Monitoring
- Cell Adhesion Molecules Research
- Parvovirus B19 Infection Studies
- Assisted Reproductive Technology and Twin Pregnancy
- Global Maternal and Child Health
- IL-33, ST2, and ILC Pathways
- Infant Nutrition and Health
John Radcliffe Hospital
2013-2024
University of Oxford
2015-2024
Cincinnati Children's Hospital Medical Center
2018
Nuffield Orthopaedic Centre
1983-2018
Brunel University of London
2017
University of Reading
2017
Karolinska University Hospital
2017
Karolinska Institutet
2017
University of Tampa
2017
Sahlgrenska University Hospital
2017
Cellular microvesicles and nanovesicles (exosomes) are involved in many disease processes have major potential as biomarkers. However, developments this area constrained by limitations the technology available for their measurement. Here we report on use of fluorescence nanoparticle tracking analysis (NTA) to rapidly size phenotype cellular vesicles. In system vesicles visualized light scattering using a microscope. A video is taken, NTA software tracks brownian motion individual calculates...
Preeclampsia is a serious complication of pregnancy in which the fetus receives an inadequate supply blood due to failure trophoblast invasion. There evidence that condition has immunological basis. The only known polymorphic histocompatibility antigens on fetal are HLA-C molecules. We tested idea recognition these molecules by killer immunoglobulin receptors (KIRs) maternal decidual NK cells key factor development preeclampsia. Striking differences were observed when ligand: receptor pairs...
To measure the incidence of eclampsia, establish how often it is preceded by signs pre-eclampsia, document morbidity associated with and determine maternal case fatality rates.A prospective, descriptive study every eclampsia in United Kingdom 1992. Information was collected from reviews hospital notes questionnaires to general practitioners.All 279 hospitals a consultant obstetric unit.Obstetricians midwives notified 582 possible cases, 383 were confirmed as eclampsia. The national...
The ability to determine fetal RhD status noninvasively is useful in the treatment of RhD-sensitized pregnant women whose partners are heterozygous for gene. recent demonstration DNA maternal plasma raises possibility that genotyping may be possible with use plasma.
Nanoparticle tracking analysis (NTA) is a light-scattering technique that useful for the rapid sizing and enumeration of extracellular vesicles (EVs). As relatively new method, NTA has been criticised lack standardisation. We propose use silica microspheres calibration measurements describe in detail protocol EVs by which should minimise many sources variability imprecision associated with this technique.
Abstract Background: There is much recent interest in the biologic and diagnostic implication of cell-free non-host DNA plasma serum human subjects. To determine if quantitative abnormalities circulating may be associated with certain pathologic processes, we used fetal preeclampsia as a model system. Methods: We studied 20 preeclamptic women control subjects comparable gestational age (means, 32 33 weeks, respectively). Male maternal was measured using real-time PCR for SRY gene on Y...
Background— Hypertensive disorders of pregnancy are a major contributor to death and disability for pregnant women their infants. The diagnosis preeclampsia by using blood pressure proteinuria is limited use because they tertiary, downstream features the disease. Placental growth factor (PlGF) an angiogenic factor, secondary marker associated placental dysfunction in preeclampsia, with known low plasma concentrations Methods Results— In prospective multicenter study, we studied diagnostic...
Abstract Systemic inflammatory responsiveness was studied in normal human pregnancy and its specific disorder, pre-eclampsia. Compared with nonpregnancy, monocytes were primed to produce more TNF-α throughout pregnancy, IL-12p70 the first second trimesters, IL-18 trimester only. Intracellular cytokine measurements (TNF-α IL12p70) showed little change by comparison. IFN-γ production suppressed all three trimesters. In pre-eclampsia, secretion increased. Secreted but not intracellular measures...
Generalised maternal endothelial cell dysfunction appears to be an underlying problem in pre-eclampsia presumed caused, directly or indirectly, by one more circulating factors derived from the placenta. Recently it has been suggested that tumour necrosis factor (TNF) may play important role and contribute activation. This study was designed investigate this proposal.Plasma TNF-alpha, IL-6 both forms of soluble TNF receptors (p55 p75 TNF-R) have measured ELISA 31 pre-eclamptic patients...