A5004950747- Aquaculture disease management and microbiota
- Gut microbiota and health
- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Probiotics and Fermented Foods
- Coral and Marine Ecosystems Studies
- Clostridium difficile and Clostridium perfringens research
- Diet and metabolism studies
- Marine Bivalve and Aquaculture Studies
- Vibrio bacteria research studies
- Microbial infections and disease research
- Myxozoan Parasites in Aquatic Species
- Gastrointestinal motility and disorders
- Marine Ecology and Invasive Species
- Photoreceptor and optogenetics research
- Photosynthetic Processes and Mechanisms
- Cancer Research and Treatments
- Identification and Quantification in Food
- Marine Sponges and Natural Products
- Metabolomics and Mass Spectrometry Studies
- Fisheries and Aquaculture Studies
- Marine and fisheries research
- Microbial Metabolites in Food Biotechnology
Temasek Life Sciences Laboratory
2017-2022
National University of Singapore
2017-2019
King Abdullah University of Science and Technology
2011-2017
Abstract Intestinal tracts are among the most densely populated microbial ecosystems. Gut microbiota and their influence on host have been well characterized in terrestrial vertebrates but much less so fish. This is especially true for coral reef fishes, which abundant groups of earth. Surgeonfishes (family: Acanthuridae) part a large diverse family fish that display wide range feeding behaviours, turn has strong impact ecology. Here, we studied composition gut nine surgeonfish three...
Despite advances in sequencing, lack of standardization makes comparisons across studies challenging and hampers insights into the structure function microbial communities multiple habitats on a planetary scale. Here we present multi-omics analysis diverse set 880 community samples collected for Earth Microbiome Project. We include amplicon (16S, 18S, ITS) shotgun metagenomic sequence data, untargeted metabolomics data (liquid chromatography-tandem mass spectrometry gas chromatography...
Significance Gastrointestinal symbionts of organisms are important in the breakdown food for host, particularly herbivores requiring exogenous enzymes to digest complex polysaccharides their diet. However, role digestion algae marine piscine remains unresolved. Here, we show that diversity sources available herbivorous surgeonfishes is directly linked with genetic makeup enteric microbiota. Importantly, genomic blueprint dominant belonging diverse Epulopiscium clades differs according host...
Epulopiscium is a group of giant bacteria found in high abundance intestinal tracts herbivorous surgeonfish. Despite their peculiarly large cell size (can be up to 600 µm), extreme polyploidy (some with over 100,000 genome copies per cell) and viviparity (whereby mother cells produce live offspring), details about diversity, distribution or role the host gut are lacking. Previous studies have highlighted existence morphologically distinct types (defined as morphotypes A J) surgeonfish...
The lifelong relationship between microorganisms and hosts has a profound impact on the overall health physiology of holobiont. Microbiome composition throughout life span host remains largely understudied. Here, fecal microbiota conventionally raised C57BL/6J male mice was characterized almost entire adult span, from "maturing" (9 weeks) until "very old" (112 age. Our results suggest that changes occur but are more pronounced in maturing to middle-age than later life. Phylum-level analysis...
Three bacterial strains, C9, H5 and TLL-A3, were isolated from fecal pellets of conventionally raised C57BL/6J mice. Analysis 16S rRNA genes indicated that the strains belonged to Muribaculaceae, shared 91.6-99.9 % sequence identity with recently described Duncaniella muris DSM 103720T. Genome-sequencing isolates was performed compare average nucleotide identities (ANI) between strains. The ANI analysis revealed all highest D. 103720T, strain C9 being most similar (ANI: 98.0 %) followed by...
An anaerobic bacterial strain, named TLL-A4T, was isolated from fecal pellets of conventionally raised C57BL/6J mice. Analysis the 16S rRNA gene indicated that strain belongs to phylum Bacteroidetes and, more specifically, recently proposed Muribaculaceae (also known as S24-7 clade or Candidatus Homeothermaceae). Strain TLL-A4T's shared 92.8 % sequence identity with type only published species genus Muribaculum, Muribaculum intestinale DSM 28989T. Genome-sequencing TLL-A4T performed compare...
Dietary changes are known to alter the composition of gut microbiome. However, it is less understood how repeatable and reversible these diet switches affect microbiota in various segments gastrointestinal tract. Here, a treatment group conventionally raised laboratory mice subjected two periods western (WD) interrupted by period standard (SD) same duration. Beta-diversity analyses show that diet-induced largely (
The use of gnotobiotics has attracted wide interest in recent years due to technological advances that have revealed the importance host-associated microbiomes for host physiology and health. One oldest most important gnotobiotic mouse model, altered Schaedler flora (ASF) been used several decades. ASF comprises eight different bacterial strains, which characterized extent, but only a few are available through public strain collections. Here, isolation close relative one less-studied...
Here, the complete genome sequence of Duncaniella muris strain B8 is presented. The anaerobic was isolated from feces C57/BL6 mice and closely related to D. DSM 103720, which type recently proposed genus Muribaculaceae .
ABSTRACT The life-long relationship between microorganisms and hosts has a profound impact on the overall health physiology of holobiont. Changes in microbiome composition throughout lifespan host remain, however, largely understudied. In this study, fecal conventionally raised C57BL/6J mice was analyzed almost entire expected lifespan, from ‘maturing’ (9 weeks) until ‘very old’ age (112 weeks). Analysis alpha beta diversity suggests that gradual changes occur murine life but appear to be...
Here, the complete genome sequence of sponge-associated Tenacibaculum mesophilum DSM 13764T is presented. T. a close relative fish pathogen maritimum, which causes significant disease outbreaks in aquaculture facilities. The will serve as an important resource for comparative genomics approaches.
Abstract Outbreaks of diseases in farmed fish remain a recurring problem despite the development vaccines and improved hygiene standards on aquaculture farms. One commonly observed bacterial disease tropical South-East Asian region is tenacibaculosis, which attributed to members Bacteroidetes genus Tenacibaculum , most notably T. maritimum . The impact tenacibaculosis microbiota remains poorly understood. In this study, we analysed different tissue types commercially reared seabass ( Lates...
Abstract Dietary changes are known to alter the composition of gut microbiome. However, it is less understood how repeatable and reversible these diet-switches affect microbiota in various segments gastrointestinal tract. Here, a treatment group conventionally raised laboratory mice subjected two periods western diet (WD) interrupted by period standard (SD) same duration. Beta-diversity analyses show that diet-induced largely ( q = 0.1501; PERMANOVA, weighted-UniFrac comparison treatment-SD...
The use of gnotobiotics has gained large interest in recent years due to technological advances that have revealed the importance host-associated microbiomes for host physiology and health. One oldest most important mouse model, Altered Schaedler Flora (ASF) been used several decades. ASF comprises eight different bacterial species, which characterized extent, but only few are available through public strain collections. Here, isolation a close relative one less studied strains, Clostridium...