A5067356378- Microbial Natural Products and Biosynthesis
- Marine Sponges and Natural Products
- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Magnetism in coordination complexes
- Synthetic Organic Chemistry Methods
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Metal-Organic Frameworks: Synthesis and Applications
- Metal complexes synthesis and properties
- Chemical Synthesis and Analysis
- Fungal Biology and Applications
- Lanthanide and Transition Metal Complexes
- Plant biochemistry and biosynthesis
- Crystallography and molecular interactions
- Carbohydrate Chemistry and Synthesis
- Porphyrin and Phthalocyanine Chemistry
- Synthesis and Biological Activity
- Supramolecular Chemistry and Complexes
- Bioactive Compounds and Antitumor Agents
- Organometallic Complex Synthesis and Catalysis
- Seaweed-derived Bioactive Compounds
- Metal-Catalyzed Oxygenation Mechanisms
- Molecular spectroscopy and chirality
- Microbial Metabolic Engineering and Bioproduction
University of California, San Diego
2016-2025
Scripps Institution of Oceanography
2016-2025
Sandia National Laboratories California
2021
The University of Sydney
2007-2015
University of California System
2006-2015
Monash University
2000-2011
University of Hawaii at Hilo
2008-2010
Korea Institute of Science and Technology
2010
Alfred-Wegener-Institut Helmholtz-Zentrum für Polar- und Meeresforschung
2010
UNSW Sydney
2008-2009
Strong and selective cytotoxicity toward a variety of cancer cell lines is shown by salinosporamide A (1), which was isolated from the culture extract unique marine actinomycete belonging to recently discovered genus Salinospora. We reported cultivation phylogenetic characterization new group obligate bacteria that widely distributed in ocean sediments.1 Analogous soil-derived actinomycetes have been single most significant source naturally occurring microbial antibiotics,2 thus discovery...
ABSTRACT A major taxon of obligate marine bacteria within the order Actinomycetales has been discovered from ocean sediments. Populations these (designated MAR 1) are persistent and widespread, spanning at least three distinct systems. In this study, 212 actinomycete isolates possessing 1 morphologies were examined all but two displayed an requirement seawater for growth. Forty-five isolates, representing observed seawater-requiring morphotypes, partially sequenced found to share...
Recent fermentation studies have identified actinomycetes of the marine-dwelling genus Salinispora as prolific natural product producers. To further evaluate their biosynthetic potential, we sequenced 5,183,331-bp S. tropica CNB-440 circular genome and analyzed all identifiable secondary gene clusters. Our analysis shows that dedicates a large percentage its ( approximately 9.9%) to assembly, which is greater than previous Streptomyces sequences well other product-producing actinomycetes....
New bioinformatic tools are needed to analyze the growing volume of DNA sequence data. This is especially true in case secondary metabolite biosynthesis, where highly repetitive nature associated genes creates major challenges for accurate assembly and analysis. Here we introduce web tool Natural Product Domain Seeker (NaPDoS), which provides an automated method assess biosynthetic gene diversity novelty strains or environments. NaPDoS analyses based on phylogenetic relationships tags...
[structure: see text] The synthesis and UV-vis NMR spectroscopic studies of thiourea-based colorimetric sensors for anions are presented. These can recognize through hydrogen binding even in competitive pH-buffered aqueous solutions, giving rise to large color changes that clearly visible the naked eye.
Major advances in our understanding of marine bacterial diversity have been gained through studies bacterioplankton, the vast majority which appear to be gram negative. Less effort has devoted bacteria inhabiting sediments, yet there is evidence suggest that gram-positive comprise a relatively large proportion these communities. To further expand aerobic present tropical culture-dependent approach was applied sediments collected Republic Palau from intertidal zone depths 500 m. This...
Cultivation of an obligate marine Streptomyces strain has furnished the marinopyrroles A and B, densely halogenated, axially chiral metabolites that contain uncommon bispyrrole structure. X-ray analysis marinopyrrole B showed natural product exists as atropo-enantiomer with M-configuration. Though configurationally stable at room temperature, M-(−)-marinopyrrole can be racemized elevated temperatures to yield non-natural P-(+)-atropo-enantiomer. The possess potent antibiotic activities...
Significance Microbial natural products are a major source of new drug leads, yet discovery efforts constrained by the lack information describing diversity and distributions associated biosynthetic pathways among bacteria. Using marine actinomycete genus Salinispora as model, we analyzed genome sequence data from 75 closely related strains. The results provide evidence for high levels pathway diversity, with most being acquired relatively recently in evolution genus. evolutionary histories...
Recent genome sequencing efforts have led to the rapid accumulation of uncharacterized or "orphaned" secondary metabolic biosynthesis gene clusters (BGCs) in public databases. This increase DNA-sequenced big data has given rise significant challenges applied field natural product mining, including (i) how prioritize characterization orphan BGCs and (ii) rapidly connect genes biosynthesized small molecules. Here, we show that by correlating putative antibiotic resistance encode...
Genomics and metabolomics are widely used to explore specialized metabolite diversity. The Paired Omics Data Platform is a community initiative systematically document links between metabolome (meta)genome data, aiding identification of natural product biosynthetic origins structures.
Despite advances in sequencing, lack of standardization makes comparisons across studies challenging and hampers insights into the structure function microbial communities multiple habitats on a planetary scale. Here we present multi-omics analysis diverse set 880 community samples collected for Earth Microbiome Project. We include amplicon (16S, 18S, ITS) shotgun metagenomic sequence data, untargeted metabolomics data (liquid chromatography-tandem mass spectrometry gas chromatography...
The isolation and structure determination of a new chlorinated benzophenone antibiotic, pestalone (1), is described. compound was produced by cultured marine fungus only when unicellular bacterium, strain CNJ-328, co-cultured in the fungal fermentation. fungus, isolated from surface brown alga Rosenvingea sp. collected Bahamas Islands, identified as an undescribed member genus Pestalotia. 1, initially assigned with modest confidence combined spectral chemical data, confirmed single-crystal...
A taxonomic study was carried out to clarify the taxonomy of representatives a group marine actinomycetes previously designated MAR 1 and considered belong family Micromonosporaceae . The organisms had phenotypic properties consistent with their assignment this taxon. strains formed distinct taxon in 16S rRNA gene tree shared range that distinguished them from members all genera validly published names classified family. name proposed for novel is Salinispora gen. nov. genus contains two...
Summary Actinomycetes were cultivated using a variety of media and selective isolation techniques from 275 marine samples collected around the island Guam. In total, 6425 actinomycete colonies observed 983 (15%) these, representing range morphological diversity each sample, obtained in pure culture. The majority strains isolated (58%) required seawater for growth indicating high degree adaptation. dominant recovered (568 strains) belonged to seawater‐requiring taxon ‘ Salinospora ’, new...
ABSTRACT Here we report associations between secondary metabolite production and phylogenetically distinct but closely related marine actinomycete species belonging to the genus Salinispora . The pattern emerged in a study that included global collection sites, it indicates can be species-specific, phenotypic trait associated with broadly distributed bacterial populations. Associations phylotype chemotype revealed an effective, diversity-based approach natural product discovery contradicts...
Actinomycetes were isolated from near-shore marine sediments collected at 15 island locations throughout the Bahamas. A total of 289 actinomycete colonies observed, and all but 6 could be assigned to suprageneric groups actinoplanetes streptomycetes. bimodal distribution in population relation depth was recorded, with maximum numbers occurring shallow deep sampling sites. This can accounted for by a rapid decrease streptomycetes an increase increasing does not conform theory that...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTEleutherobin, a New Cytotoxin that Mimics Paclitaxel (Taxol) by Stabilizing MicrotubulesThomas Lindel, Paul R. Jensen, William Fenical, Byron H. Long, Anna Maria Casazza, Joan Carboni, and Craig FairchildView Author Information Center for Marine Biotechnology Biomedicine Scripps Institution of Oceanography University CaliforniaSan Diego La Jolla, California 92093-0236 Oncology Drug Discovery, Bristol-Myers Squibb Pharmaceutical Research...
Induction of the production emericellamides A and B (1, 2), by marine-derived fungus Emericella sp., was observed during co-culture with marine actinomycete Salinispora arenicola. The planar structures these new cyclic depsipeptides, which incorporate 3-hydroxy-2,4-dimethyldecanoic acid 3-hydroxy-2,4,6-trimethyldodecanoic acid, were assigned combined chemical spectral methods. absolute configurations amino acids, those chiral centers on side chain, established application Marfey's method,...
Four antitumor-antibiotics of a new structure class, the marinomycins A-D (1-4), were isolated from saline culture group marine actinomycetes, for which we have proposed name "Marinispora". The structures marinomycins, are unusual macrodiolides composed dimeric 2-hydroxy-6-alkenyl-benzoic acid lactones with conjugated tetraene-pentahydroxy polyketide chains, assigned by combined spectral and chemical methods. In room light, marinomycin A slowly isomerizes to its geometrical isomers B C....